|
Status |
Public on Jan 05, 2017 |
Title |
22420.HS.1 (196.1) |
Sample type |
RNA |
|
|
Source name |
whole fly
|
Organism |
Drosophila melanogaster |
Characteristics |
recombinant inbred line: 22420 treatment: high sugar batch: batch 1
|
Growth protocol |
Temperature: 23C; Humidity: 50%; Light cycle 24:0
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction followed by a Qiagen clean up
|
Label |
NA
|
Label protocol |
Applied Biosystems High Capacity Reverse Transcription kit
|
|
|
Hybridization protocol |
n/a
|
Scan protocol |
n/a
|
Description |
196.1
|
Data processing |
We used Ribosomal Protein L32 (RPL32) to normalize our expression measures using 2^-ΔCq. All fold changes were calculated relative to the control diet using 2^-ΔΔCq.
|
|
|
Submission date |
Jan 04, 2017 |
Last update date |
Jan 05, 2017 |
Contact name |
Elizabeth G King |
E-mail(s) |
kingeg@missouri.edu
|
Organization name |
University of Missouri
|
Department |
Biological Sciences
|
Street address |
105 Tucker Hall
|
City |
Columbia |
State/province |
MO |
ZIP/Postal code |
65211-7400 |
Country |
USA |
|
|
Platform ID |
GPL22881 |
Series (1) |
GSE93117 |
Genetic dissection of nutrition-induced plasticity in insulin/insulin-like growth factor signaling in a Drosophila MPP |
|