NPAS2 neuronal PAS domain protein 2 [Homo sapiens (human)] - Gene

Summary

Official Symbol: NPAS2provided by HGNC
Official Full Name: neuronal PAS domain protein 2provided by HGNC
Primary source: HGNC:HGNC:7895
See related: Ensembl:ENSG00000170485 MIM:603347
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: MOP4; PASD4; bHLHe9
Summary: The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH)-PAS family of transcription factors. A similar mouse protein may play a regulatory role in the acquisition of specific types of memory. It also may function as a part of a molecular clock operative in the mammalian forebrain. [provided by RefSeq, Jul 2008]
Expression: Ubiquitous expression in esophagus (RPKM 4.7), urinary bladder (RPKM 4.5) and 22 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 2q11.2

Exon count:: 25

Annotation release	Status	Assembly	Chr	Location
109.20210514	current	GRCh38.p13 (GCF_000001405.39)	2	NC_000002.12 (100820139..100996829)
105.20201022	previous assembly	GRCh37.p13 (GCF_000001405.25)	2	NC_000002.11 (101436601..101613291)

Chromosome 2 - NC_000002.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Circadian genes polymorphisms, night work and prostate cancer risk: Findings from the EPICAP study.
Title: Circadian genes polymorphisms, night work and prostate cancer risk: Findings from the EPICAP study.
Our findings demonstrate that NPAS2 plays a critical role in glucose metabolism reprogramming, suggesting that NPAS2 may serve as a potential therapeutic target in HCC.
Title: Circadian clock gene NPAS2 promotes reprogramming of glucose metabolism in hepatocellular carcinoma cells.
Study reported an association between the risk of prostate cancer and the circadian pathway where significant associations were found for the genes NPAS2 and PER1.
Title: Circadian genes and risk of prostate cancer: Findings from the EPICAP study.
NPAS2 hypomethylation occurs at the early stage of PD and is a moderate biomarker for distinguishing PD patients from healthy subjects.
Title: Pyrosequencing analysis of methylation levels of clock genes in leukocytes from Parkinson's disease patients.
NPAS2 has a critical role in HCC cell survival and tumor growth, which is mainly mediated by transcriptional upregulation of CDC25A.
Title: NPAS2 promotes cell survival of hepatocellular carcinoma by transactivating CDC25A.
Aggregate genetic variation in circadian rhythm and melatonin pathways were significantly associated with the risk of prostate cancer in data combining GAME-ON and PLCO, after Bonferroni correction (ppathway < 0.00625). The two most significant genes were NPAS2 (pgene = 0.0062) and AANAT (pgene = 0.00078); the latter being significant after Bonferroni correction.
Title: Inherited variation in circadian rhythm genes and risks of prostate cancer and three other cancer sites in combined cancer consortia.
Significantly, this study is the first to show that a variant copy number GGC repeat sequence in the NPAS2 clock gene associates with melanoma risk and which may be useful in the assessment of melanoma predisposition.
Title: A polymorphic GGC repeat in the NPAS2 gene and its association with melanoma.
CLOCK, ARNTL, and NPAS2 gene polymorphisms may have a role in seasonal variations in mood and behavior
Title: Association of CLOCK, ARNTL, and NPAS2 gene polymorphisms and seasonal variations in mood and behavior.
Genetic variations in NPAS2 might be a biomarker for a seasonal pattern in bipolar disorders.
Title: Genetic association study of circadian genes with seasonal pattern in bipolar disorders.
With whole-exome sequencing a novel mutation in NPAS2 was identified in a Turkish family with nonobstructive azoospermia.
Title: Whole-exome sequencing identifies novel homozygous mutation in NPAS2 in family with nonobstructive azoospermia.

Submit: New GeneRIF Correction See all GeneRIFs (34)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Associated conditions

Description	Tests
Common genetic variants associated with cognitive performance identified using the proxy-phenotype method. GeneReviews: Not available
Genome-wide study links MTMR7 gene to variant Creutzfeldt-Jakob risk. GeneReviews: Not available

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH79879 (e-PCR)
- UniSTS:85760

SHGC-33168 (e-PCR)
- UniSTS:6424

D10S275 (e-PCR)
- UniSTS:147992

SHGC-85091 (e-PCR)
- UniSTS:103471

D8S2279 (e-PCR)
- UniSTS:473907

WI-17613 (e-PCR)
- UniSTS:32310

SHGC-78601 (e-PCR)
- UniSTS:101706

SHGC-34190 (e-PCR)
- UniSTS:5223

RH71260 (e-PCR)
- UniSTS:42346

SHGC-58283 (e-PCR)
- UniSTS:34702

RH17865 (e-PCR)
- UniSTS:68248

RH16198 (e-PCR)
- UniSTS:71326

PMC19579P4 (e-PCR)
- UniSTS:271786

RH77872 (e-PCR)
- UniSTS:69042

RH45156 (e-PCR)
- UniSTS:29379

SHGC-36364 (e-PCR)
- UniSTS:20938

SHGC-132981 (e-PCR)
- UniSTS:170963

SGC34754 (e-PCR)
- UniSTS:5596

Homology

Homologs of the NPAS2 gene: The NPAS2 gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, and frog.
Orthologs from Annotation Pipeline: 268 organisms have orthologs with human gene NPAS2
Orthologs

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IPI Inferred from Physical Interaction more info	PubMed
enables DNA-binding transcription factor activity	TAS Traceable Author Statement more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	ISA Inferred from Sequence Alignment more info
enables Hsp90 protein binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	ISS Inferred from Sequence or Structural Similarity more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein dimerization activity	IEA Inferred from Electronic Annotation more info
enables sequence-specific double-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to DNA damage stimulus	IEA Inferred from Electronic Annotation more info
involved_in central nervous system development	TAS Traceable Author Statement more info	PubMed
involved_in circadian regulation of gene expression	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in circadian regulation of gene expression	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of cell death	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of behavioral fear response	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription, DNA-templated	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription, DNA-templated	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of response to DNA damage stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in response to drug	ISS Inferred from Sequence or Structural Similarity more info
involved_in response to redox state	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of CLOCK-BMAL transcription complex	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in chromatin	ISA Inferred from Sequence Alignment more info
located_in cytosol	IDA Inferred from Direct Assay more info
located_in nucleoplasm	IDA Inferred from Direct Assay more info
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
part_of transcription regulator complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: neuronal PAS domain-containing protein 2

Names: PAS domain-containing protein 4; basic-helix-loop-helix-PAS protein MOP4; class E basic helix-loop-helix protein 9; member of PAS protein 4; member of PAS superfamily 4; neuronal PAS2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_023259.1 RefSeqGene

Range

4989..181679

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_002518.4 → NP_002509.2 neuronal PAS domain-containing protein 2

See identical proteins and their annotated locations for NP_002509.2

Status: REVIEWED

Source sequence(s)

AC016738, AC092168, AK095806, AK128586, BC072383, BM724652, U77970

Consensus CDS

CCDS2048.1

UniProtKB/Swiss-Prot

Q99743

UniProtKB/TrEMBL

A2I2P5

Related

ENSP00000338283.5, ENST00000335681.10

Conserved Domains (5) summary

smart00091
Location:84 → 149

PAS; PAS domain

cd00083
Location:7 → 61

HLH; Helix-loop-helix domain, found in specific DNA- binding proteins that act as transcription factors; 60-100 amino acids long. A DNA-binding basic region is followed by two alpha-helices separated by a variable loop region; HLH forms homo- and heterodimers, ...

cd00130
Location:250 → 350

PAS; PAS domain; PAS motifs appear in archaea, eubacteria and eukarya. Probably the most surprising identification of a PAS domain was that in EAG-like K+-channels. PAS domains have been found to bind ligands, and to act as sensors for light and oxygen in ...

pfam13426
Location:94 → 195

PAS_9; PAS domain

pfam14598
Location:249 → 352

PAS_11; PAS domain

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.20210514

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

NC_000002.12 Reference GRCh38.p13 Primary Assembly

Range

100820139..100996829

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011511242.2 → XP_011509544.1 neuronal PAS domain-containing protein 2 isoform X5

Conserved Domains (3) summary

cd00083
Location:7 → 61

HLH; Helix-loop-helix domain, found in specific DNA- binding proteins that act as transcription factors; 60-100 amino acids long. A DNA-binding basic region is followed by two alpha-helices separated by a variable loop region; HLH forms homo- and heterodimers, ...

cd00130
Location:220 → 320

PAS; PAS domain; PAS motifs appear in archaea, eubacteria and eukarya. Probably the most surprising identification of a PAS domain was that in EAG-like K+-channels. PAS domains have been found to bind ligands, and to act as sensors for light and oxygen in ...

pfam14598
Location:219 → 322

PAS_11; PAS domain
XM_005263953.2 → XP_005264010.1 neuronal PAS domain-containing protein 2 isoform X1

Conserved Domains (5) summary

smart00091
Location:149 → 214

PAS; PAS domain

cd00083
Location:72 → 126

HLH; Helix-loop-helix domain, found in specific DNA- binding proteins that act as transcription factors; 60-100 amino acids long. A DNA-binding basic region is followed by two alpha-helices separated by a variable loop region; HLH forms homo- and heterodimers, ...

cd00130
Location:315 → 415

PAS; PAS domain; PAS motifs appear in archaea, eubacteria and eukarya. Probably the most surprising identification of a PAS domain was that in EAG-like K+-channels. PAS domains have been found to bind ligands, and to act as sensors for light and oxygen in ...

pfam13426
Location:159 → 260

PAS_9; PAS domain

pfam14598
Location:314 → 417

PAS_11; PAS domain
XM_017004214.1 → XP_016859703.1 neuronal PAS domain-containing protein 2 isoform X2
XM_005263960.2 → XP_005264017.1 neuronal PAS domain-containing protein 2 isoform X7

Conserved Domains (3) summary

cd00083
Location:72 → 126

HLH; Helix-loop-helix domain, found in specific DNA- binding proteins that act as transcription factors; 60-100 amino acids long. A DNA-binding basic region is followed by two alpha-helices separated by a variable loop region; HLH forms homo- and heterodimers, ...

cd00130
Location:158 → 312

PAS; PAS domain; PAS motifs appear in archaea, eubacteria and eukarya. Probably the most surprising identification of a PAS domain was that in EAG-like K+-channels. PAS domains have been found to bind ligands, and to act as sensors for light and oxygen in ...

pfam13426
Location:159 → 260

PAS_9; PAS domain
XM_005263959.2 → XP_005264016.1 neuronal PAS domain-containing protein 2 isoform X4

Conserved Domains (6) summary

smart00091
Location:149 → 214

PAS; PAS domain

cd00083
Location:72 → 126

HLH; Helix-loop-helix domain, found in specific DNA- binding proteins that act as transcription factors; 60-100 amino acids long. A DNA-binding basic region is followed by two alpha-helices separated by a variable loop region; HLH forms homo- and heterodimers, ...

cd00130
Location:315 → 415

PAS; PAS domain; PAS motifs appear in archaea, eubacteria and eukarya. Probably the most surprising identification of a PAS domain was that in EAG-like K+-channels. PAS domains have been found to bind ligands, and to act as sensors for light and oxygen in ...

pfam06752
Location:590 → 719

E_Pc_C; Enhancer of Polycomb C-terminus

pfam13426
Location:159 → 260

PAS_9; PAS domain

pfam14598
Location:314 → 417

PAS_11; PAS domain
XM_017004216.1 → XP_016859705.1 neuronal PAS domain-containing protein 2 isoform X6
XM_017004217.1 → XP_016859706.1 neuronal PAS domain-containing protein 2 isoform X8
XM_017004215.1 → XP_016859704.1 neuronal PAS domain-containing protein 2 isoform X3
XM_011511243.2 → XP_011509545.1 neuronal PAS domain-containing protein 2 isoform X10

Conserved Domains (5) summary

smart00091
Location:149 → 214

PAS; PAS domain

cd00083
Location:72 → 126

HLH; Helix-loop-helix domain, found in specific DNA- binding proteins that act as transcription factors; 60-100 amino acids long. A DNA-binding basic region is followed by two alpha-helices separated by a variable loop region; HLH forms homo- and heterodimers, ...

cd00130
Location:315 → 415

PAS; PAS domain; PAS motifs appear in archaea, eubacteria and eukarya. Probably the most surprising identification of a PAS domain was that in EAG-like K+-channels. PAS domains have been found to bind ligands, and to act as sensors for light and oxygen in ...

pfam13426
Location:159 → 260

PAS_9; PAS domain

pfam14598
Location:314 → 417

PAS_11; PAS domain
XM_005263961.4 → XP_005264018.1 neuronal PAS domain-containing protein 2 isoform X9

Conserved Domains (2) summary

cd00130
Location:126 → 226

PAS; PAS domain; PAS motifs appear in archaea, eubacteria and eukarya. Probably the most surprising identification of a PAS domain was that in EAG-like K+-channels. PAS domains have been found to bind ligands, and to act as sensors for light and oxygen in ...

pfam14598
Location:125 → 228

PAS_11; PAS domain