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| Status |
Public on Aug 15, 2008 |
| Title |
Suppression of oleosin in soybean cotyledon |
| Organism |
Glycine max |
| Experiment type |
Expression profiling by array
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| Summary |
3 samples, 2 reps each. comparison of wildtype cotyledon to RNAioleosin transgenic
Using RNAi, the seed oil body protein 24-kDa oleosin has been suppressed in transgenic soybeans. The endoplasmic reticulum (ER) forms micro-oil bodies about 50 nm in diameter that coalesce with adjacent oil bodies forming a hierarchy of oil body sizes. The oil bodies in the oleosin knockdown form large oil body-ER complexes with the interior dominated by micro-oil bodies and intermediate-sized oil bodies, while the peripheral areas of the complex are dominated by large oil bodies. The complex merges to form giant oil bodies with onset of seed dormancy that disrupts cell structure. The transcriptome of the oleosin knockdown shows few changes compared to wild-type. Proteomic analysis of the isolated oil bodies of the 24-kDa oleosin knockdown shows the absence of the 24-kDa oleosin and the presence of abundant caleosin and lipoxygenase. The formation of the micro-oil bodies in the oleosin knockdown is interpreted to indicate a function of the oleosin as a surfactant.
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| Overall design |
2 biological replicates of transgenics, 2 technical replicates each
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| Contributor(s) |
Schmidt MA, Herman EM |
| Citation(s) |
19825592 |
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| Submission date |
Jul 30, 2008 |
| Last update date |
Nov 22, 2016 |
| Contact name |
monica schmidt |
| Organization name |
danforth center
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| Lab |
herman
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| Street address |
975 n. warson
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| City |
st.louis |
| State/province |
MO |
| ZIP/Postal code |
63132 |
| Country |
USA |
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| Platforms (1) |
| GPL4592 |
[Soybean] Affymetrix Soybean Genome Array |
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| Samples (6)
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| GSM309344 |
transgenic RNAiOleosin, biol rep 1, tech rep 2 |
| GSM309345 |
transgenic RNAiOleosin, biol rep 2, tech rep 1 |
| GSM309346 |
transgenic RNAiOleosin, biol rep 2, tech rep 2 |
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| Relations |
| BioProject |
PRJNA113599 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE12300_RAW.tar |
29.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
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