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Series GSE93117 Query DataSets for GSE93117
Status Public on Jan 05, 2017
Title Genetic dissection of nutrition-induced plasticity in insulin/insulin-like growth factor signaling in a Drosophila MPP
Organism Drosophila melanogaster
Experiment type Expression profiling by RT-PCR
Summary The nutritional environments that organisms experience are inherently variable, requiring tight coordination of how resources are allocated to different functions relative to the total amount of resources available. A growing body of evidence supports the hypothesis that key endocrine pathways play a fundamental role in this coordination. In particular, the insulin/insulin-like growth factor signaling (IIS) and target of rapamycin (TOR) pathways have been implicated in nutrition-dependent changes in metabolism and nutrient allocation, however little is known about the genetic basis of standing variation in the pathways. To characterize natural genetic variation in the IIS/TOR pathway, we used over 250 recombinant inbred lines (RILs) derived from a multiparental mapping population, the Drosophila Synthetic Population Resource (DSPR), to map QTL for transcript levels of the genes encoding 52 of the core components of the IIS/TOR pathway in three different nutritional environments (dietary restriction, control, and high sugar). In addition, we assayed lifespan for 80 RILs in both dietary restriction and control diets. We identified cis (i.e., local) QTL for six transcripts, all of which are significant in multiple nutrient environments. Further, we identified trans (i.e., distant) QTL for two transcripts, specific to a single nutrient environment, and one “plasticity” QTL that influences the change in gene expression between the control and high sugar diet. Nearly all transcripts, 85%, were significantly differentially expressed between diets. A discriminant function analysis for the control and dietary restriction treatments identified which transcripts best predict the diet treatment. The resulting composite discriminant function scores are correlated with median lifespan (r = 0.45) and mapping these scores revealed a significant QTL within the dietary restriction diet. Our results corroborate the association between the IIS/TOR pathway and the increase in lifespan under dietary restriction in a natural population and identify new locations in the genome involved in regulating the IIS/TOR pathway.
 
Overall design We have 918 total samples stemming from 259 recombinant inbred lines placed on three different diets. There is one replicate per line per diet for most samples with 20-30 flies pooled for each. The diets include dietary restriction, control, and high sugar. All fold change measurements are given relative to the control diet.
 
Contributor(s) Stanley PD, Ng'oma E, O'Day S, King EG
Citation(s) 28592498
NIH grant(s)
Grant ID Grant title Affiliation Name
F32 GM099382 The genetics of the Drosophila IIS pathway response to changing nutrition The Regents of the University of California Elizabeth Griep King
R01 GM117135 Coordinating nutrition and energy allocation: mechanisms and evolution UNIVERSITY OF MISSOURI-COLUMBIA Elizabeth Griep King
Submission date Jan 04, 2017
Last update date Jun 19, 2017
Contact name Elizabeth G King
E-mail(s) kingeg@missouri.edu
Organization name University of Missouri
Department Biological Sciences
Street address 105 Tucker Hall
City Columbia
State/province MO
ZIP/Postal code 65211-7400
Country USA
 
Platforms (1)
GPL22881 ThermoFisher Taqman® OpenArray® Drosophila melanogaster Custom 56 Assay Plate
Samples (918)
GSM2444068 22350.HS.1 (1.1)
GSM2444069 22138.DR.1 (10.1)
GSM2444070 22378.C.1 (100.1)
Relations
BioProject PRJNA360095

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE93117_fold-change.txt.gz 139.8 Kb (ftp)(http) TXT
GSE93117_non-normalized.txt.gz 198.6 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data is available on Series record
Processed data included within Sample table
Processed data is available on Series record

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