Dapk3 death-associated protein kinase 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Dapk3provided by MGI
Official Full Name: death-associated protein kinase 3provided by MGI
Primary source: MGI:MGI:1203520
See related: Ensembl:ENSMUSG00000034974 AllianceGenome:MGI:1203520
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: dlk; ZIPK
Summary: Enables ATP binding activity and protein serine/threonine kinase activity. Involved in several processes, including cellular response to interferon-gamma; regulation of cell shape; and regulation of myosin II filament organization. Acts upstream of or within apoptotic signaling pathway. Located in PML body. Is expressed in heart; mammary gland epithelium; and notochord. Orthologous to human DAPK3 (death associated protein kinase 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in ovary adult (RPKM 67.6), duodenum adult (RPKM 59.4) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 10 C1; 10 39.72 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
109	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (81018821..81029031)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (81182987..81193197)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The tumor suppressor kinase DAPK3 drives tumor-intrinsic immunity through the STING-IFN-beta pathway.
Title: The tumor suppressor kinase DAPK3 drives tumor-intrinsic immunity through the STING-IFN-β pathway.
The induced deletion of Zipk significantly inhibited ischemia-reperfusion-induced blood-brain barrier dysfunction and neuronal injuries from middle cerebral artery occlusion and reperfusion, as evidenced by reduced infarct and edema volume, attenuated Evans blue dye leakage, and improved neuronal behavior.
Title: ZIPK mediates endothelial cell contraction through myosin light chain phosphorylation and is required for ischemic-reperfusion injury.
Cellular and mouse modeling studies show that DAPK3 is a tumor suppressor gene and is important in early development.
Title: DAPK3 suppresses acini morphogenesis and is required for mouse development.
DAPK3 plays a central role in preventing miR-17/miR-20a depletion-induced genome instability and in miR-17/miR-20a overexpression-triggered tumor formation.
Title: Oncogenic miR-17/20a Forms a Positive Feed-forward Loop with the p53 Kinase DAPK3 to Promote Tumorigenesis.
The C-terminal domain (NLS2 motif)of ZIPK of murine is nonfunctional for transport.
Title: Phosphorylation-dependent control of ZIPK nuclear import is species specific.
ZIPK may act as a cardiac RLC kinase and thereby affect contractility.
Title: Cardiac myosin is a substrate for zipper-interacting protein kinase (ZIPK).
murine ZIPK underwent specific divergence from a conserved consensus
Title: ZIPK: a unique case of murine-specific divergence of a conserved vertebrate gene.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (1)
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH127807 (e-PCR)
- UniSTS:211117

NoName (e-PCR)
- UniSTS:491222

Homology

Homologs of the Dapk3 gene: The Dapk3 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, rat, chicken, zebrafish, and frog.
Orthologs from Annotation Pipeline: 458 organisms have orthologs with human gene Dapk3
Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables kinase activity	ISO Inferred from Sequence Orthology more info	PubMed
enables leucine zipper domain binding	ISO Inferred from Sequence Orthology more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables small GTPase binding	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within apoptotic signaling pathway	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within apoptotic signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to type II interferon	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to type II interferon	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within chromatin organization	IEA Inferred from Electronic Annotation more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in intracellular signal transduction	IDA Inferred from Direct Assay more info	PubMed
involved_in intracellular signal transduction	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of translation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of apoptotic process	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in positive regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of canonical Wnt signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of extrinsic apoptotic signaling pathway in absence of ligand	ISO Inferred from Sequence Orthology more info
involved_in protein autophosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of actin cytoskeleton reorganization	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell shape	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of focal adhesion assembly	ISO Inferred from Sequence Orthology more info
involved_in regulation of mitotic cell cycle	ISO Inferred from Sequence Orthology more info
involved_in regulation of myosin II filament organization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in PML body	IDA Inferred from Direct Assay more info	PubMed
located_in actin filament	ISO Inferred from Sequence Orthology more info
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, centromeric region	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in membrane raft	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: death-associated protein kinase 3

Names: DAP kinase 3; DAP-like kinase; MYPT1 kinase; ZIP-kinase; death-associated kinase 3

NP_001177402.1

EC 2.7.11.1

NP_001177403.2

EC 2.7.11.1

NP_031854.1

EC 2.7.11.1

XP_006513257.1

EC 2.7.11.1

XP_006513258.1

EC 2.7.11.1

XP_030100726.1

EC 2.7.11.1

XP_036011469.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001190473.1 → NP_001177402.1 death-associated protein kinase 3 isoform b

See identical proteins and their annotated locations for NP_001177402.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2 and 3 both encode the same isoform (b).

Source sequence(s)

AC155932

Consensus CDS

CCDS24045.1

UniProtKB/Swiss-Prot

O54784

UniProtKB/TrEMBL

A0A6P5QA61, Q05A21

Related

ENSMUSP00000035962.7, ENSMUST00000047665.7

Conserved Domains (1) summary

cd14105
Location:7 → 275

STKc_DAPK; Catalytic domain of the Serine/Threonine Kinase, Death-Associated Protein Kinase
NM_001190474.1 → NP_001177403.2 death-associated protein kinase 3 isoform a

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a).

Source sequence(s)

AC155932

UniProtKB/TrEMBL

A0A8C6I065

Conserved Domains (2) summary

smart00220
Location:30 → 292

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14105
Location:24 → 292

STKc_DAPK; Catalytic domain of the Serine/Threonine Kinase, Death-Associated Protein Kinase
NM_007828.2 → NP_031854.1 death-associated protein kinase 3 isoform b

See identical proteins and their annotated locations for NP_031854.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2 and 3 both encode the same isoform (b).

Source sequence(s)

AC155932

Consensus CDS

CCDS24045.1

UniProtKB/Swiss-Prot

O54784

UniProtKB/TrEMBL

A0A6P5QA61, Q05A21

Related

ENSMUSP00000151577.2, ENSMUST00000219850.2

Conserved Domains (1) summary

cd14105
Location:7 → 275

STKc_DAPK; Catalytic domain of the Serine/Threonine Kinase, Death-Associated Protein Kinase

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000076.7 Reference GRCm39 C57BL/6J

Range

81018821..81029031

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006513194.2 → XP_006513257.1 death-associated protein kinase 3 isoform X1

Conserved Domains (1) summary

cl21453
Location:24 → 285

PKc_like; Protein Kinases, catalytic domain
XM_030244866.1 → XP_030100726.1 death-associated protein kinase 3 isoform X3

Conserved Domains (1) summary

cl21453
Location:7 → 268

PKc_like; Protein Kinases, catalytic domain
XM_006513195.4 → XP_006513258.1 death-associated protein kinase 3 isoform X2

See identical proteins and their annotated locations for XP_006513258.1

UniProtKB/Swiss-Prot

O54784

UniProtKB/TrEMBL

A0A6P5QA61, Q05A21

Conserved Domains (1) summary

cd14105
Location:7 → 275

STKc_DAPK; Catalytic domain of the Serine/Threonine Kinase, Death-Associated Protein Kinase
XM_036155576.1 → XP_036011469.1 death-associated protein kinase 3 isoform X3

Conserved Domains (1) summary

cl21453
Location:7 → 268

PKc_like; Protein Kinases, catalytic domain