|
Status |
Public on Jun 01, 2019 |
Title |
S112 time-course |
Sample type |
SRA |
|
|
Source name |
synovium
|
Organism |
Homo sapiens |
Characteristics |
plate: 2 well: G06 disease: OA donor: OA2 stimulation: TNF (1) + IL17 (1) time: 10
|
Treatment protocol |
Cells were stimulated with TNF (1 ng/mL) or TNF (1 ng/mL) + IL-17A (1 ng/mL) on day 3 for 0, 2, 4 ,6, 8, 10, 12, 18, 24 hours.
|
Growth protocol |
Fibroblasts were plated on day 1 at 50,000 cells per well in 24-well plates in 10% FBS containing media. Cells were serum-starved on day 2 by changing to 1% FBS-containing media.
|
Extracted molecule |
polyA RNA |
Extraction protocol |
RNA was extracted using the Qiagen RNeasy Micro Kit. SMART-seq2
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Data processing |
kallisto version 0.43.1 Genome_build: Ensembl release 89 Supplementary_files_format_and_content: tab-separated files, estimated counts, transcripts per million (TPM)
|
|
|
Submission date |
Apr 08, 2019 |
Last update date |
Jun 01, 2019 |
Contact name |
Kamil Slowikowski |
E-mail(s) |
kslowikowski@mgh.harvard.edu
|
Organization name |
Massachusetts General Brigham
|
Lab |
Alexandra-Chloe Villani
|
Street address |
Mailstop CNY 149-8, 149 13th Street
|
City |
Charlestown |
State/province |
Massachusetts |
ZIP/Postal code |
02129 |
Country |
USA |
|
|
Platform ID |
GPL11154 |
Series (2) |
GSE129486 |
Transcriptional responses of human synovial fibroblasts to TNF and IL-17A over time (RNA-seq Data 1) |
GSE129488 |
Transcriptional response of human synovial fibroblasts to TNF and IL-17A |
|
Relations |
BioSample |
SAMN11359667 |