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Series GSE97497 Query DataSets for GSE97497
Status Public on Jul 19, 2017
Title Comparison of the DNA methylation profiles of CD14+ monocytes from human peripheral blood with derived macrophages (MACs) and osteoclasts (OCs) obtained by exposure with M-CSF and M-CSF/RANKL, respectively, for 5 and 20 days
Organism Homo sapiens
Experiment type Methylation profiling by genome tiling array
Summary The methylation profiles of bisulfite(BS)- and oxidative-BS-modified DNA of human CD14+ monocytes were compared with derived macrophages (MACs) and osteoclasts (OCs) following M-CSF and M-CSF/RANKL incubation for 5 and 20 days, using the Infinium HumanMethylation450 BeadChips (Illumina, Inc., San Diego, CA,). This platform allows the interrogation of >485,000 methylation sites per sample at single-nucleotide resolution, and comprises an average of 17 CpG sites per gene in the 99% of RefSeq genes. 96% of CpG islands are covered, with additional coverage in CpG island shores and the regions flanking them. The samples were hybridized in the array following the manufacturer’s instructions.
 
Overall design Total DNA isolated by standard procedures from CD14+ cells (total monocytes, MOs) corresponding to three sets of samples of monocytes (MOs) and derived MACs and OCs following incubation with M-CSF or M-CSF/RANKL for 5 and 20 days.
 
Contributor(s) Garcia-Gomez A, Li T, Kerick M, Català-Moll F, Comet NR, Rodríguez-Ubreva J, de la Rica L, Branco MR, Martín J, Ballestar E
Citation(s) 28973458
Submission date Apr 07, 2017
Last update date Mar 22, 2019
Contact name Esteban Ballestar
Organization name Josep Carreras Research Institute (IJC)
Lab Epigenetics and Immune Disease
Street address Ctra de Can Ruti, Camí de les Escoles s/n
City Badalona, Barcelona
State/province N/A = Not Applicable
ZIP/Postal code 08916
Country Spain
 
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (30)
GSM2570249 BS-MO D1
GSM2570250 BS-MO D2
GSM2570251 BS-MO D3
Relations
BioProject PRJNA382126

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE97497_RAW.tar 183.1 Mb (http)(custom) TAR
GSE97497_normalized_avg_beta.txt.gz 86.1 Mb (ftp)(http) TXT
GSE97497_signal_intensities.txt.gz 69.9 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table
Processed data are available on Series record

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