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Ago4 argonaute RISC catalytic subunit 4 [ Mus musculus (house mouse) ]

Gene ID: 76850, updated on 8-Feb-2024

Summary

Official Symbol
Ago4provided by MGI
Official Full Name
argonaute RISC catalytic subunit 4provided by MGI
Primary source
MGI:MGI:1924100
See related
Ensembl:ENSMUSG00000042500 AllianceGenome:MGI:1924100
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Eif2c4; 5730550L01Rik
Summary
Enables RNA binding activity. Acts upstream of or within several processes, including male gonad development; meiotic nuclear division; and miRNA metabolic process. Located in P-body and nucleus. Part of RISC complex. Is expressed in several structures, including brain; lung; neural tube; tooth; and vibrissa. Orthologous to human AGO4 (argonaute RISC component 4). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Broad expression in whole brain E14.5 (RPKM 3.1), CNS E18 (RPKM 2.7) and 26 other tissues See more
Orthologs
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Genomic context

See Ago4 in Genome Data Viewer
Location:
4 D2.2; 4 60.9 cM
Exon count:
19
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 4 NC_000070.7 (126383280..126427379, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 4 NC_000070.6 (126489487..126533586, complement)

Chromosome 4 - NC_000070.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_11400 Neighboring gene argonaute RISC catalytic subunit 3 Neighboring gene STARR-seq mESC enhancer starr_11401 Neighboring gene STARR-positive B cell enhancer ABC_E7988 Neighboring gene argonaute RISC catalytic subunit 1 Neighboring gene STARR-seq mESC enhancer starr_11403 Neighboring gene STARR-positive B cell enhancer ABC_E2714 Neighboring gene STARR-seq mESC enhancer starr_11404 Neighboring gene STARR-positive B cell enhancer ABC_E1655 Neighboring gene STARR-positive B cell enhancer mm9_chr4:126231790-126232090 Neighboring gene STARR-positive B cell enhancer ABC_E10259 Neighboring gene microRNA 7119 Neighboring gene claspin Neighboring gene 60S ribosomal protein L36a-like

Genomic regions, transcripts, and products

Expression

  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Variation

Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (1)  1 citation
  • Endonuclease-mediated (2) 

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Clone Names

  • KIAA1567

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables RNA binding IDA
Inferred from Direct Assay
more info
PubMed 
enables RNA endonuclease activity IBA
Inferred from Biological aspect of Ancestor
more info
 
enables double-stranded RNA binding ISO
Inferred from Sequence Orthology
more info
 
enables miRNA binding IBA
Inferred from Biological aspect of Ancestor
more info
 
enables miRNA binding ISO
Inferred from Sequence Orthology
more info
 
enables nucleic acid binding IEA
Inferred from Electronic Annotation
more info
 
enables single-stranded RNA binding IBA
Inferred from Biological aspect of Ancestor
more info
 
enables single-stranded RNA binding ISO
Inferred from Sequence Orthology
more info
 
Process Evidence Code Pubs
involved_in RISC complex assembly ISO
Inferred from Sequence Orthology
more info
 
involved_in RNA secondary structure unwinding ISO
Inferred from Sequence Orthology
more info
 
involved_in mRNA catabolic process ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of_or_within male gonad development IMP
Inferred from Mutant Phenotype
more info
PubMed 
acts_upstream_of_or_within male meiotic nuclear division IMP
Inferred from Mutant Phenotype
more info
PubMed 
acts_upstream_of_or_within miRNA metabolic process IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in miRNA processing ISO
Inferred from Sequence Orthology
more info
 
involved_in miRNA-mediated gene silencing by inhibition of translation ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of_or_within negative regulation of apoptotic process IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in pre-miRNA processing IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in pre-miRNA processing ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of_or_within regulation of cell morphogenesis IMP
Inferred from Mutant Phenotype
more info
PubMed 
acts_upstream_of_or_within regulation of translation IEA
Inferred from Electronic Annotation
more info
 
acts_upstream_of_or_within regulatory ncRNA-mediated gene silencing IEA
Inferred from Electronic Annotation
more info
 
involved_in regulatory ncRNA-mediated post-transcriptional gene silencing IBA
Inferred from Biological aspect of Ancestor
more info
 
acts_upstream_of_or_within synaptonemal complex assembly IMP
Inferred from Mutant Phenotype
more info
PubMed 
Component Evidence Code Pubs
located_in P-body IDA
Inferred from Direct Assay
more info
PubMed 
part_of RISC complex IBA
Inferred from Biological aspect of Ancestor
more info
 
part_of RISC complex IDA
Inferred from Direct Assay
more info
PubMed 
part_of RISC complex ISO
Inferred from Sequence Orthology
more info
 
part_of RISC-loading complex ISO
Inferred from Sequence Orthology
more info
 
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
 
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
 
is_active_in cytoplasmic ribonucleoprotein granule IBA
Inferred from Biological aspect of Ancestor
more info
 
located_in cytoplasmic ribonucleoprotein granule ISO
Inferred from Sequence Orthology
more info
 
located_in cytosol ISO
Inferred from Sequence Orthology
more info
 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
 
located_in nucleus IDA
Inferred from Direct Assay
more info
PubMed 
part_of ribonucleoprotein complex IEA
Inferred from Electronic Annotation
more info
 

General protein information

Preferred Names
protein argonaute-4
Names
Piwi/Argonaute family protein meIF2C4
argonaute 4
argonaute RISC catalytic component 4
eukaryotic translation initiation factor 2C, 4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_153177.3NP_694817.2  protein argonaute-4

    See identical proteins and their annotated locations for NP_694817.2

    Status: VALIDATED

    Source sequence(s)
    AI481660, AK017827, AV306945, BB616446, BC096023
    Consensus CDS
    CCDS18654.1
    UniProtKB/Swiss-Prot
    Q4VBD7, Q6ZPM6, Q8BTF4, Q8CJF8
    Related
    ENSMUSP00000081312.5, ENSMUST00000084289.5
    Conserved Domains (5) summary
    PLN03202
    Location:15860
    PLN03202; protein argonaute; Provisional
    cd02846
    Location:217337
    PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
    cd04657
    Location:384819
    Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...
    pfam08699
    Location:167216
    ArgoL1; Argonaute linker 1 domain
    pfam16486
    Location:18155
    ArgoN; N-terminal domain of argonaute

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000070.7 Reference GRCm39 C57BL/6J

    Range
    126383280..126427379 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006503481.5XP_006503544.1  protein argonaute-4 isoform X2

    Conserved Domains (2) summary
    PLN03202
    Location:15718
    PLN03202; protein argonaute; Provisional
    cd04657
    Location:384739
    Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...
  2. XM_036164538.1XP_036020431.1  protein argonaute-4 isoform X5

    UniProtKB/TrEMBL
    Q8BTF2
    Conserved Domains (1) summary
    cd04657
    Location:5363
    Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...
  3. XM_006503483.5XP_006503546.1  protein argonaute-4 isoform X4

    Conserved Domains (2) summary
    cd04657
    Location:91526
    Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...
    pfam02170
    Location:261
    PAZ; PAZ domain
  4. XM_036164539.1XP_036020432.1  protein argonaute-4 isoform X5

    UniProtKB/TrEMBL
    Q8BTF2
    Conserved Domains (1) summary
    cd04657
    Location:5363
    Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...
  5. XM_036164537.1XP_036020430.1  protein argonaute-4 isoform X1

    Conserved Domains (4) summary
    cd02846
    Location:217337
    PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
    cd04657
    Location:384759
    Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...
    pfam08699
    Location:166216
    ArgoL1; Argonaute linker 1 domain
    pfam16486
    Location:26155
    ArgoN; N-terminal domain of argonaute
  6. XM_006503482.4XP_006503545.1  protein argonaute-4 isoform X3

    Conserved Domains (2) summary
    PLN03202
    Location:15682
    PLN03202; protein argonaute; Provisional
    cl00628
    Location:384682
    Piwi-like; PIWI domain. Domain found in proteins involved in RNA silencing. RNA silencing refers to a group of related gene-silencing mechanisms mediated by short RNA molecules, including siRNAs, miRNAs, and heterochromatin-related guide RNAs. The central component ...