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CUL5 cullin 5 [ Homo sapiens (human) ]

Gene ID: 8065, updated on 9-Apr-2023


Official Symbol
CUL5provided by HGNC
Official Full Name
cullin 5provided by HGNC
Primary source
See related
Ensembl:ENSG00000166266 MIM:601741; AllianceGenome:HGNC:2556
Gene type
protein coding
RefSeq status
Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
Enables ubiquitin protein ligase binding activity. Predicted to be involved in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process and protein ubiquitination. Predicted to act upstream of or within cerebral cortex radially oriented cell migration and radial glia guided migration of Purkinje cell. Located in site of DNA damage. Part of Cul5-RING ubiquitin ligase complex. [provided by Alliance of Genome Resources, Apr 2022]
Ubiquitous expression in thyroid (RPKM 9.9), kidney (RPKM 8.6) and 25 other tissues See more
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Genomic context

See CUL5 in Genome Data Viewer
Exon count:
Annotation release Status Assembly Chr Location
RS_2023_03 current GRCh38.p14 (GCF_000001405.40) 11 NC_000011.10 (108008898..108107761)
RS_2023_03 current T2T-CHM13v2.0 (GCF_009914755.1) 11 NC_060935.1 (108015935..108114778)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 11 NC_000011.9 (107879624..107978488)

Chromosome 11 - NC_000011.10Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC124902747 Neighboring gene RAB39A, member RAS oncogene family Neighboring gene Sharpr-MPRA regulatory region 4319 Neighboring gene uncharacterized LOC124902748 Neighboring gene acetyl-CoA acetyltransferase 1

Genomic regions, transcripts, and products


  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018


GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Tat tat Cullin 5 is downregulated in HIV-1 Tat and NC cotransfection of HEK 293T cells PubMed
Vif vif HIV-1 Vif interacts with CUL5 PubMed
vif Both Cul5-Rbx1 and Cul5-Rbx2 interactions promote APOBEC3G polyubiquitination in vitro and this promotion depends on the presence of the Vif-CBFbeta-EloB-EloC complex PubMed
vif HIV-1 Vif ubiquitination is promoted by Cul5 in vitro and in vivo in a manner that requires an intact SOCS-box motif in Vif, and auto ubiquitination of Vif occurs within an assembled Vif-Cul5 complex PubMed
vif CUL5/RBX2/ELOB/ELOC/Vif/CBF-beta complex catalyzes polyubiquitin chain formation on A3G in the presence of ubiquitin E2 UBE2R1 (CDC34) or UBCH5b (UBE2D2) PubMed
vif HIV-1 Vif, CBF-beta, CUL5, and ELOB/C form a complex that is required for Vif-mediated downregulation of A3G and A3F. CBF-beta regulates HIV-1 infectivity only in the presence of A3G PubMed
vif Two highly conserved Cys residues (C114 and C133) outside the SOCS box (amino acids 144-169) motif in HIV-1 Vif are required for the interaction of Vif with Cul5 but not ElonginC PubMed
vif HIV-1 Vif (amino acids 144-149; SLQXLA motif; BC-box) interacts with cellular proteins Cul5, elongins B and C, and Rbx1 to form an Skp1-Cullin-F-box (SCF)-like complex that allows Vif to interact with APOBEC3G and induce its ubiquitination and degradation PubMed
vif Amino-acid motifs 84GxSIEW89 and 102LADQLI107 in HIV-1 Vif affect its interaction with CUL5, and Vif mutants 84DVAAAA89, 88EW/AA89, G84A, G84D, W89A, S104A, L106S, and I107S have more than 50% reduction in CUL5 binding PubMed
vif UBE2F and RBX2 are required for activation of the polyubiquitin synthesis activity of Vif/CBF-beta/CUL5, leading to HIV-1 Vif-mediated degradation of A3G in cells PubMed
vif The T(Q/D/E)x(5)ADx(2)(I/L) motif, located at residues 96 to 107 in HIV-1 Vif, regulates Vif interaction with Cul5 PubMed
vif Simultaneous substitution of the three Vif-interacting residues L52, W53, and D55 and the two ELOC-interacting residues P41 and H48 in CUL5 impairs the ability of CUL5 to interact with the Vif-CBF-beta-ELOB-ELOC protein complex PubMed
vif The absence of Vif-CBF-beta reduces the interaction between the CUL5 and the EloC-EloB complex, indicating that the former two proteins have a critical role in promoting assembly of the pentameric complex PubMed
vif An overall crystal structure indicates that the Vif-CBF-beta-CUL5-ELOB-ELOC complex has a U-shape architecture, including the two straight arms Vif-CBF-beta and CUL5 and the bent arm formation between ELOC and CUL5 and Vif interactions PubMed
vif The interaction between Cul5 and HIV-1, HIV-2, SIVmac, or SIVagm Vif protein require the presence of zinc PubMed
vif The HIV-1 Vif N-terminal motif (residues 18-38) binds CUL5 in mammalian cells and is reguired for A3F and A3G degradation and HIV-1 infectivity PubMed
vif HIV-1 Vif mutants C114S and C133A abolish the interaction with CUL5, which leads to fail to A3G degradation by Vif PubMed
vif HIV-1 Vif YF111/112AA, F115A, I120S and AL123/124SS mutants are unable to recruit Cul5, but retain interactions with Elongin B and C proteins PubMed
vif Deletion of amino acids 120 to 138 in Cul5 significantly reduces its interaction with HIV-1 Vif, but does not affect Cul5 binding to Elongins B/C; the HCCH zinc-binding motif (residues 108-139) in Vif is required for the interaction with Cul5 PubMed
vif The interaction between the HIV-1 Vif PPLP motif (residues 161-164) and the 34-amino-acid C-terminal tail (residues 85-118) of EloB plays a role in promoting recruitment of CBF-beta to the Vif-Cul5 E3 complex PubMed
vif The conjugation of NEDD8 to Cullin-5 by UBE2F is required for HIV-1 Vif-mediated A3G degradation PubMed
vif CBF-beta and the N-terminal half of HIV-1 Vif enhance the affinity of Cul5 for Vif PubMed
vif HIV-1 Vif-induced G2 accumulation requires a Cul5-based E3 ligase, but is independent of APOBEC3D/E, F, and G expression. Overexpression of ubiquitin(K48R) abolishes Vif-induced G2 accumulation PubMed
vif Mutations in HIV-1 Vif PPLP motif (amino acids 161-164) reduces Vif binding to A3G without affecting its interaction with ElonginC and Cullin5 PubMed
vif Chim3, corresponding to amino acids 126-170 of the natural mutant F12-Vif, interacts poorly with Cul5 but affects HIV-1 Vif/Cul5 interaction PubMed
vif Vif-induced ubiquitination of A3G and A3G20K/R is inhibited by Cul5deltaNedd8 PubMed
vif HIV-1 Vif is regulated by Cullin5 E3 ligase and its expression levels increases in the presence of a Cullin5 dominant negative mutant, Cul5deltaNedd8 PubMed
vif The amino acids L163 and L169 located downstream of the SOCS-box motif in HIV-1 Vif are required for Vif function and efficient interaction with Cul5-ElonginB-ElonginC PubMed
vif Treatment with the membrane-permeable zinc chelator TPEN prevents Vif function, and causes the blockage of Cul5 recruitment and APOBEC3G (A3G) degradation PubMed
nucleocapsid gag Cullin 5 is downregulated in HIV-1 Tat and NC cotransfection of HEK 293T cells PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem


Products Interactant Other Gene Complex Source Pubs Description

General gene information


Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables calcium channel activity TAS
Traceable Author Statement
more info
enables protein binding IPI
Inferred from Physical Interaction
more info
enables protein-macromolecule adaptor activity IBA
Inferred from Biological aspect of Ancestor
more info
enables signaling receptor activity TAS
Traceable Author Statement
more info
enables ubiquitin protein ligase binding IBA
Inferred from Biological aspect of Ancestor
more info
enables ubiquitin protein ligase binding IDA
Inferred from Direct Assay
more info
enables ubiquitin-protein transferase activity TAS
Traceable Author Statement
more info
Component Evidence Code Pubs
part_of Cul5-RING ubiquitin ligase complex IBA
Inferred from Biological aspect of Ancestor
more info
part_of Cul5-RING ubiquitin ligase complex IDA
Inferred from Direct Assay
more info
part_of SCF ubiquitin ligase complex IBA
Inferred from Biological aspect of Ancestor
more info
located_in cytosol TAS
Traceable Author Statement
more info
located_in nucleus IEA
Inferred from Electronic Annotation
more info
located_in site of DNA damage IDA
Inferred from Direct Assay
more info

General protein information

Preferred Names
Cullin-5 (vasopressin-activated calcium-mobilizing receptor-1)
Vasopressin-activated calcium-mobilizing receptor-1
vasopressin-activated calcium-mobilizing receptor 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_003478.6NP_003469.2  cullin-5

    See identical proteins and their annotated locations for NP_003469.2

    Status: VALIDATED

    Source sequence(s)
    AP002433, AP003307
    Consensus CDS
    Q93034, Q9BZC6
    ENSP00000376808.2, ENST00000393094.7
    Conserved Domains (2) summary
    Cullin; Cullin family
    Cullin_Nedd8; Cullin protein neddylation domain

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_03

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly


  1. NC_000011.10 Reference GRCh38.p14 Primary Assembly

    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017018363.3XP_016873852.1  cullin-5 isoform X1

  2. XM_005271682.3XP_005271739.1  cullin-5 isoform X2

    Conserved Domains (2) summary
    Cullin; Cullin family
    Cullin_Nedd8; Cullin protein neddylation domain
  3. XM_047427640.1XP_047283596.1  cullin-5 isoform X3

  4. XM_011543013.3XP_011541315.1  cullin-5 isoform X5

    Conserved Domains (1) summary
    Cullin; Cullin family
  5. XM_047427641.1XP_047283597.1  cullin-5 isoform X4

Alternate T2T-CHM13v2.0


  1. NC_060935.1 Alternate T2T-CHM13v2.0

    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054370045.1XP_054226020.1  cullin-5 isoform X1

  2. XM_054370046.1XP_054226021.1  cullin-5 isoform X2

  3. XM_054370047.1XP_054226022.1  cullin-5 isoform X3

  4. XM_054370049.1XP_054226024.1  cullin-5 isoform X5

  5. XM_054370048.1XP_054226023.1  cullin-5 isoform X4