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    EEF1A1 eukaryotic translation elongation factor 1 alpha 1 [ Homo sapiens (human) ]

    Gene ID: 1915, updated on 5-May-2024

    Summary

    Official Symbol
    EEF1A1provided by HGNC
    Official Full Name
    eukaryotic translation elongation factor 1 alpha 1provided by HGNC
    Primary source
    HGNC:HGNC:3189
    See related
    Ensembl:ENSG00000156508 MIM:130590; AllianceGenome:HGNC:3189
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; EF1A1; LENG7; eEF1A-1; GRAF-1EF; EF1alpha1
    Summary
    This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008]
    Expression
    Ubiquitous expression in ovary (RPKM 2994.9), thyroid (RPKM 2551.7) and 25 other tissues See more
    Orthologs
    NEW
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    Try the new Transcript table

    Genomic context

    See EEF1A1 in Genome Data Viewer
    Location:
    6q13
    Exon count:
    8
    Annotation release Status Assembly Chr Location
    RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 6 NC_000006.12 (73515750..73521032, complement)
    RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 6 NC_060930.1 (74692854..74698136, complement)
    105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 6 NC_000006.11 (74225473..74230755, complement)

    Chromosome 6 - NC_000006.12Genomic Context describing neighboring genes Neighboring gene RNA, U6 small nuclear 975, pseudogene Neighboring gene ATAC-STARR-seq lymphoblastoid active region 24747 Neighboring gene mitochondrial tRNA translation optimization 1 Neighboring gene RNA, 7SL, cytoplasmic 827, pseudogene Neighboring gene MPRA-validated peak5897 silencer Neighboring gene Sharpr-MPRA regulatory region 3585 Neighboring gene H3K27ac hESC enhancer GRCh37_chr6:74229093-74229915 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr6:74229916-74230737 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr6:74230738-74231560 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 17336 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 17337 Neighboring gene EEF1A1 antisense RNA 1 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 24751 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 24752 Neighboring gene H3K27ac hESC enhancer GRCh37_chr6:74273247-74273796 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr6:74275447-74275996 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr6:74285738-74286453 Neighboring gene P300/CBP strongly-dependent group 1 enhancer GRCh37_chr6:74289636-74290835 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr6:74291165-74292007 Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_94401 Neighboring gene solute carrier family 17 member 5 Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_94402 Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_94403 Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_94405 Neighboring gene ribosomal protein S27 pseudogene 15

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    HIV-1 interactions

    Replication interactions

    Interaction Pubs
    HIV-1 NL4-3 replication is restricted by EEF1A1 as enhanced replication was discovered when EEF1A1 is deleted through CRISPR/Cas9 genome editing in primary CD4+ T cells PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Envelope surface glycoprotein gp120 env Tandem affinity purification and mass spectrometry analysis identify eukaryotic translation elongation factor 1 alpha 1 (EEF1A1), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    Gag-Pol gag-pol Tandem affinity purification and mass spectrometry analysis identify eukaryotic translation elongation factor 1 alpha 1 (EEF1A1), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    Nef nef eEF1A/Nef complexes contain tRNAs and block stress-induced apoptosis in monocyte-derived macrophages through tRNA binding to cytochrome c PubMed
    nef Exp-t-mediated nuclear-cytoplasmic transport of the eEF1A/Nef complexes in monocyte-derived macrophages is invloved in the inhibition of apoptosis triggered by stress PubMed
    nef HIV-1 Nef binds eEF1A in cells. Amino acids 55 to 206 in Nef and amino acids 1-74 in eEF1A are sufficient for the binding PubMed
    nef Yeast two hybrid assay identifies the HIV-1 Nef-interacting protein translational elongation factor 1 PubMed
    nef Tandem affinity purification and mass spectrometry analysis identify eukaryotic translation elongation factor 1 alpha 1 (EEF1A1), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    Pr55(Gag) gag Cellular biotinylated eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) protein is incorporated into HIV-1 Gag virus-like particles PubMed
    gag Tandem affinity purification and mass spectrometry analysis identify eukaryotic translation elongation factor 1 alpha 1 (EEF1A1), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    gag The interaction between the Matrix protein of HIV-1 Gag and elongation factor 1-alpha impairs translation in vitro, suggesting a role for this interaction in releasing viral RNA from polysomes, permitting the RNA to be packaged into virions PubMed
    gag The basic region of the Matrix protein of HIV-1 Gag (amino acids 15-35) mediates binding to amino acids 1-74 of elongation factor 1-alpha PubMed
    gag Binding of elongation factor 1-alpha to HIV-1 Gag proteins (Matrix and Nucleocapsid) appears to be mediated by RNA PubMed
    gag Elongation factor 1-alpha binds to HIV-1 Matrix, as well as to HIV-1 Gag and Nucleocapsid and is incorporated into HIV-1 virion membranes PubMed
    Rev rev HIV-1 Rev interacting protein, eukaryotic translation elongation factor 1 alpha 1 (EEF1A1), is identified by the in-vitro binding experiments involving cytosolic or nuclear extracts from HeLa cells PubMed
    Tat tat Eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) is identified to interact with HIV-1 Tat mutant Nullbasic in HeLa cells by LC MS/MS PubMed
    tat EF-1alpha stimulates the binding of RNA Polymerase II and TRP-185 to HIV-1 TAR RNA, suggesting an interaction between EF-1alpha and HIV-1 Tat PubMed
    integrase gag-pol HIV-1 IN co-immunoprecipitates with eEF1A through an interaction with the viral RTCs PubMed
    gag-pol Using acetylated HIV-1 IN as bait in yeast two-hybrid screening identifies translation regulatory and RNA binding proteins eIF3h, eEF1A-1, and hnRNPA2 as IN-binding partners PubMed
    gag-pol Interaction of human EF1alpha with HIV-1 integrase is presumed based on a reported binding interaction between yeast TEF1alpha and integrase PubMed
    matrix gag The interaction between HIV-1 Matrix and elongation factor 1-alpha impairs translation in vitro, suggesting a role for this interaction in releasing viral RNA from polysomes, permitting the RNA to be packaged into virions PubMed
    gag The basic region of HIV-1 Matrix (amino acids 15-35) mediates binding to amino acids 1-74 of elongation factor 1-alpha PubMed
    gag Binding of elongation factor 1-alpha to HIV-1 Gag proteins (Matrix and Nucleocapsid) appears to be mediated by RNA PubMed
    gag Elongation factor 1-alpha binds to HIV-1 Matrix, as well as to HIV-1 Gag and Nucleocapsid and is incorporated into HIV-1 virion membranes PubMed
    reverse transcriptase p51 subunit gag-pol HIV-1 RT interacts with EEF1A1 as shown through biolayer interferometry and co-immunoprecipitation and can be abrogated by mutating amino acid W252 in RT PubMed
    gag-pol HIV-1 RT p51 subunit co-immunoprecipitates with eEF1A through an interaction with the viral RTCs PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Clone Names

    • FLJ25721, MGC16224, MGC102687, MGC131894

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables GTP binding TAS
    Traceable Author Statement
    more info
    PubMed 
    enables GTPase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables GTPase activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables RNA binding HDA PubMed 
    enables kinase binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables molecular adaptor activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables protein kinase binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables tRNA binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables translation elongation factor activity IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables translation elongation factor activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables translation elongation factor activity TAS
    Traceable Author Statement
    more info
    PubMed 
    Component Evidence Code Pubs
    located_in cortical actin cytoskeleton IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in cytoplasm ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    located_in cytoplasm TAS
    Traceable Author Statement
    more info
    PubMed 
    located_in cytoplasmic side of lysosomal membrane NAS
    Non-traceable Author Statement
    more info
    PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in cytosol TAS
    Traceable Author Statement
    more info
     
    is_active_in cytosolic ribosome IDA
    Inferred from Direct Assay
    more info
    PubMed 
    part_of eukaryotic translation elongation factor 1 complex TAS
    Traceable Author Statement
    more info
    PubMed 
    located_in extracellular exosome HDA PubMed 
    located_in extracellular region TAS
    Traceable Author Statement
    more info
     
    located_in extracellular space HDA PubMed 
    located_in ficolin-1-rich granule lumen TAS
    Traceable Author Statement
    more info
     
    located_in membrane HDA PubMed 
    located_in nucleolus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in nucleus HDA PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    PubMed 
    is_active_in ribosome IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in ruffle membrane IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in secretory granule lumen TAS
    Traceable Author Statement
    more info
     

    General protein information

    Preferred Names
    elongation factor 1-alpha 1
    Names
    CTCL tumor antigen
    EF-1-alpha-1
    EF1a-like protein
    cervical cancer suppressor 3
    elongation factor 1 alpha subunit
    elongation factor Tu
    epididymis secretory sperm binding protein
    eukaryotic elongation factor 1 A-1
    eukaryotic translation elongation factor 1 alpha 1-like 14
    glucocorticoid receptor AF-1 specific elongation factor
    leukocyte receptor cluster (LRC) member 7
    leukocyte receptor cluster member 7
    prostate tumor-inducing protein 1
    translation elongation factor 1 alpha 1-like 14

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001402.6NP_001393.1  elongation factor 1-alpha 1

      See identical proteins and their annotated locations for NP_001393.1

      Status: REVIEWED

      Source sequence(s)
      AJ420488, AL603910, BC066893
      Consensus CDS
      CCDS4980.1
      UniProtKB/Swiss-Prot
      P04719, P04720, P68104, Q6IQ15
      UniProtKB/TrEMBL
      A8K9C4, Q53G85, Q53GA1, Q53GE9, Q53HM9, Q53HQ7, Q53HR5, Q6IPN6, Q6IPS9, Q6IPT9
      Related
      ENSP00000339053.4, ENST00000309268.11
      Conserved Domains (1) summary
      PTZ00141
      Location:1460
      PTZ00141; elongation factor 1- alpha; Provisional

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000006.12 Reference GRCh38.p14 Primary Assembly

      Range
      73515750..73521032 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060930.1 Alternate T2T-CHM13v2.0

      Range
      74692854..74698136 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054354427.1XP_054210402.1  elongation factor 1-alpha 1 isoform X1

      UniProtKB/Swiss-Prot
      P04719, P04720, P68104, Q6IQ15
      UniProtKB/TrEMBL
      Q6IPS9

    Suppressed Reference Sequence(s)

    The following Reference Sequences have been suppressed. Explain

    1. NM_001403.1: Suppressed sequence

      Description
      NM_001403.1: This RefSeq was permanently suppressed because it was based on a chimeric sequence and represented a partial CDS.