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Series GSE12300 Query DataSets for GSE12300
Status Public on Aug 15, 2008
Title Suppression of oleosin in soybean cotyledon
Organism Glycine max
Experiment type Expression profiling by array
Summary 3 samples, 2 reps each. comparison of wildtype cotyledon to RNAioleosin transgenic
Using RNAi, the seed oil body protein 24-kDa oleosin has been suppressed in transgenic soybeans. The endoplasmic reticulum (ER) forms micro-oil bodies about 50 nm in diameter that coalesce with adjacent oil bodies forming a hierarchy of oil body sizes. The oil bodies in the oleosin knockdown form large oil body-ER complexes with the interior dominated by micro-oil bodies and intermediate-sized oil bodies, while the peripheral areas of the complex are dominated by large oil bodies. The complex merges to form giant oil bodies with onset of seed dormancy that disrupts cell structure. The transcriptome of the oleosin knockdown shows few changes compared to wild-type. Proteomic analysis of the isolated oil bodies of the 24-kDa oleosin knockdown shows the absence of the 24-kDa oleosin and the presence of abundant caleosin and lipoxygenase. The formation of the micro-oil bodies in the oleosin knockdown is interpreted to indicate a function of the oleosin as a surfactant.
Overall design 2 biological replicates of transgenics, 2 technical replicates each
Contributor(s) Schmidt MA, Herman EM
Citation(s) 19825592
Submission date Jul 30, 2008
Last update date Nov 22, 2016
Contact name monica schmidt
Organization name danforth center
Lab herman
Street address 975 n. warson
City st.louis
State/province MO
ZIP/Postal code 63132
Country USA
Platforms (1)
GPL4592 [Soybean] Affymetrix Soybean Genome Array
Samples (6)
GSM309341 Wild type 1
GSM309342 Wild type 2
GSM309343 transgenic RNAiOleosin, biol rep 1, tech rep 1
BioProject PRJNA113599

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE12300_RAW.tar 29.7 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table

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