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Series GSE75765 Query DataSets for GSE75765
Status Public on Dec 31, 2015
Title Biogenesis and function of tRNA fragments during sperm maturation and fertilization in mammals (single embryo)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Parental dietary conditions can influence the metabolic traits of offspring. In mice, paternal consumption of low protein diet alters cholesterol and lipid metabolism of progeny. Here, we examine RNA species expressed in male reproductive tissues of mice. Protein restriction leads to altered levels of multiple small RNAs in mature sperm, as well as throughout the male reproductive tract, with decreased levels of let-7 family members and increased levels of 5’ fragments of tRNA-Gly isoacceptors. Intriguingly, tRNA fragments are scarce in the testis, but their levels increase in sperm during post-testicular maturation in the epididymis. We find that epididymosomes – extracellular vesicles which fuse with sperm during epididymal transit – exhibit RNA payloads closely matching those of mature sperm, and can deliver tRNA fragments to immature sperm in vitro both in mouse and in bull. Finally, we show that tRNA-Gly-GCC fragments play a role in repressing genes associated with the endogenous retroelement MERVL, both in ES cells and in preimplantation embryos. Our results shed light on small RNA biogenesis during post-testicular sperm maturation, and link tRNA fragments to regulation of endogenous retroelements active in the early embryo.
Overall design Zygotes were generated by IVF from animals fed a control diet. These embryos were then microinjected with various combinations of small RNAs and control RNA (HIS3.3::GFP). Follwoing injections the zygotes were developed and allowed to develop until 2 cell (2C) or 4 cell (4C) stage when single embryo RNA seq was carried out to study gene expression changes
Contributor(s) Sharma U, Conine C, Boskovic A, Rando O
Citation(s) 26721685
Submission date Dec 07, 2015
Last update date May 15, 2019
Contact name Upasna Sharma
Phone 508 856 6337
Organization name Umass Med School
Department BMP
Street address 364 Plantation Street
City Worcester
State/province MA
ZIP/Postal code 01605
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (187)
GSM1966922 C1_4C_10_0126
GSM1966923 C1_4C_37_0311
GSM1966924 C1_4C_39_0325
This SubSeries is part of SuperSeries:
GSE75622 Biogenesis and function of tRNA fragments during sperm maturation and fertilization in mammals
BioProject PRJNA305299
SRA SRP067082

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Supplementary file Size Download File type/resource
GSE75765_Sharma_Rando_2015_AStRF.xlsx 12.2 Mb (ftp)(http) XLSX
GSE75765_Sharma_Rando_2015_spermRNAinject.xlsx 4.7 Mb (ftp)(http) XLSX
GSE75765_Sharma_Rando_2015_tRFGGinject.xlsx 2.4 Mb (ftp)(http) XLSX
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Processed data are available on Series record

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