Prkce protein kinase C, epsilon [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Prkceprovided by MGI
Official Full Name: protein kinase C, epsilonprovided by MGI
Primary source: MGI:MGI:97599
See related: Ensembl:ENSMUSG00000045038 AllianceGenome:MGI:97599
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Pkce; PKC[e]; PKCepsilon; 5830406C15Rik
Summary: Enables several functions, including 14-3-3 protein binding activity; actin monomer binding activity; and diacylglycerol-dependent, calcium-independent serine/threonine kinase activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of actin filament polymerization; and positive regulation of fibroblast migration. Acts upstream of or within several processes, including cellular response to alcohol; positive regulation of cell communication; and regulation of secretion. Located in several cellular components, including cytosol; mitochondrion; and perinuclear region of cytoplasm. Colocalizes with Golgi apparatus. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; heart; and submandibular gland primordium. Orthologous to human PRKCE (protein kinase C epsilon). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in frontal lobe adult (RPKM 22.3), cortex adult (RPKM 20.6) and 19 other tissues See more
Orthologs: human all
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Genomic context

Location:: 17 E4; 17 56.74 cM

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (86472631..86965347)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (86167785..86657919)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The binding of PKCepsilon and MEG2 to STAT3 regulates IL-6-mediated microglial hyperalgesia during inflammatory pain.
Title: The binding of PKCε and MEG2 to STAT3 regulates IL-6-mediated microglial hyperalgesia during inflammatory pain.
Brain-specific serine/threonine-protein kinase 1 is a substrate of protein kinase C epsilon involved in sex-specific ethanol and anxiety phenotypes.
Title: Brain-specific serine/threonine-protein kinase 1 is a substrate of protein kinase C epsilon involved in sex-specific ethanol and anxiety phenotypes.
Hepatocyte NLRP3 interacts with PKCepsilon to drive hepatic insulin resistance and steatosis.
Title: Hepatocyte NLRP3 interacts with PKCε to drive hepatic insulin resistance and steatosis.
cAMP-EPAC-PKCepsilon-RIM1alpha signaling regulates presynaptic long-term potentiation and motor learning.
Title: cAMP-EPAC-PKCε-RIM1α signaling regulates presynaptic long-term potentiation and motor learning.
Chemical Genetic Identification of PKC Epsilon Substrates in Mouse Brain.
Title: Chemical Genetic Identification of PKC Epsilon Substrates in Mouse Brain.
Distinct subcellular localisation of intramyocellular lipids and reduced PKCepsilon/PKCtheta; activity preserve muscle insulin sensitivity in exercise-trained mice.
Title: Distinct subcellular localisation of intramyocellular lipids and reduced PKCε/PKCθ activity preserve muscle insulin sensitivity in exercise-trained mice.
Protein kinase C epsilon promotes de novo lipogenesis and tumor growth in prostate cancer cells by regulating the phosphorylation and nuclear translocation of pyruvate kinase isoform M2.
Title: Protein kinase C epsilon promotes de novo lipogenesis and tumor growth in prostate cancer cells by regulating the phosphorylation and nuclear translocation of pyruvate kinase isoform M2.
Maternal Prkce expression in mature oocytes is critical for the first cleavage facilitating maternal-to-zygotic transition in mouse early embryos.
Title: Maternal Prkce expression in mature oocytes is critical for the first cleavage facilitating maternal-to-zygotic transition in mouse early embryos.
Activation of PKCepsilon-ALDH2 Axis Prevents 4-HNE-Induced Pain in Mice.
Title: Activation of PKCε-ALDH2 Axis Prevents 4-HNE-Induced Pain in Mice.
Bidirectional sex-dependent regulation of alpha6 and beta3 nicotinic acetylcholine receptors by protein kinase Cepsilon.
Title: Bidirectional sex-dependent regulation of α6 and β3 nicotinic acetylcholine receptors by protein kinase Cε.

Submit: New GeneRIF Correction See all GeneRIFs (164)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (1)
Endonuclease-mediated (5)
Targeted (6) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AW047481 (e-PCR)
- UniSTS:181076

RH125176 (e-PCR)
- UniSTS:161998

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 14-3-3 protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables SH3 domain binding	ISO Inferred from Sequence Orthology more info
enables actin monomer binding	IDA Inferred from Direct Assay more info	PubMed
enables diacylglycerol-dependent serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables diacylglycerol-dependent, calcium-independent serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables enzyme activator activity	IEA Inferred from Electronic Annotation more info
enables enzyme activator activity	ISO Inferred from Sequence Orthology more info
enables enzyme binding	IEA Inferred from Electronic Annotation more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables ethanol binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein kinase binding	ISO Inferred from Sequence Orthology more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase binding	ISO Inferred from Sequence Orthology more info
enables signaling receptor activator activity	IDA Inferred from Direct Assay more info	PubMed
enables signaling receptor binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of MAPK cascade	IDA Inferred from Direct Assay more info	PubMed
involved_in TRAM-dependent toll-like receptor 4 signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell division	IEA Inferred from Electronic Annotation more info
acts_upstream_of cell-substrate adhesion	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of cell-substrate adhesion	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cellular response to ethanol	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within cellular response to hypoxia	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to platelet-derived growth factor stimulus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to prostaglandin E stimulus	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within chemosensory behavior	TAS Traceable Author Statement more info	PubMed
acts_upstream_of establishment of localization in cell	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of insulin secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular signal transduction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within lipopolysaccharide-mediated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipopolysaccharide-mediated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within locomotory exploration behavior	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within macrophage activation involved in immune response	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of mucus secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of mitochondrial calcium ion concentration	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of mitochondrial membrane potential	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of protein ubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of release of sequestered calcium ion into cytosol	ISO Inferred from Sequence Orthology more info
involved_in peptidyl-serine phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in peptidyl-serine phosphorylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of MAPK cascade	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of MAPK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of actin filament polymerization	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of canonical NF-kappaB signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of cell-substrate adhesion	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within positive regulation of cell-substrate adhesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cellular glucuronidation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cellular glucuronidation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cytokinesis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cytokinesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of epithelial cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of epithelial cell migration	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of fibroblast migration	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of insulin secretion	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of lipid catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of mucus secretion	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of synaptic transmission, GABAergic	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of wound healing	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of wound healing	ISS Inferred from Sequence or Structural Similarity more info
involved_in presynaptic modulation of chemical synaptic transmission	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of insulin secretion involved in cellular response to glucose stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of lipid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of peptidyl-tyrosine phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of release of sequestered calcium ion into cytosol	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of release of sequestered calcium ion into cytosol	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to morphine	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of synaptic transmission, GABAergic	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in toxin catabolic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
colocalizes_with Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi membrane	ISO Inferred from Sequence Orthology more info
located_in T-tubule	ISO Inferred from Sequence Orthology more info
located_in cell periphery	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in intermediate filament cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in intermediate filament cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
is_active_in neuromuscular junction	ISO Inferred from Sequence Orthology more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in presynaptic cytosol	ISO Inferred from Sequence Orthology more info
located_in synapse	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: protein kinase C epsilon type

Names: PCK epsilon; nPKC-epsilon

NP_035234.1

EC 2.7.11.13

XP_006523896.1

EC 2.7.11.13

XP_017172817.1

EC 2.7.11.13

XP_030105436.1

EC 2.7.11.13

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_011104.3 → NP_035234.1 protein kinase C epsilon type

See identical proteins and their annotated locations for NP_035234.1

Status: VALIDATED

Source sequence(s)

AC096777, AC154236, AC154351, AC154542, CT030001

Consensus CDS

CCDS29008.1

UniProtKB/Swiss-Prot

P16054

Related

ENSMUSP00000094874.3, ENSMUST00000097275.9

Conserved Domains (4) summary

cd04014
Location:3 → 135

C2_PKC_epsilon; C2 domain in Protein Kinase C (PKC) epsilon

smart00220
Location:408 → 668

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd05591
Location:412 → 732

STKc_nPKC_epsilon; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C epsilon

pfam00130
Location:243 → 295

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000083.7 Reference GRCm39 C57BL/6J

Range

86472631..86965347

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017317328.3 → XP_017172817.1 protein kinase C epsilon type isoform X1

Conserved Domains (3) summary

cd04014
Location:3 → 135

C2_PKC_epsilon; C2 domain in Protein Kinase C (PKC) epsilon

pfam00130
Location:243 → 293

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

cl21453
Location:412 → 689

PKc_like; Protein Kinases, catalytic domain
XM_030249576.2 → XP_030105436.1 protein kinase C epsilon type isoform X3

Conserved Domains (2) summary

cd05591
Location:262 → 582

STKc_nPKC_epsilon; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C epsilon

pfam00130
Location:93 → 143

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
XM_006523833.3 → XP_006523896.1 protein kinase C epsilon type isoform X2

Conserved Domains (2) summary

cd05591
Location:282 → 602

STKc_nPKC_epsilon; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C epsilon

pfam00130
Location:113 → 163

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)