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Series GSE129488 Query DataSets for GSE129488
Status Public on Jun 01, 2019
Title Transcriptional response of human synovial fibroblasts to TNF and IL-17A
Organism Homo sapiens
Experiment type Expression profiling by array
Expression profiling by high throughput sequencing
Summary This SuperSeries is composed of the SubSeries listed below.
 
Overall design Refer to individual Series:

Microarray Data 1 (n=60)
9 time points, 2 technical replicates, 5 stimulations (Untreated; Media; IL-17A (1 ng/mL); TNF (1 ng/mL); TNF and IL-17A)

Microarray Data 2 (n=48)
1 time point (24 hours), 3 passages, 4 cell lines, 4 stimulations (Baseline; None; TNF; TNF and IL-17A)

RNA-seq Data 1 (n=175)
7 synovial fibroblast cell lines from patient tissues, 9 time points (0, 2, 4, 6, 8, 10, 12, 18, and 24 hours), 3 stimulations (TNF (1 ng/mL); TNF (1 ng/mL) and IL-17A (1 ng/mL); TNF (1 ng/mL) and IL-17A (10 ng/mL))

RNA-seq Data 2 (n=192)
4 synovial fibroblast cell lines from patient tissues, 4 time points (0, 1, 6 and 16 hours), 2 stimulations (TNF (0.1 ng/mL); TNF (0.1 ng/mL) and IL-17A (1 ng/mL)), and 5 siRNAs (CUX1, LIFR, STAT3, STAT4, ELF3)
Web link https://doi.org/10.1101/571315
 
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NIH grant(s)
Grant ID Grant title Affiliation Name
F31 AR070582 Transcriptomics in synoviocytes defines pathogenesis of rheumatoid arthritis HARVARD UNIVERSITY (MEDICAL SCHOOL) Kamil Slowikowski
Submission date Apr 08, 2019
Last update date Jul 12, 2019
Contact name Kamil Slowikowski
Organization name Harvard University
Lab Soumya Raychaudhuri
Street address 77 Avenue Louis Pasteur, Suite 255
City Boston
State/province Massachusetts
ZIP/Postal code 02115
Country USA
 
Platforms (3)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (475)
GSM3713851 EA10047_314601_HUGENE-1_0-ST-V1_HU.FB.0HR.UNTREATED#1
GSM3713852 EA10047_314602_HUGENE-1_0-ST-V1_HU.FB.0HR.UNTREATED#2
GSM3713853 EA10047_314603_HUGENE-1_0-ST-V1_HU.FB.1HR.MEDIA#1
This SuperSeries is composed of the following SubSeries:
GSE93720 Transcriptional response of human synovial fibroblasts to TNF and IL-17A (Microarray Data 2)
GSE129484 Transcriptional response of human synovial fibroblasts to TNF and IL-17A over time (Microarray Data 1)
GSE129486 Transcriptional responses of human synovial fibroblasts to TNF and IL-17A over time (RNA-seq Data 1)
Relations
BioProject PRJNA531476

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE129488_RAW.tar 480.6 Mb (http)(custom) TAR (of CEL)
SRA Run SelectorHelp

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