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Science. 2011 Jan 14;331(6014):217-9. doi: 10.1126/science.1197203.

The structure of human 5-lipoxygenase.

Author information

1
Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.

Abstract

The synthesis of both proinflammatory leukotrienes and anti-inflammatory lipoxins requires the enzyme 5-lipoxygenase (5-LOX). 5-LOX activity is short-lived, apparently in part because of an intrinsic instability of the enzyme. We identified a 5-LOX-specific destabilizing sequence that is involved in orienting the carboxyl terminus, which binds the catalytic iron. Here, we report the crystal structure at 2.4 angstrom resolution of human 5-LOX stabilized by replacement of this sequence.

PMID:
21233389
PMCID:
PMC3245680
DOI:
10.1126/science.1197203
[Indexed for MEDLINE]
Free PMC Article

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