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XPO1 exportin 1 [ Homo sapiens (human) ]

Gene ID: 7514, updated on 4-Oct-2020

Summary

Official Symbol
XPO1provided by HGNC
Official Full Name
exportin 1provided by HGNC
Primary source
HGNC:HGNC:12825
See related
Ensembl:ENSG00000082898 MIM:602559
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
emb; CRM1; exp1; CRM-1
Summary
This cell-cycle-regulated gene encodes a protein that mediates leucine-rich nuclear export signal (NES)-dependent protein transport. The protein specifically inhibits the nuclear export of Rev and U snRNAs. It is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. This protein also regulates NFAT and AP-1. [provided by RefSeq, Jan 2015]
Expression
Ubiquitous expression in testis (RPKM 38.6), lymph node (RPKM 33.8) and 25 other tissues See more
Orthologs

Genomic context

See XPO1 in Genome Data Viewer
Location:
2p15
Exon count:
31
Annotation release Status Assembly Chr Location
109.20200815 current GRCh38.p13 (GCF_000001405.39) 2 NC_000002.12 (61477849..61538522, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 2 NC_000002.11 (61705069..61765440, complement)

Chromosome 2 - NC_000002.12Genomic Context describing neighboring genes Neighboring gene ubiquitin specific peptidase 34 Neighboring gene small nucleolar RNA, H/ACA box 70B Neighboring gene USP34 divergent transcript Neighboring gene proteasome 26S subunit, non-ATPase 12 pseudogene Neighboring gene ribosomal protein S29 pseudogene 10

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIV-1 interactions

Replication interactions

Interaction Pubs
HIV-1 requires XPO1 for efficient replication as determined through the use of the XPO1 inhibitor KPT-185 PubMed
Knockdown of exportin 1 (CRM1 homolog; XPO1) by siRNA inhibits HIV-1 replication in HeLa P4/R5 cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Pr55(Gag) gag Human CRM1 exports gag mRNA to the cytoplasm more efficiently than rat CRM1 in rat cells, leading to efficient processing of Gag proteins and particle formation PubMed
gag Mouse-human CRM1 chimera analysis reveals that the amino-acid residues 146-444 of human CRM1 are important for increased HIV-1 titers in mouse cells. Three residues P411/M412/F414 are critical for human CRM1 function PubMed
gag Human CRM1 increases HIV-1 Gag production in mouse cells. Human CRM1 and SRp40 increase the amount of unspliced HIV-1 RNA and have a more-than-additive effect on HIV-1 titers in mouse cells PubMed
gag Human CRM1 enhances HIV-1 MA-dependent Gag membrane targeting in mouse cells PubMed
gag Expression of CRM1 enhances Gag production 10-15 fold in rat macrophages, but only marginally in rat T cells. Expression of both CRM1 and CycT1 factors synergistically enhances Gag production to levels approximately 10-40% of those detected in human cells PubMed
gag HIV-1 Matrix contains a nuclear export signal (amino acids 15-32) which is recognized by exportin 1 (CRM1; XPO1) and is important for nucleocytoplasmic shuttling of HIV-1 Gag and genomic RNA to the cytoplasm by exportin 1 PubMed
Rev rev A dimeric CRM1-RanGTP complex binds a Rev-RRE complex to export the Rev-RRE complex from the nuclear to the cytoplasm in cells PubMed
rev Computational molecular docking, alanine scanning, clustering, and evolutionary analysis reveal the interaction of DDX3 with HIV-1 Rev-CRM1-RanGTP complex PubMed
rev Co-immunoprecipitation analysis demonstrates that endogenous RBM14 associates with HIV-1 Rev, XPO1, and p54NRB in cells PubMed
rev The CRM1 dimer interface, including residues T346, P411, M412, F414, R474, E478, and H481, facilitates cooperative assembly with the Rev-RRE complex PubMed
rev CRM1-mediated nuclear export of Rev involves interactions of CRM1 with RanGTP, RanBP1 and RanGEF PubMed
rev CRM1 interacts with the nuclear export signal of Rev and acts as a bridge between Rev and nuclear pore proteins PubMed
rev Nuclear export of Rev is dependent on its activation domain which encodes a nuclear export signal (amino acids 75-83) PubMed
rev The addition of a second NES to HIV-1 Rev or a second RRE to Rev-dependent transcripts rescues HIV-1 virion production in murine cells independently of human CRM1 PubMed
rev HIV-1 Rev co-localizes with CRM1 at the HIV-1 transcription site in the nucleus PubMed
rev Human CRM1 rescues RRE/Rev-dependent HIV-1 virion production in mouse cells PubMed
rev Rev recruitment of CRM1 to HIV-1 mRNA is sufficient to mediate nuclear export of incompletely spliced HIV-1 mRNA PubMed
rev Ratjadone A (myxobacterial metabolite) interacts with CRM1 to inhibit the Rev-dependent HIV-1 RNA nuclear export PubMed
rev Eukaryotic initiation factor 5A (eIF-5A) mediates the binding of CRM1 to Rev PubMed
rev Interaction of CRM1 with nucleoporins Nup42 and Nup159 results in the release of Rev from CRM1 PubMed
rev During Rev-mediated nuclear export of HIV-1 mRNA, Rev interacts directly with the nucleocytoplasmic mRNA-transport system PubMed
rev Excess CRM1 may downregulate Rev-mediated RNA export by sequestering Rev to a subnucleolar structure PubMed
rev Nuclear retention and co-localization of Rev and CRM1 is caused by down-modulation of GAP-associated tyrosine phosphoprotein p62 (Sam68), suggesting Sam68 is directly involved in the CRM1-mediated Rev nuclear export pathway PubMed
rev HIV-1 Rev interacting protein, exportin 1 (XPO1), is identified by the in-vitro binding experiments involving cytosolic or nuclear extracts from HeLa cells PubMed
rev The HEAT repeat helix 9A domain (residues 402-423) of CRM1 serves as an effective cofactor for HIV-1 Rev function in higher primates PubMed
rev Three specific domains (residues 89-123, 405-593, and 594-670) of NF90 promote Rev-RRE mediated nuclear export of Gag mRNA, which is dependent on CRM1 function PubMed
rev HIV-1 Rev/CRM1 export complexes are observed in nucleoli by cotransfection with CFP/YFP pairs of the proteins on a large three-dimensional set of fixed cells, with Rev/CRM1 colocalization in nucleoli decreasing exponentially after addition of leptomycin B PubMed
rev Studies suggest Rev is necessary and sufficient for the accumulation of incompletely spliced HIV RNAs at nuclear pores while CRM1 is required for translocation across the nuclear pore complex PubMed
rev Rev-Rev interactions (multimerization) are enhanced by CRM1 PubMed
rev The C-terminal region of Rev (amino acids 86-116) plays a role in enhancing the interaction of Rev with CRM1 PubMed
rev CRM1 is involved in the binding of Rev to yeast Rip1 and hRip/Rab, which is involved in Rev-mediated nuclear export of HIV-1 mRNA PubMed
rev CRM1 interacts with FG-repeat-containing nuclear pore proteins during nuclear export of Rev PubMed
Tat tat Exportin 1 (XPO1, CRM1 homolog) is identified to interact with HIV-1 Tat mutant Nullbasic in HeLa cells by LC MS/MS PubMed
tat Interaction of HIV-1 Tat with XPO1 in T-cells is identified by a proteomic strategy based on affinity chromatography PubMed
Vpr vpr Nuclear export of HIV-1 Vpr by exportin-1 is required for efficient HIV replication in tissue macrophages and is important for Vpr incorporation into virions PubMed
vpr HIV-1 Vpr contains a nuclear export signal within amino acids 1-71 that is sensitive to Leptomycin B, suggesting it is recognized by CRM1 (exportin 1) PubMed
Vpu vpu HIV-1 Vpu is identified to have a physical interaction with exportin 1 (XPO1) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed
capsid gag Human CRM1 and SRp40 have a more-than-additive effect on HIV-1 CA production in mouse cells PubMed
matrix gag Human CRM1 enhances HIV-1 MA-dependent Gag membrane targeting in mouse cells PubMed
gag HIV-1 Matrix contains a nuclear export signal (amino acids 15-32) which is recognized by exportin 1 (CRM1; XPO1) and is important for nucleocytoplasmic shuttling of HIV-1 Gag and genomic RNA to the cytoplasm by exportin 1 PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • DKFZp686B1823

Gene Ontology Provided by GOA

Function Evidence Code Pubs
RNA binding IEA
Inferred from Electronic Annotation
more info
 
Ran GTPase binding IEA
Inferred from Electronic Annotation
more info
 
nuclear export signal receptor activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
nuclear export signal receptor activity IDA
Inferred from Direct Assay
more info
PubMed 
nuclear export signal receptor activity IMP
Inferred from Mutant Phenotype
more info
PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein domain specific binding IEA
Inferred from Electronic Annotation
more info
 
Process Evidence Code Pubs
intracellular transport of virus TAS
Traceable Author Statement
more info
 
mRNA transport IEA
Inferred from Electronic Annotation
more info
 
negative regulation of transcription by RNA polymerase II IEA
Inferred from Electronic Annotation
more info
 
nucleocytoplasmic transport IMP
Inferred from Mutant Phenotype
more info
PubMed 
protein export from nucleus IC
Inferred by Curator
more info
PubMed 
protein export from nucleus IDA
Inferred from Direct Assay
more info
PubMed 
protein export from nucleus IMP
Inferred from Mutant Phenotype
more info
PubMed 
protein export from nucleus IPI
Inferred from Physical Interaction
more info
PubMed 
protein localization to nucleus IEA
Inferred from Electronic Annotation
more info
 
regulation of centrosome duplication IEA
Inferred from Electronic Annotation
more info
 
regulation of mRNA stability TAS
Traceable Author Statement
more info
 
regulation of proteasomal ubiquitin-dependent protein catabolic process IDA
Inferred from Direct Assay
more info
PubMed 
regulation of protein export from nucleus IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
response to drug IEA
Inferred from Electronic Annotation
more info
 
ribosomal large subunit export from nucleus IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
ribosomal large subunit export from nucleus IMP
Inferred from Mutant Phenotype
more info
PubMed 
ribosomal small subunit export from nucleus IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
ribosomal small subunit export from nucleus IMP
Inferred from Mutant Phenotype
more info
PubMed 
ribosomal subunit export from nucleus IMP
Inferred from Mutant Phenotype
more info
PubMed 
ribosome biogenesis IMP
Inferred from Mutant Phenotype
more info
PubMed 
viral process TAS
Traceable Author Statement
more info
 
Component Evidence Code Pubs
Cajal body IEA
Inferred from Electronic Annotation
more info
 
annulate lamellae IDA
Inferred from Direct Assay
more info
PubMed 
cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
cytosol IDA
Inferred from Direct Assay
more info
 
cytosol TAS
Traceable Author Statement
more info
 
host cell IEA
Inferred from Electronic Annotation
more info
 
intracellular membrane-bounded organelle IDA
Inferred from Direct Assay
more info
 
kinetochore IDA
Inferred from Direct Assay
more info
PubMed 
membrane HDA PubMed 
nuclear envelope TAS
Traceable Author Statement
more info
PubMed 
nuclear membrane IDA
Inferred from Direct Assay
more info
 
nucleolus IDA
Inferred from Direct Assay
more info
PubMed 
nucleoplasm IDA
Inferred from Direct Assay
more info
 
nucleoplasm TAS
Traceable Author Statement
more info
 
nucleus IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
protein-containing complex IDA
Inferred from Direct Assay
more info
PubMed 
ribonucleoprotein complex IDA
Inferred from Direct Assay
more info
PubMed 

General protein information

Preferred Names
exportin-1
Names
chromosome region maintenance 1 homolog
chromosome region maintenance 1 protein homolog
exportin 1 (CRM1 homolog, yeast)
exportin-1 (required for chromosome region maintenance)

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_003400.4NP_003391.1  exportin-1

    See identical proteins and their annotated locations for NP_003391.1

    Status: REVIEWED

    Source sequence(s)
    AC016727, AK124769, AL833550, BC032847, BX492941, BX643790, BX647758, BX954288, CR749840, Y08614
    Consensus CDS
    CCDS33205.1
    UniProtKB/Swiss-Prot
    O14980
    UniProtKB/TrEMBL
    B3KWD0
    Related
    ENSP00000384863.2, ENST00000401558.7
    Conserved Domains (1) summary
    COG5101
    Location:151044
    CRM1; Importin beta-related nuclear transport receptor [Nuclear structure / Intracellular trafficking and secretion]

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.20200815

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000002.12 Reference GRCh38.p13 Primary Assembly

    Range
    61477849..61538522 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011533097.1XP_011531399.1  exportin-1 isoform X1

    See identical proteins and their annotated locations for XP_011531399.1

    UniProtKB/Swiss-Prot
    O14980
    Related
    ENSP00000385559.1, ENST00000406957.5
    Conserved Domains (1) summary
    COG5101
    Location:151044
    CRM1; Importin beta-related nuclear transport receptor [Nuclear structure / Intracellular trafficking and secretion]
  2. XM_005264544.2XP_005264601.1  exportin-1 isoform X2

    Conserved Domains (4) summary
    smart00913
    Location:46112
    IBN_N; Importin-beta N-terminal domain
    COG5101
    Location:151029
    CRM1; Importin beta-related nuclear transport receptor [Nuclear structure / Intracellular trafficking and secretion]
    pfam08389
    Location:110253
    Xpo1; Exportin 1-like protein
    pfam08767
    Location:6941011
    CRM1_C; CRM1 C terminal
  3. XM_011533098.2XP_011531400.1  exportin-1 isoform X3

    See identical proteins and their annotated locations for XP_011531400.1

    Conserved Domains (1) summary
    COG5101
    Location:15999
    CRM1; Importin beta-related nuclear transport receptor [Nuclear structure / Intracellular trafficking and secretion]
  4. XM_011533099.3XP_011531401.1  exportin-1 isoform X4

    See identical proteins and their annotated locations for XP_011531401.1

    Conserved Domains (4) summary
    smart00913
    Location:1046
    IBN_N; Importin-beta N-terminal domain
    COG5101
    Location:10978
    CRM1; Importin beta-related nuclear transport receptor [Nuclear structure / Intracellular trafficking and secretion]
    pfam08389
    Location:57202
    Xpo1; Exportin 1-like protein
    pfam08767
    Location:643960
    CRM1_C; CRM1 C terminal
  5. XM_006712094.3XP_006712157.1  exportin-1 isoform X1

    See identical proteins and their annotated locations for XP_006712157.1

    UniProtKB/Swiss-Prot
    O14980
    Conserved Domains (1) summary
    COG5101
    Location:151044
    CRM1; Importin beta-related nuclear transport receptor [Nuclear structure / Intracellular trafficking and secretion]
  6. XM_024453125.1XP_024308893.1  exportin-1 isoform X1

    Related
    ENSP00000385942.2, ENST00000404992.6
    Conserved Domains (1) summary
    COG5101
    Location:151044
    CRM1; Importin beta-related nuclear transport receptor [Nuclear structure / Intracellular trafficking and secretion]
  7. XM_024453126.1XP_024308894.1  exportin-1 isoform X1

    Conserved Domains (1) summary
    COG5101
    Location:151044
    CRM1; Importin beta-related nuclear transport receptor [Nuclear structure / Intracellular trafficking and secretion]
  8. XM_024453127.1XP_024308895.1  exportin-1 isoform X5

    Conserved Domains (1) summary
    COG5101
    Location:1913
    CRM1; Importin beta-related nuclear transport receptor [Nuclear structure / Intracellular trafficking and secretion]
  9. XM_005264546.2XP_005264603.1  exportin-1 isoform X5

    See identical proteins and their annotated locations for XP_005264603.1

    Conserved Domains (1) summary
    COG5101
    Location:1913
    CRM1; Importin beta-related nuclear transport receptor [Nuclear structure / Intracellular trafficking and secretion]
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