|
| Status |
Public on Jul 19, 2017 |
| Title |
oxBS-MAC20 D3 |
| Sample type |
genomic |
| |
|
| Source name |
MAC20_donor 3 (oxidative bisulfite-treated, oxBS)
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: CD14 cells treated with M-CSF for 20 days
|
| Treatment protocol |
Bisulfite (BS) samples obtained by standard bisulfite modification. Oxidative-bisulfite (oxBS)-derived samples were obtained after potassium perruthenate treatment and later bisulfite modification
|
| Growth protocol |
Human monocytes were isolated using MACS CD14 microbeads (Milteny) from peripheral blood mononuclear cells using the manufacturer's protocol
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Not provided
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
BS or oxBS converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450k Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader (Illumina HiSeq2000) using standard recommended Illumina scanner setting
|
| Description |
CD14 cells treated with M-CSF for 20 days (oxidative bisulfite-treated, oxBS)
|
| Data processing |
Regular BeadStudio processing
|
| |
|
| Submission date |
Apr 07, 2017 |
| Last update date |
Jul 19, 2017 |
| Contact name |
Esteban Ballestar |
| Organization name |
Josep Carreras Research Institute (IJC)
|
| Lab |
Epigenetics and Immune Disease
|
| Street address |
Ctra de Can Ruti, Camà de les Escoles s/n
|
| City |
Badalona, Barcelona |
| State/province |
N/A = Not Applicable |
| ZIP/Postal code |
08916 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE97497 |
Comparison of the DNA methylation profiles of CD14+ monocytes from human peripheral blood with derived macrophages (MACs) and osteoclasts (OCs) obtained by exposure with M-CSF and M-CSF/RANKL, respectively, for 5 and 20 days |
|
