|
| Status |
Public on Jan 05, 2017 |
| Title |
22299.HS.1 (18.1) |
| Sample type |
RNA |
| |
|
| Source name |
whole fly
|
| Organism |
Drosophila melanogaster |
| Characteristics |
recombinant inbred line: 22299
treatment: high sugar
batch: batch 1
|
| Growth protocol |
Temperature: 23C; Humidity: 50%; Light cycle 24:0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction followed by a Qiagen clean up
|
| Label |
NA
|
| Label protocol |
Applied Biosystems High Capacity Reverse Transcription kit
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
18.1
|
| Data processing |
We used Ribosomal Protein L32 (RPL32) to normalize our expression measures using 2^-ΔCq. All fold changes were calculated relative to the control diet using 2^-ΔΔCq.
|
| |
|
| Submission date |
Jan 04, 2017 |
| Last update date |
Jan 05, 2017 |
| Contact name |
Elizabeth G King |
| E-mail(s) |
kingeg@missouri.edu
|
| Organization name |
University of Missouri
|
| Department |
Biological Sciences
|
| Street address |
105 Tucker Hall
|
| City |
Columbia |
| State/province |
MO |
| ZIP/Postal code |
65211-7400 |
| Country |
USA |
| |
|
| Platform ID |
GPL22881 |
| Series (1) |
| GSE93117 |
Genetic dissection of nutrition-induced plasticity in insulin/insulin-like growth factor signaling in a Drosophila MPP |
|
