Format

Send to:

Choose Destination

ALOX5 arachidonate 5-lipoxygenase [ Homo sapiens (human) ]

Gene ID: 240, updated on 17-Jun-2019

Summary

Official Symbol
ALOX5provided by HGNC
Official Full Name
arachidonate 5-lipoxygenaseprovided by HGNC
Primary source
HGNC:HGNC:435
See related
Ensembl:ENSG00000012779 MIM:152390
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
5-LO; 5LPG; LOG5; 5-LOX
Summary
This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Expression
Broad expression in bone marrow (RPKM 35.1), lung (RPKM 26.8) and 15 other tissues See more
Orthologs

Genomic context

See ALOX5 in Genome Data Viewer
Location:
10q11.21
Exon count:
13
Annotation release Status Assembly Chr Location
109.20190607 current GRCh38.p13 (GCF_000001405.39) 10 NC_000010.11 (45374166..45446121)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 10 NC_000010.10 (45869624..45941569)

Chromosome 10 - NC_000010.11Genomic Context describing neighboring genes Neighboring gene olfactory receptor family 6 subfamily D member 1 pseudogene Neighboring gene olfactory receptor family 13 subfamily A member 1 Neighboring gene uncharacterized LOC102724323 Neighboring gene uncharacterized LOC105378286 Neighboring gene membrane associated ring-CH-type finger 8 Neighboring gene VISTA enhancer hs2065

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

Phenotypes

Associated conditions

Description Tests
Asthma, susceptibility to
MedGen: C1869116 OMIM: 600807 GeneReviews: Not available
not available

NHGRI GWAS Catalog

Description
Discovery and refinement of loci associated with lipid levels.
NHGRI GWA Catalog
Genome-wide association study of hematological and biochemical traits in a Japanese population.
NHGRI GWA Catalog

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env HIV-1 gp120 downregulates the expression of arachidonate 5-lipoxygenase (ALOX5) in human B cells PubMed
env HIV-1 gp120 increases the intracellular concentrations of prostaglandin E2 and leukotriene B4 by upregulating the activity and expression of the arachidonate-metabolizing enzymes prostaglandin H synthase and 5-lipoxygenase, respectively PubMed

Go to the HIV-1, Human Interaction Database

Pathways from BioSystems

  • Arachidonic acid metabolism, organism-specific biosystem (from KEGG)
    Arachidonic acid metabolism, organism-specific biosystem
    Arachidonic acid metabolism
  • Arachidonic acid metabolism, organism-specific biosystem (from REACTOME)
    Arachidonic acid metabolism, organism-specific biosystemEicosanoids, oxygenated, 20-carbon fatty acids, are autocrine and paracrine signaling molecules that modulate physiological processes including pain, fever, inflammation, blood clot formation, smooth...
  • Arachidonic acid metabolism, conserved biosystem (from KEGG)
    Arachidonic acid metabolism, conserved biosystem
    Arachidonic acid metabolism
  • Cytokine Signaling in Immune system, organism-specific biosystem (from REACTOME)
    Cytokine Signaling in Immune system, organism-specific biosystemCytokines are small proteins that regulate and mediate immunity, inflammation, and hematopoiesis. They are secreted in response to immune stimuli, and usually act briefly, locally, at very low concen...
  • Eicosanoid Synthesis, organism-specific biosystem (from WikiPathways)
    Eicosanoid Synthesis, organism-specific biosystemIn biochemistry, eicosanoids are signaling molecules made by oxidation of twenty-carbon essential fatty acids, (EFAs). They exert complex control over many bodily systems, mainly in inflammation or i...
  • Fc epsilon RI signaling pathway, organism-specific biosystem (from KEGG)
    Fc epsilon RI signaling pathway, organism-specific biosystemFc epsilon RI-mediated signaling pathways in mast cells are initiated by the interaction of antigen (Ag) with IgE bound to the extracellular domain of the alpha chain of Fc epsilon RI. The activation...
  • Fc epsilon RI signaling pathway, conserved biosystem (from KEGG)
    Fc epsilon RI signaling pathway, conserved biosystemFc epsilon RI-mediated signaling pathways in mast cells are initiated by the interaction of antigen (Ag) with IgE bound to the extracellular domain of the alpha chain of Fc epsilon RI. The activation...
  • Immune System, organism-specific biosystem (from REACTOME)
    Immune System, organism-specific biosystemHumans are exposed to millions of potential pathogens daily, through contact, ingestion, and inhalation. Our ability to avoid infection depends on the adaptive immune system and during the first crit...
  • Innate Immune System, organism-specific biosystem (from REACTOME)
    Innate Immune System, organism-specific biosystemInnate immunity encompases the nonspecific part of immunity tha are part of an individual's natural biologic makeup
  • Interleukin-4 and 13 signaling, organism-specific biosystem (from REACTOME)
    Interleukin-4 and 13 signaling, organism-specific biosystemInterleukin-4 (IL4) is a principal regulatory cytokine during the immune response, crucially important in allergy and asthma (Nelms et al. 1999). When resting T cells are antigen-activated and expand...
  • Metabolic pathways, organism-specific biosystem (from KEGG)
    Metabolic pathways, organism-specific biosystem
    Metabolic pathways
  • Metabolism, organism-specific biosystem (from REACTOME)
    Metabolism, organism-specific biosystemMetabolic processes in human cells generate energy through the oxidation of molecules consumed in the diet and mediate the synthesis of diverse essential molecules not taken in the diet as well as th...
  • Metabolism of lipids and lipoproteins, organism-specific biosystem (from REACTOME)
    Metabolism of lipids and lipoproteins, organism-specific biosystemLipids are hydrophobic but otherwise chemically diverse molecules that play a wide variety of roles in human biology. They include ketone bodies, fatty acids, triacylglycerols, phospholipids and sphi...
  • Neutrophil degranulation, organism-specific biosystem (from REACTOME)
    Neutrophil degranulation, organism-specific biosystemNeutrophils are the most abundant leukocytes (white blood cells), indispensable in defending the body against invading microorganisms. In response to infection, neutrophils leave the circulation and ...
  • Ovarian steroidogenesis, organism-specific biosystem (from KEGG)
    Ovarian steroidogenesis, organism-specific biosystemThe ovarian steroids, 17-beta estradiol (E2) and progesterone (P4), are critical for normal uterine function, establishment and maintenance of pregnancy, and mammary gland development. Furthermore, t...
  • Ovarian steroidogenesis, conserved biosystem (from KEGG)
    Ovarian steroidogenesis, conserved biosystemThe ovarian steroids, 17-beta estradiol (E2) and progesterone (P4), are critical for normal uterine function, establishment and maintenance of pregnancy, and mammary gland development. Furthermore, t...
  • Selenium Micronutrient Network, organism-specific biosystem (from WikiPathways)
    Selenium Micronutrient Network, organism-specific biosystem
    Selenium Micronutrient Network
  • Serotonergic synapse, organism-specific biosystem (from KEGG)
    Serotonergic synapse, organism-specific biosystemSerotonin (5-Hydroxytryptamine, 5-HT) is a monoamine neurotransmitter that plays important roles in physiological functions such as learning and memory, emotion, sleep, pain, motor function and endoc...
  • Signaling by Interleukins, organism-specific biosystem (from REACTOME)
    Signaling by Interleukins, organism-specific biosystemInterleukins are low molecular weight proteins that bind to cell surface receptors and act in an autocrine and/or paracrine fashion. They were first identified as factors produced by leukocytes but a...
  • Synthesis of 5-eicosatetraenoic acids, organism-specific biosystem (from REACTOME)
    Synthesis of 5-eicosatetraenoic acids, organism-specific biosystem5-hydroperoxy-eicosatetraenoic acid (5-HpETE), 5-hydroxyeicosatetraenoic acid (5S-HETE) and 5-oxo-eicosatetraenoic acid (5-oxoETE) are formed after the initial step of arachidonic acid oxidation by ...
  • Synthesis of Leukotrienes (LT) and Eoxins (EX), organism-specific biosystem (from REACTOME)
    Synthesis of Leukotrienes (LT) and Eoxins (EX), organism-specific biosystemLeukotrienes (LTs) are biologically active molecules formed in response to inflammatory stimuli. They cause contraction of bronchial smooth muscles, stimulation of vascular permeability, and attracti...
  • Synthesis of Lipoxins (LX), organism-specific biosystem (from REACTOME)
    Synthesis of Lipoxins (LX), organism-specific biosystemLipoxins A4 (LXA4) and B4 (LXB4), structurally characterized from human neutrophils incubated with 15-hydroperoxy-eicosatetraenoic acid (15-HpETE), each contain three hydroxyl moieties and a conjugat...
  • Toxoplasmosis, organism-specific biosystem (from KEGG)
    Toxoplasmosis, organism-specific biosystemToxoplasma gondii is an obligate intracellular parasite that is prevalent worldwide. The tachyzoite form acquired by oral ingestion downmodulates proinflammatory signaling pathways via various mechan...
  • Toxoplasmosis, conserved biosystem (from KEGG)
    Toxoplasmosis, conserved biosystemToxoplasma gondii is an obligate intracellular parasite that is prevalent worldwide. The tachyzoite form acquired by oral ingestion downmodulates proinflammatory signaling pathways via various mechan...
  • aspirin triggered resolvin D biosynthesis, organism-specific biosystem (from BIOCYC)
    aspirin triggered resolvin D biosynthesis, organism-specific biosystemGeneral Background The ω 3 polyunsaturated fatty acids are known to be essential for proper neuronal and immune function. Deficiencies in these important fatty acids are associated with mental...
  • aspirin triggered resolvin D biosynthesis, conserved biosystem (from BIOCYC)
    aspirin triggered resolvin D biosynthesis, conserved biosystemGeneral Background The ω 3 polyunsaturated fatty acids are known to be essential for proper neuronal and immune function. Deficiencies in these important fatty acids are associated with mental...
  • aspirin triggered resolvin E biosynthesis, organism-specific biosystem (from BIOCYC)
    aspirin triggered resolvin E biosynthesis, organism-specific biosystemGeneral Background Resolvins (resolution phase interaction products) are a new class of anti-inflammatory mediators derived from the ω 3 fatty acids eicosapentaenoate and docosahexaenoate. Tho...
  • aspirin triggered resolvin E biosynthesis, conserved biosystem (from BIOCYC)
    aspirin triggered resolvin E biosynthesis, conserved biosystemGeneral Background Resolvins (resolution phase interaction products) are a new class of anti-inflammatory mediators derived from the ω 3 fatty acids eicosapentaenoate and docosahexaenoate. Tho...
  • aspirin-triggered lipoxin biosynthesis, organism-specific biosystem (from BIOCYC)
    aspirin-triggered lipoxin biosynthesis, organism-specific biosystemGeneral Background Lipoxins belong to the eicosanoid family, have a distinct conjugated tetraene structure and are generated from : ARACHIDONIC_ACID. These lipid derived mediators play an important ...
  • aspirin-triggered lipoxin biosynthesis, conserved biosystem (from BIOCYC)
    aspirin-triggered lipoxin biosynthesis, conserved biosystemGeneral Background Lipoxins belong to the eicosanoid family, have a distinct conjugated tetraene structure and are generated from |FRAME: ARACHIDONIC_ACID|. These lipid derived mediators play an imp...
  • leukotriene biosynthesis, organism-specific biosystem (from BIOCYC)
    leukotriene biosynthesis, organism-specific biosystemLeukotrienes are made by cells of the immune system, mainly the mediators of inflammation like polymorphonuclear leukocytes, macrophages and mast cells. Cellular activation during inflammation leads ...
  • leukotriene biosynthesis, conserved biosystem (from BIOCYC)
    leukotriene biosynthesis, conserved biosystemLeukotrienes are proinflammatory metabolites of arachidonic acid that activate and amplify innate and adaptive immune responses. They also have roles in pathologic immune responses, mediating contrac...
  • lipoxin biosynthesis, organism-specific biosystem (from BIOCYC)
    lipoxin biosynthesis, organism-specific biosystemGeneral Background Lipoxins belong to the eicosanoid family, have a distinct conjugated tetraene structure and are generated from : ARACHIDONIC_ACID during inflammation. They consist of two distinct...
  • lipoxin biosynthesis, conserved biosystem (from BIOCYC)
    lipoxin biosynthesis, conserved biosystemGeneral Background Lipoxins belong to the eicosanoid family, have a distinct conjugated tetraene structure and are generated from |FRAME: ARACHIDONIC_ACID| during inflammation. They consist of two d...
  • resolvin D biosynthesis, organism-specific biosystem (from BIOCYC)
    resolvin D biosynthesis, organism-specific biosystemGeneral Background The ω 3 polyunsaturated fatty acids are known to be essential for proper neuronal and immune function. Deficiencies in these important fatty acids are associated with mental...
  • resolvin D biosynthesis, conserved biosystem (from BIOCYC)
    resolvin D biosynthesis, conserved biosystemGeneral Background The ω 3 polyunsaturated fatty acids are essential for proper neuronal and immune function. Deficiencies in these important fatty acids are associated with mental dysfunction...

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • MGC163204

Gene Ontology Provided by GOA

Function Evidence Code Pubs
arachidonate 5-lipoxygenase activity IDA
Inferred from Direct Assay
more info
PubMed 
arachidonate 5-lipoxygenase activity TAS
Traceable Author Statement
more info
 
iron ion binding IDA
Inferred from Direct Assay
more info
PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
Process Evidence Code Pubs
cytokine-mediated signaling pathway TAS
Traceable Author Statement
more info
 
interleukin-18-mediated signaling pathway TAS
Traceable Author Statement
more info
 
leukotriene biosynthetic process IDA
Inferred from Direct Assay
more info
PubMed 
leukotriene metabolic process TAS
Traceable Author Statement
more info
 
leukotriene production involved in inflammatory response IEA
Inferred from Electronic Annotation
more info
 
lipoxin biosynthetic process TAS
Traceable Author Statement
more info
 
lipoxygenase pathway TAS
Traceable Author Statement
more info
 
long-chain fatty acid biosynthetic process TAS
Traceable Author Statement
more info
 
neutrophil degranulation TAS
Traceable Author Statement
more info
 
oxidation-reduction process IEA
Inferred from Electronic Annotation
more info
 
Component Evidence Code Pubs
cytosol IDA
Inferred from Direct Assay
more info
PubMed 
cytosol TAS
Traceable Author Statement
more info
 
extracellular region TAS
Traceable Author Statement
more info
 
extracellular space HDA PubMed 
ficolin-1-rich granule lumen TAS
Traceable Author Statement
more info
 
nuclear envelope IDA
Inferred from Direct Assay
more info
PubMed 
nuclear envelope TAS
Traceable Author Statement
more info
 
nuclear envelope lumen IDA
Inferred from Direct Assay
more info
PubMed 
nuclear matrix IDA
Inferred from Direct Assay
more info
PubMed 
nuclear membrane IDA
Inferred from Direct Assay
more info
PubMed 
nucleoplasm IDA
Inferred from Direct Assay
more info
 
secretory granule lumen TAS
Traceable Author Statement
more info
 

General protein information

Preferred Names
arachidonate 5-lipoxygenase
Names
LOX-5
arachidonic 5-lipoxygenase alpha-10 isoform
arachidonic 5-lipoxygenase delta-10-13 isoform
arachidonic 5-lipoxygenase delta-13 isoform
arachidonic 5-lipoxygenase delta-p10 isoform
arachidonic acid 5-lipoxygenase
leukotriene A4 synthase
NP_000689.1
NP_001243082.1
NP_001243083.1
NP_001307790.1
NP_001307791.1
XP_016871501.1

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_011437.1 RefSeqGene

    Range
    4986..76939
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_000698.5NP_000689.1  arachidonate 5-lipoxygenase isoform 1

    See identical proteins and their annotated locations for NP_000689.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes the longest isoform (1).
    Source sequence(s)
    AW403395, BC132677
    Consensus CDS
    CCDS7212.1
    UniProtKB/Swiss-Prot
    P09917
    Related
    ENSP00000363512.2, ENST00000374391.7
    Conserved Domains (2) summary
    cd01753
    Location:2115
    PLAT_LOX; PLAT domain of 12/15-lipoxygenase. As a unique subfamily of the mammalian lipoxygenases, they catalyze enzymatic lipid peroxidation in complex biological structures via direct dioxygenation of phospholipids and cholesterol esters of biomembranes and ...
    cl27717
    Location:227665
    Lipoxygenase; Lipoxygenase
  2. NM_001256153.3NP_001243082.1  arachidonate 5-lipoxygenase isoform 2

    See identical proteins and their annotated locations for NP_001243082.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) lacks an in-frame segment in the coding region, compared to variant 1. The resulting isoform (2) is shorter than isoform 1.
    Source sequence(s)
    AK225603, AK315223, AW403395, BC132677
    UniProtKB/Swiss-Prot
    P09917
    Conserved Domains (2) summary
    cd01753
    Location:2115
    PLAT_LOX; PLAT domain of 12/15-lipoxygenase. As a unique subfamily of the mammalian lipoxygenases, they catalyze enzymatic lipid peroxidation in complex biological structures via direct dioxygenation of phospholipids and cholesterol esters of biomembranes and ...
    pfam00305
    Location:227633
    Lipoxygenase; Lipoxygenase
  3. NM_001256154.2NP_001243083.1  arachidonate 5-lipoxygenase isoform 3

    See identical proteins and their annotated locations for NP_001243083.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) lacks an in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (3) is shorter than isoform 1.
    Source sequence(s)
    AW403395, BC143985, HY102907
    Consensus CDS
    CCDS58078.1
    UniProtKB/Swiss-Prot
    P09917
    Related
    ENSP00000437634.1, ENST00000542434.5
    Conserved Domains (2) summary
    cd01753
    Location:2115
    PLAT_LOX; PLAT domain of 12/15-lipoxygenase. As a unique subfamily of the mammalian lipoxygenases, they catalyze enzymatic lipid peroxidation in complex biological structures via direct dioxygenation of phospholipids and cholesterol esters of biomembranes and ...
    pfam00305
    Location:227608
    Lipoxygenase; Lipoxygenase
  4. NM_001320861.2NP_001307790.1  arachidonate 5-lipoxygenase isoform 4

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. The encoded isoform (4) is shorter than isoform 1.
    Source sequence(s)
    AL731567, AW403395, BC132677
    UniProtKB/Swiss-Prot
    P09917
    Conserved Domains (2) summary
    cd01753
    Location:2115
    PLAT_LOX; PLAT domain of 12/15-lipoxygenase. As a unique subfamily of the mammalian lipoxygenases, they catalyze enzymatic lipid peroxidation in complex biological structures via direct dioxygenation of phospholipids and cholesterol esters of biomembranes and ...
    pfam00305
    Location:227636
    Lipoxygenase; Lipoxygenase
  5. NM_001320862.2NP_001307791.1  arachidonate 5-lipoxygenase isoform 5

    Status: REVIEWED

    Description
    Transcript Variant: This variant (5) differs in the 5' UTR and initiates translation at a downstream start site compared to variant 1. The encoded isoform (5) has a shorter N-terminus than isoform 1.
    Source sequence(s)
    AW403395, BC143986
    Conserved Domains (1) summary
    pfam00305
    Location:82520
    Lipoxygenase; Lipoxygenase

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000010.11 Reference GRCh38.p13 Primary Assembly

    Range
    45374166..45446121
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017016012.1XP_016871501.1  arachidonate 5-lipoxygenase isoform X1

    Conserved Domains (1) summary
    pfam00305
    Location:82520
    Lipoxygenase; Lipoxygenase

RNA

  1. XR_001747075.1 RNA Sequence

Reference GRCh38.p13 ALT_REF_LOCI_1

Genomic

  1. NW_003315935.1 Reference GRCh38.p13 ALT_REF_LOCI_1

    Range
    214997..286950
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Support Center