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| Status |
Public on Jul 19, 2017 |
| Title |
Comparison of the DNA methylation profiles of CD14+ monocytes from human peripheral blood with derived macrophages (MACs) and osteoclasts (OCs) obtained by exposure with M-CSF and M-CSF/RANKL, respectively, for 5 and 20 days |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
The methylation profiles of bisulfite(BS)- and oxidative-BS-modified DNA of human CD14+ monocytes were compared with derived macrophages (MACs) and osteoclasts (OCs) following M-CSF and M-CSF/RANKL incubation for 5 and 20 days, using the Infinium HumanMethylation450 BeadChips (Illumina, Inc., San Diego, CA,). This platform allows the interrogation of >485,000 methylation sites per sample at single-nucleotide resolution, and comprises an average of 17 CpG sites per gene in the 99% of RefSeq genes. 96% of CpG islands are covered, with additional coverage in CpG island shores and the regions flanking them. The samples were hybridized in the array following the manufacturer’s instructions.
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| Overall design |
Total DNA isolated by standard procedures from CD14+ cells (total monocytes, MOs) corresponding to three sets of samples of monocytes (MOs) and derived MACs and OCs following incubation with M-CSF or M-CSF/RANKL for 5 and 20 days.
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| Contributor(s) |
Garcia-Gomez A, Li T, Kerick M, Català-Moll F, Comet NR, Rodríguez-Ubreva J, de la Rica L, Branco MR, Martín J, Ballestar E |
| Citation(s) |
28973458 |
| Submission date |
Apr 07, 2017 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Esteban Ballestar |
| Organization name |
Josep Carreras Research Institute (IJC)
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| Lab |
Epigenetics and Immune Disease
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| Street address |
Ctra de Can Ruti, Camí de les Escoles s/n
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| City |
Badalona, Barcelona |
| State/province |
N/A = Not Applicable |
| ZIP/Postal code |
08916 |
| Country |
Spain |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA382126 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE97497_RAW.tar |
183.1 Mb |
(http)(custom) |
TAR |
| GSE97497_normalized_avg_beta.txt.gz |
86.1 Mb |
(ftp)(http) |
TXT |
| GSE97497_signal_intensities.txt.gz |
69.9 Mb |
(ftp)(http) |
TXT |
| Raw data are available on Series record |
| Processed data included within Sample table |
| Processed data are available on Series record |
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