|
| Status |
Public on Jan 01, 2014 |
| Title |
Gene expression profile of wildtype glioblastoma cell lines and their resistant subclones |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In order to identify factors involved in TMZ-resistance, we engineered different TMZ-resistant glioblastoma cell lines. Wildtype cells were treated twice a week in duplicate with a clinically relevant concentration of TMZ (33 µM) for multiple weeks, until two individual resistant subclones were generated. Gene expression profiling was performed to identify differentially expressed genes in the resistant cells compared to their wildtype cells.
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| Overall design |
three wildtype glioblastoma cell lines (U87, LNZ308, Hs683) and their resistant subclones, two of each wildtype, were analyzed
The experiments were performed technically as dual channel but processed/normalized as single channel (i.e. two sample records per one raw data file). The raw data file for each sample is indicated in the sample description field but linked as Series supplementary file.
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| |
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| Contributor(s) |
Hiddingh L, Wurdinger T |
| Citation(s) |
24495907 |
| |
| Submission date |
Dec 05, 2013 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Lotte Hiddingh |
| E-mail(s) |
l.hiddingh@vumc.nl
|
| Organization name |
VU University Medical Center
|
| Lab |
Neuro-Oncology Research Group
|
| Street address |
De Boelelaan 1117
|
| City |
Amsterdam |
| ZIP/Postal code |
1081 HV |
| Country |
Netherlands |
| |
|
| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA230678 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE53014_RAW.tar |
6.9 Mb |
(http)(custom) |
TAR |
| GSE53014_US22502676_251485064817_S01_GE2-v5_95_Feb07_1_1.txt.gz |
15.4 Mb |
(ftp)(http) |
TXT |
| GSE53014_US22502676_251485064817_S01_GE2-v5_95_Feb07_1_2.txt.gz |
15.4 Mb |
(ftp)(http) |
TXT |
| GSE53014_US22502676_251485064817_S01_GE2-v5_95_Feb07_1_3.txt.gz |
15.4 Mb |
(ftp)(http) |
TXT |
| GSE53014_US22502676_251485064817_S01_GE2-v5_95_Feb07_1_4.txt.gz |
15.3 Mb |
(ftp)(http) |
TXT |
| GSE53014_US22502676_251485064818_S01_GE2-v5_95_Feb07_1_1.txt.gz |
15.4 Mb |
(ftp)(http) |
TXT |
| GSE53014_US22502676_251485064818_S01_GE2-v5_95_Feb07_1_2.txt.gz |
15.4 Mb |
(ftp)(http) |
TXT |
| GSE53014_US22502676_251485064818_S01_GE2-v5_95_Feb07_1_3.txt.gz |
15.4 Mb |
(ftp)(http) |
TXT |
| GSE53014_US22502676_251485064818_S01_GE2-v5_95_Feb07_1_4.txt.gz |
15.3 Mb |
(ftp)(http) |
TXT |
| GSE53014_US22502676_251485064820_S01_GE2-v5_95_Feb07_1_1.txt.gz |
15.3 Mb |
(ftp)(http) |
TXT |
| GSE53014_US22502676_251485064820_S01_GE2-v5_95_Feb07_1_2.txt.gz |
15.3 Mb |
(ftp)(http) |
TXT |
| GSE53014_US22502676_251485064820_S01_GE2-v5_95_Feb07_1_3.txt.gz |
15.3 Mb |
(ftp)(http) |
TXT |
| GSE53014_US22502676_251485064820_S01_GE2-v5_95_Feb07_1_4.txt.gz |
15.3 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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