IPO5 importin 5 [Homo sapiens (human)] - Gene

Summary

Official Symbol: IPO5provided by HGNC
Official Full Name: importin 5provided by HGNC
Primary source: HGNC:HGNC:6402
See related: Ensembl:ENSG00000065150 MIM:602008; AllianceGenome:HGNC:6402
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: IMB3; Pse1; imp5; KPNB3; RANBP5
Summary: Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. [provided by RefSeq, Jul 2008]
Expression: Broad expression in testis (RPKM 84.9), ovary (RPKM 29.9) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 13q32.2

Exon count:: 32

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	13	NC_000013.11 (97953675..98024296)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	13	NC_060937.1 (97160508..97231138)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	13	NC_000013.10 (98605929..98676550)

Chromosome 13 - NC_000013.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

IPO5 promotes malignant progression of esophageal cancer through activating MMP7.
Title: IPO5 promotes malignant progression of esophageal cancer through activating MMP7.
X-ray Structure of the Human Karyopherin RanBP5, an Essential Factor for Influenza Polymerase Nuclear Trafficking.
Title: X-ray Structure of the Human Karyopherin RanBP5, an Essential Factor for Influenza Polymerase Nuclear Trafficking.
DDX56 inhibits type I interferon by disrupting assembly of IRF3-IPO5 to inhibit IRF3 nucleus import.
Title: DDX56 inhibits type I interferon by disrupting assembly of IRF3-IPO5 to inhibit IRF3 nucleus import.
IPO5 is an oncogene involved in CRC cell proliferation and migration. This highlights the significance of IPO5 in 5-fluorouracil-resistant CRC cells. The oncogenic function of IPO5 was mediated by promoting RAS signalling by increasing the nuclear translocation of RASAL2
Title: IPO5 promotes the proliferation and tumourigenicity of colorectal cancer cells by mediating RASAL2 nuclear transportation.
Results indicate that the interaction between FLIL33 and IPO5 is localized to a specific segment of the FLIL33 protein, is not required for nuclear localization of FLIL33, and protects FLIL33 from proteasome-dependent degradation.
Title: The full-length interleukin-33 (FLIL33)-importin-5 interaction does not regulate nuclear localization of FLIL33 but controls its intracellular degradation.
importin-alpha5, which is a key regulator of interferon signaling following Ebola virus infection, as one putative target of miRNA.
Title: Ebola virus encodes a miR-155 analog to regulate importin-α5 expression.
The nuclear importin IPO5 was identified as a novel interacting protein of SMAD1. Overexpression of IPO5 in various cell lines specifically increases nuclear localization of BMP receptor-activated SMADs (R-SMADs) confirming a functional relationship between IPO5 and BMP but not TGF-beta R-SMADs.
Title: Quantitative Proteomics of the SMAD (Suppressor of Mothers against Decapentaplegic) Transcription Factor Family Identifies Importin 5 as a Bone Morphogenic Protein Receptor SMAD-specific Importin.
Importins, Impbeta, Kapbeta2, Imp4, Imp5, Imp7, Imp9, and Impalpha, show the H3 tail binding more tightly than the H4 tail. The H3 tail binds Kapbeta2 and Imp5 with KD values of 77 and 57 nm, respectively, and binds the other five Importins more weakly.
Title: Recognition Elements in the Histone H3 and H4 Tails for Seven Different Importins.
Single nucleotide polymorphism in IPO5 gene is associated with Peripheral Arterial Disease.
Title: Genome-Wide Association Study of Peripheral Arterial Disease in a Japanese Population.
IQGAP1 interacts with human importin-beta5 in HEK 293T cells.
Title: IQGAP1 protein regulates nuclear localization of β-catenin via importin-β5 protein in Wnt signaling.

Submit: New GeneRIF Correction See all GeneRIFs (25)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Genome-wide association study of primary dentition pit-and-fissure and smooth surface caries. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Replication interactions

Interaction	Pubs
HIV-1 infection (VSV-G pseudotyped) of CEMT4 T cells downregulates plasma membrane expression of IPO5	PubMed

Protein interactions

Protein	Gene	Interaction	Pubs
Rev	rev	importin beta specifically binds to the Rev nuclear localization signal (NLS, amino acids 35-50) and mediates the nuclear import of Rev	PubMed
	rev	importin beta can inhibit the interaction of HIV-1 Rev with HIV-1 RRE RNA	PubMed
Tat	tat	The binding of HIV-1 Tat with importin beta is inhibited by RanGTP	PubMed
	tat	Importin beta binds to the HIV-1 Tat protein nuclear localization signal (NLS; amino acids 49-57) and mediates the nuclear import of Tat through a novel import pathway	PubMed
Vpr	vpr	HIV-1 Vpr causes increased levels of CyclinB1, Plk1, and Cdk1 in a complex with the nuclear transport and spindle assembly protein, importin beta	PubMed
	vpr	HIV-1 Vpr co-localizes with karyopherin beta and regulates karyopherin beta-mediated docking of the HIV-1 preintegration complex at the nuclear envelope, however multiple reports indicate the two proteins do not directly interact	PubMed
capsid	gag	The interaction of HIV-1 CA with human cellular importin 5 protein (IPO5) is identified by yeast two-hybrid screen	PubMed
integrase	gag-pol	Some reports indicate a possible role for the interactions between karyopherin alpha and beta with HIV-1 integrase in the nuclear import of HIV-1 preintegration complexes (PIC), while other reports indicate integrase is not involved in PIC nuclear import	PubMed
	gag-pol	Karyopherin beta binds to complexes between HIV-1 integrase (integrase/GST fusion protein) and karyopherin alpha, but not to integrase alone	PubMed
	gag-pol	Karyopherin alpha and beta are reported to interact with HIV-1 integrase (IN) to facilitate nuclear import of IN, however a conflicting report indicates nuclear accumulation of IN does not involve karyopherin alpha, beta 1, or beta 2 mediated pathways	PubMed
matrix	gag	Karyopherin beta mediates the nuclear import of HIV-1 preintegration complexes through karyopherin alpha, which binds directly to nuclear localization signals in HIV-1 Matrix	PubMed

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH44720 (e-PCR)
- UniSTS:48644

GDB:434012 (e-PCR)
- UniSTS:157204

STS-N89596 (e-PCR)
- UniSTS:17943

D8S2278 (e-PCR)
- UniSTS:473906

D8S2279 (e-PCR)
- UniSTS:473907

SHGC-85098 (e-PCR)
- UniSTS:103476

STS-D29365 (e-PCR)
- UniSTS:76362

L18426 (e-PCR)
- UniSTS:34648

RH15609 (e-PCR)
- UniSTS:84580

SHGC-80892 (e-PCR)
- UniSTS:102535

D11S3114 (e-PCR)
- UniSTS:152207

KPNB3_1159 (e-PCR)
- UniSTS:277442

D13S853E (e-PCR)
- UniSTS:151519

SGC33733 (e-PCR)
- UniSTS:42975

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables GTPase inhibitor activity	TAS Traceable Author Statement more info	PubMed
enables RNA binding	HDA more info	PubMed
enables nuclear import signal receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables nuclear import signal receptor activity	IDA Inferred from Direct Assay more info	PubMed
enables nuclear import signal receptor activity	ISS Inferred from Sequence or Structural Similarity more info
enables nuclear localization sequence binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables small GTPase binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in NLS-bearing protein import into nucleus	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular response to amino acid stimulus	ISS Inferred from Sequence or Structural Similarity more info
involved_in cytoplasmic pattern recognition receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of cyclin-dependent protein serine/threonine kinase activity	TAS Traceable Author Statement more info	PubMed
involved_in positive regulation of protein import into nucleus	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein import into nucleus	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein import into nucleus	IDA Inferred from Direct Assay more info	PubMed
involved_in ribosomal protein import into nucleus	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	IDA Inferred from Direct Assay more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in cytosol	IDA Inferred from Direct Assay more info
located_in membrane	HDA more info	PubMed
is_active_in nuclear membrane	IC Inferred by Curator more info	PubMed
located_in nuclear membrane	IDA Inferred from Direct Assay more info
part_of nuclear pore	TAS Traceable Author Statement more info	PubMed
located_in nucleolus	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	IDA Inferred from Direct Assay more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: importin-5

Names: Kap beta3 import receptor; PSE1 homolog; RAN binding protein 5; Ran_GTP binding protein 5; importin beta-3 subunit; importin subunit beta-3; karyopherin (importin) beta 3; karyopherin beta-3; ran-binding protein 5

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_002271.6 → NP_002262.4 importin-5

Status: REVIEWED

Source sequence(s)

AF339834, AK125031, AL137120, AL705630, BC000947, BC001497, BC045640, BU618294, Y08890

Consensus CDS

CCDS31999.2

UniProtKB/Swiss-Prot

B4DZA0, O00410, O15257, Q5T578, Q86XC7

UniProtKB/TrEMBL

H0Y8C6

Related

ENSP00000499125.1, ENST00000651721.2

Conserved Domains (6) summary

COG5215
Location:13 → 634

KAP95; Karyopherin (importin) beta [Intracellular trafficking and secretion]

sd00044
Location:355 → 387

HEAT; HEAT repeat [structural motif]

pfam13513
Location:921 → 966

HEAT_EZ; HEAT-like repeat

pfam18808
Location:268 → 357

Importin_rep_4; Importin repeat

pfam18816
Location:1005 → 1051

Importin_rep_5; Importin repeat

pfam18829
Location:765 → 874

Importin_rep_6; Importin repeat 6

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000013.11 Reference GRCh38.p14 Primary Assembly

Range

97953675..98024296

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_024449346.2 → XP_024305114.1 importin-5 isoform X1

UniProtKB/TrEMBL

H0Y8C6

Conserved Domains (6) summary

COG5215
Location:31 → 652

KAP95; Karyopherin (importin) beta [Intracellular trafficking and secretion]

sd00044
Location:373 → 405

HEAT; HEAT repeat [structural motif]

pfam13513
Location:939 → 984

HEAT_EZ; HEAT-like repeat

pfam18808
Location:286 → 375

Importin_rep_4; Importin repeat

pfam18816
Location:1023 → 1069

Importin_rep_5; Importin repeat

pfam18829
Location:783 → 892

Importin_rep_6; Importin repeat 6
XM_011521089.3 → XP_011519391.1 importin-5 isoform X1

See identical proteins and their annotated locations for XP_011519391.1

UniProtKB/TrEMBL

H0Y8C6

Conserved Domains (6) summary

COG5215
Location:31 → 652

KAP95; Karyopherin (importin) beta [Intracellular trafficking and secretion]

sd00044
Location:373 → 405

HEAT; HEAT repeat [structural motif]

pfam13513
Location:939 → 984

HEAT_EZ; HEAT-like repeat

pfam18808
Location:286 → 375

Importin_rep_4; Importin repeat

pfam18816
Location:1023 → 1069

Importin_rep_5; Importin repeat

pfam18829
Location:783 → 892

Importin_rep_6; Importin repeat 6
XM_047430298.1 → XP_047286254.1 importin-5 isoform X1
XM_047430299.1 → XP_047286255.1 importin-5 isoform X2

UniProtKB/Swiss-Prot

B4DZA0, O00410, O15257, Q5T578, Q86XC7

Related

ENSP00000350219.3, ENST00000357602.7
XM_011521087.3 → XP_011519389.1 importin-5 isoform X1

See identical proteins and their annotated locations for XP_011519389.1

UniProtKB/TrEMBL

H0Y8C6

Conserved Domains (6) summary

COG5215
Location:31 → 652

KAP95; Karyopherin (importin) beta [Intracellular trafficking and secretion]

sd00044
Location:373 → 405

HEAT; HEAT repeat [structural motif]

pfam13513
Location:939 → 984

HEAT_EZ; HEAT-like repeat

pfam18808
Location:286 → 375

Importin_rep_4; Importin repeat

pfam18816
Location:1023 → 1069

Importin_rep_5; Importin repeat

pfam18829
Location:783 → 892

Importin_rep_6; Importin repeat 6
XM_047430297.1 → XP_047286253.1 importin-5 isoform X1
XM_011521088.3 → XP_011519390.1 importin-5 isoform X1

See identical proteins and their annotated locations for XP_011519390.1

UniProtKB/TrEMBL

H0Y8C6

Conserved Domains (6) summary

COG5215
Location:31 → 652

KAP95; Karyopherin (importin) beta [Intracellular trafficking and secretion]

sd00044
Location:373 → 405

HEAT; HEAT repeat [structural motif]

pfam13513
Location:939 → 984

HEAT_EZ; HEAT-like repeat

pfam18808
Location:286 → 375

Importin_rep_4; Importin repeat

pfam18816
Location:1023 → 1069

Importin_rep_5; Importin repeat

pfam18829
Location:783 → 892

Importin_rep_6; Importin repeat 6
XM_047430296.1 → XP_047286252.1 importin-5 isoform X1
XM_017020562.2 → XP_016876051.1 importin-5 isoform X1

UniProtKB/TrEMBL

H0Y8C6

Related

ENSP00000261574.5, ENST00000261574.10

Conserved Domains (6) summary

COG5215
Location:31 → 652

KAP95; Karyopherin (importin) beta [Intracellular trafficking and secretion]

sd00044
Location:373 → 405

HEAT; HEAT repeat [structural motif]

pfam13513
Location:939 → 984

HEAT_EZ; HEAT-like repeat

pfam18808
Location:286 → 375

Importin_rep_4; Importin repeat

pfam18816
Location:1023 → 1069

Importin_rep_5; Importin repeat

pfam18829
Location:783 → 892

Importin_rep_6; Importin repeat 6
XM_005254052.4 → XP_005254109.1 importin-5 isoform X1

See identical proteins and their annotated locations for XP_005254109.1

UniProtKB/TrEMBL

H0Y8C6

Conserved Domains (6) summary

COG5215
Location:31 → 652

KAP95; Karyopherin (importin) beta [Intracellular trafficking and secretion]

sd00044
Location:373 → 405

HEAT; HEAT repeat [structural motif]

pfam13513
Location:939 → 984

HEAT_EZ; HEAT-like repeat

pfam18808
Location:286 → 375

Importin_rep_4; Importin repeat

pfam18816
Location:1023 → 1069

Importin_rep_5; Importin repeat

pfam18829
Location:783 → 892

Importin_rep_6; Importin repeat 6
XM_047430300.1 → XP_047286256.1 importin-5 isoform X2

UniProtKB/Swiss-Prot

B4DZA0, O00410, O15257, Q5T578, Q86XC7

Related

ENSP00000418393.1, ENST00000490680.5