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    MTF-1 Metal response element-binding Transcription Factor-1 [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 39089, updated on 10-May-2024

    Summary

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    Official Symbol
    MTF-1provided by FlyBase
    Official Full Name
    Metal response element-binding Transcription Factor-1provided by FlyBase
    Primary source
    FLYBASE:FBgn0040305
    Locus tag
    Dmel_CG3743
    See related
    AllianceGenome:FB:FBgn0040305
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    anon-WO0118547.411; CG3743; Dmel\CG3743; dMTF-1; mtf-1; Mtf-1
    Summary
    Enables DNA-binding transcription factor activity and transcription cis-regulatory region binding activity. Involved in several processes, including metal ion homeostasis; response to copper ion; and stress response to cadmium ion. Located in cytoplasm and nucleus. Is expressed in several structures, including embryonic/larval garland cell; embryonic/larval midgut; embryonic/larval muscle system; garland cell primordium; and presumptive embryonic/larval digestive system. Orthologous to human MTF1 (metal regulatory transcription factor 1). [provided by Alliance of Genome Resources, Apr 2022]
    Orthologs
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    Genomic context

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    See MTF-1 in Genome Data Viewer
    Location:
    67B5-67B6; 3-29 cM
    Exon count:
    7
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 3L NT_037436.4 (9430312..9439490)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) 3L NT_037436.3 (9423412..9432590)

    Chromosome 3L - NT_037436.4Genomic Context describing neighboring genes Neighboring gene SHC-adaptor protein Neighboring gene Ribosomal protein S17 Neighboring gene uncharacterized protein Neighboring gene antisense RNA:CR45872 Neighboring gene Blocked early in transport 3 Neighboring gene Nuclear factor Y-box A Neighboring gene Sting-regulated gene 3

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. An in-vivo microfluidic assay reveals cardiac toxicity of heavy metals and the protective effect of metal responsive transcription factor (MTF-1) in Drosophila model. Zabihihesari A, et al. 3 Biotech, 2022 Oct. PMID 36275358, Free PMC Article
    2. Gawky modulates MTF-1-mediated transcription activation and metal discrimination. Jia R, et al. Nucleic Acids Res, 2021 Jun 21. PMID 34107019, Free PMC Article
    3. Mercury and cadmium trigger expression of the copper importer Ctr1B, which enables Drosophila to thrive on heavy metal-loaded food. Balamurugan K, et al. Biol Chem, 2009 Feb. PMID 19040355
    4. Overexpression of metal-responsive transcription factor (MTF-1) in Drosophila melanogaster ameliorates life-span reductions associated with oxidative stress and metal toxicity. Bahadorani S, et al. Neurobiol Aging, 2010 Jul. PMID 18775584
    5. Expression and localisation of the essential copper transporter DmATP7 in Drosophila neuronal and intestinal tissues. Burke R, et al. Int J Biochem Cell Biol, 2008. PMID 18321764

    See all (80) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Gawky modulates MTF-1-mediated transcription activation and metal discrimination.
      Title: Gawky modulates MTF-1-mediated transcription activation and metal discrimination.
    2. according to these results, a reduced expression of at least some of the zinc transporters is sufficient to normalize iron content. We also found that overexpression of MTF-1 improves the iron accumulation phenotype as well. In contrast, knocking down MtnA had no effect on the iron content
      Title: Metal Homeostasis Regulators Suppress FRDA Phenotypes in a Drosophila Model of the Disease.
    3. In addition, both metal specific domains are autorepressive in the absence of metal and contribute to the low level of basal transcription from metal inducible promoters.
      Title: Efficient metal-specific transcription activation by Drosophila MTF-1 requires conserved cysteine residues in the carboxy-terminal domain.
    4. Data indicate that MTF-1 is capable of binding to different regions of MtnA and Ctr1b genes depending on different metal stimulation.
      Title: Single nucleotide in the MTF-1 binding site can determine metal-specific transcription activation.
    5. Activation of this gene induced under opposite conditions of copper load and copper starvation manifested itself in a shortened lifespan, crippled wings, and female sterility.
      Title: Dissection of Drosophila MTF-1 reveals a domain for differential target gene activation upon copper overload vs. copper starvation.
    6. propose that Parkin and MTF-1 provide complementary functions in metal homeostasis, oxidative stress and other cellular stress response
      Title: The parkin mutant phenotype in the fly is largely rescued by metal-responsive transcription factor (MTF-1).
    7. When metal homeostasis is compromised by a mutation in metal-responsive transcription factor MTF-1, the life-span of the fly is shortened.
      Title: Overexpression of metal-responsive transcription factor (MTF-1) in Drosophila melanogaster ameliorates life-span reductions associated with oxidative stress and metal toxicity.
    8. Characterization of a novel cysteine-rich domain which is crucial for sensing excess intracellular copper by dMTF-1.
      Title: Copper sensing function of Drosophila metal-responsive transcription factor-1 is mediated by a tetranuclear Cu(I) cluster.
    9. The MTF-1's major role in both organisms is to control the transcription of genes involved in the homeostasis and detoxification of heavy metal ions such as Cu2+, Zn2+ and Cd2+.
      Title: Metal-responsive transcription factor (MTF-1) and heavy metal stress response in Drosophila and mammalian cells: a functional comparison.
    10. knockout of the protein in Drosophila by homologous recombination reveals its central role in heavy metal homeostasis
      Title: Knockout of 'metal-responsive transcription factor' MTF-1 in Drosophila by homologous recombination reveals its central role in heavy metal homeostasis.
    Submit: New GeneRIF Correction See all GeneRIFs (11)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    enables DNA-binding transcription factor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables transcription cis-regulatory region binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in cellular response to oxidative stress IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of transcription by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of transcription by RNA polymerase II IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in response to copper ion IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in response to metal ion IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in stress response to metal ion IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm HDA PubMed 
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    metal response element-binding transcription Factor-1
    Names
    CG3743-PA
    CG3743-PB
    CG3743-PC
    CG3743-PD
    CG3743-PE
    CG3743-PF
    CG3743-PG
    MTF-1-PA
    MTF-1-PB
    MTF-1-PC
    MTF-1-PD
    MTF-1-PE
    MTF-1-PF
    MTF-1-PG
    Metal-responsive transcription factor 1
    metal response element binding transcription factor
    metal response element binding transcription factor 1
    metal response element-binding transcription factor-1
    metal responsive transcription factor
    metal responsive transcription factor 1
    metal-regulatory transcription factor 1
    metal-responsive transcription factor-1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NT_037436.4 Reference assembly

      Range
      9430312..9439490
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001300073.1 → NP_001287002.1  metal response element-binding transcription Factor-1, isoform F [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001287002.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      X2JCQ6
      Conserved Domains (5) summary
      COG5048
      Location:442 → 519
      COG5048; FOG: Zn-finger [General function prediction only]
      sd00017
      Location:420 → 440
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam00096
      Location:418 → 440
      zf-C2H2; Zinc finger, C2H2 type
      pfam13465
      Location:432 → 458
      zf-H2C2_2; Zinc-finger double domain
      pfam17017
      Location:329 → 471
      zf-C2H2_aberr; Aberrant zinc-finger

    2. NM_001104090.2 → NP_001097560.1  metal response element-binding transcription Factor-1, isoform C [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001097560.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A8JNP3
      Conserved Domains (6) summary
      COG5048
      Location:442 → 519
      COG5048; FOG: Zn-finger [General function prediction only]
      sd00017
      Location:420 → 440
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam00096
      Location:418 → 440
      zf-C2H2; Zinc finger, C2H2 type
      pfam12446
      Location:862 → 931
      DUF3682; Protein of unknown function (DUF3682)
      pfam13465
      Location:432 → 458
      zf-H2C2_2; Zinc-finger double domain
      pfam17017
      Location:329 → 471
      zf-C2H2_aberr; Aberrant zinc-finger

    3. NM_001104091.2 → NP_001097561.1  metal response element-binding transcription Factor-1, isoform D [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001097561.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q8IQC5, Q95TF7
      Conserved Domains (4) summary
      COG5048
      Location:6 → 83
      COG5048; FOG: Zn-finger [General function prediction only]
      sd00017
      Location:12 → 34
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam12446
      Location:426 → 495
      DUF3682; Protein of unknown function (DUF3682)
      pfam13465
      Location:26 → 53
      zf-H2C2_2; Zinc-finger double domain

    4. NM_001300074.1 → NP_001287003.1  metal response element-binding transcription Factor-1, isoform G [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001287003.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q8IQC5, Q95TF7
      Conserved Domains (4) summary
      COG5048
      Location:6 → 83
      COG5048; FOG: Zn-finger [General function prediction only]
      sd00017
      Location:12 → 34
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam12446
      Location:426 → 495
      DUF3682; Protein of unknown function (DUF3682)
      pfam13465
      Location:26 → 53
      zf-H2C2_2; Zinc-finger double domain

    5. NM_140054.3 → NP_648311.2  metal response element-binding transcription Factor-1, isoform A [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_648311.2

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q9GRX2, Q9VSY7
      Related
      FBpp0076187
      Conserved Domains (6) summary
      COG5048
      Location:227 → 304
      COG5048; FOG: Zn-finger [General function prediction only]
      sd00017
      Location:205 → 225
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam00096
      Location:203 → 225
      zf-C2H2; Zinc finger, C2H2 type
      pfam12446
      Location:647 → 716
      DUF3682; Protein of unknown function (DUF3682)
      pfam13465
      Location:217 → 243
      zf-H2C2_2; Zinc-finger double domain
      pfam17017
      Location:114 → 256
      zf-C2H2_aberr; Aberrant zinc-finger

    6. NM_168343.2 → NP_729491.1  metal response element-binding transcription Factor-1, isoform B [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_729491.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q8IQC5, Q95TF7
      Related
      FBpp0076188
      Conserved Domains (4) summary
      COG5048
      Location:6 → 83
      COG5048; FOG: Zn-finger [General function prediction only]
      sd00017
      Location:12 → 34
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam12446
      Location:426 → 495
      DUF3682; Protein of unknown function (DUF3682)
      pfam13465
      Location:26 → 53
      zf-H2C2_2; Zinc-finger double domain

    7. NM_001104092.2 → NP_001097562.1  metal response element-binding transcription Factor-1, isoform E [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001097562.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q8IQC5, Q95TF7
      Conserved Domains (4) summary
      COG5048
      Location:6 → 83
      COG5048; FOG: Zn-finger [General function prediction only]
      sd00017
      Location:12 → 34
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam12446
      Location:426 → 495
      DUF3682; Protein of unknown function (DUF3682)
      pfam13465
      Location:26 → 53
      zf-H2C2_2; Zinc-finger double domain
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    A8JNP3 A8JNP3
    Q8IQC5 Q8IQC5
    Q95TF7 Q95TF7
    Q9GRX2 Q9GRX2
    Q9NFS1 Q9NFS1
    Q9VSY7 Q9VSY7
    X2JCQ6 X2JCQ6

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