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GORASP1 golgi reassembly stacking protein 1 [ Homo sapiens (human) ]

Gene ID: 64689, updated on 11-Sep-2019

Summary

Official Symbol
GORASP1provided by HGNC
Official Full Name
golgi reassembly stacking protein 1provided by HGNC
Primary source
HGNC:HGNC:16769
See related
Ensembl:ENSG00000114745 MIM:606867
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
P65; GOLPH5; GRASP65
Summary
The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
Expression
Ubiquitous expression in testis (RPKM 12.9), brain (RPKM 12.1) and 25 other tissues See more
Orthologs

Genomic context

See GORASP1 in Genome Data Viewer
Location:
3p22.2
Exon count:
11
Annotation release Status Assembly Chr Location
109.20190905 current GRCh38.p13 (GCF_000001405.39) 3 NC_000003.12 (39096599..39108363, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 3 NC_000003.11 (39138090..39149854, complement)

Chromosome 3 - NC_000003.12Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC107986042 Neighboring gene sodium voltage-gated channel alpha subunit 11 Neighboring gene RNA, U6 small nuclear 1227, pseudogene Neighboring gene WD repeat domain 48 Neighboring gene tetratricopeptide repeat domain 21A Neighboring gene uncharacterized LOC101928263 Neighboring gene microRNA 6822 Neighboring gene cysteine and serine rich nuclear protein 1

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Phenotypes

NHGRI GWAS Catalog

Description
Variability in the common genetic architecture of social-communication spectrum phenotypes during childhood and adolescence.
NHGRI GWA Catalog

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Nef nef HIV-1 Nef causes the FMS N-glycosylation defect and induces relocalization of the GM130 by activating the p56Hck/MEK/ERK/GRASP65 phosphorylation cascade in the Golgi PubMed

Go to the HIV-1, Human Interaction Database

Pathways from BioSystems

  • Asparagine N-linked glycosylation, organism-specific biosystem (from REACTOME)
    Asparagine N-linked glycosylation, organism-specific biosystemN-linked glycosylation is the most important form of post-translational modification for proteins synthesized and folded in the Endoplasmic Reticulum (Stanley et al. 2009). An early study in 1999 rev...
  • COPI-mediated anterograde transport, organism-specific biosystem (from REACTOME)
    COPI-mediated anterograde transport, organism-specific biosystemThe ERGIC (ER-to-Golgi intermediate compartment, also known as vesicular-tubular clusters, VTCs) is a stable, biochemically distinct compartment located adjacent to ER exit sites (Ben-Tekaya et al, 2...
  • COPII (Coat Protein 2) Mediated Vesicle Transport, organism-specific biosystem (from REACTOME)
    COPII (Coat Protein 2) Mediated Vesicle Transport, organism-specific biosystemCOPII components (known as Sec13p, Sec23p, Sec24p, Sec31p, and Sar1p in yeast) traffic cargo from the endoplasmic reticulum to the ER-Golgi intermediate compartment (ERGIC). COPII-coated vesicles wer...
  • Cell Cycle, organism-specific biosystem (from REACTOME)
    Cell Cycle, organism-specific biosystem
    Cell Cycle
  • Cell Cycle, Mitotic, organism-specific biosystem (from REACTOME)
    Cell Cycle, Mitotic, organism-specific biosystemThe replication of the genome and the subsequent segregation of chromosomes into daughter cells are controlled by a series of events collectively known as the cell cycle. DNA replication is carried o...
  • ER to Golgi Anterograde Transport, organism-specific biosystem (from REACTOME)
    ER to Golgi Anterograde Transport, organism-specific biosystemSecretory cargo destined to be secreted or to arrive at the plasma membrane (PM) leaves the ER via distinct exit sites. This cargo is destined for the Golgi apparatus for further processing.
  • Golgi Cisternae Pericentriolar Stack Reorganization, organism-specific biosystem (from REACTOME)
    Golgi Cisternae Pericentriolar Stack Reorganization, organism-specific biosystemThe pericentriolar stacks of Golgi cisternae undergo extensive fragmentation and reorganization in mitosis. In mammalian cells, Golgi apparatus consists of stacked cisternae that are connected by tu...
  • M Phase, organism-specific biosystem (from REACTOME)
    M Phase, organism-specific biosystemMitosis, or the M phase, involves nuclear division and cytokinesis, where two identical daughter cells are produced. Mitosis involves prophase, prometaphase, metaphase, anaphase, and telophase. Fin...
  • Membrane Trafficking, organism-specific biosystem (from REACTOME)
    Membrane Trafficking, organism-specific biosystemThe secretory membrane system allows a cell to regulate delivery of newly synthesized proteins, carbohydrates, and lipids to the cell surface, a necessity for growth and homeostasis. The system is ma...
  • Metabolism of proteins, organism-specific biosystem (from REACTOME)
    Metabolism of proteins, organism-specific biosystemProtein metabolism comprises the pathways of translation, post-translational modification and protein folding.
  • Mitotic Prophase, organism-specific biosystem (from REACTOME)
    Mitotic Prophase, organism-specific biosystemDuring prophase, the chromatin in the nucleus condenses, and the nucleolus disappears. Centrioles begin moving to the opposite poles or sides of the cell. Some of the fibers that extend from the cen...
  • PLK1 signaling events, organism-specific biosystem (from Pathway Interaction Database)
    PLK1 signaling events, organism-specific biosystem
    PLK1 signaling events
  • Post-translational protein modification, organism-specific biosystem (from REACTOME)
    Post-translational protein modification, organism-specific biosystemAfter translation, many newly formed proteins undergo further covalent modifications that alter their functional properties and that are essentially irreversible under physiological conditions in the...
  • Transport to the Golgi and subsequent modification, organism-specific biosystem (from REACTOME)
    Transport to the Golgi and subsequent modification, organism-specific biosystemAt least two mechanisms of transport of proteins from the ER to the Golgi have been described. One is a general flow requiring no export signals (Wieland et al, 1987; Martinez-Menarguez et al, 1999)....
  • Vesicle-mediated transport, organism-specific biosystem (from REACTOME)
    Vesicle-mediated transport, organism-specific biosystemThe transit of proteins and other cargo through the cell requires a cellular transport process in which transported substances are moved in membrane-bounded vesicles. Transported substances are enclo...

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • FLJ23443, MGC118894, MGC118897

Gene Ontology Provided by GOA

Function Evidence Code Pubs
metal ion binding IEA
Inferred from Electronic Annotation
more info
 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
Process Evidence Code Pubs
COPII vesicle coating TAS
Traceable Author Statement
more info
 
ER to Golgi vesicle-mediated transport TAS
Traceable Author Statement
more info
 
Golgi organization IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
Golgi organization ISS
Inferred from Sequence or Structural Similarity
more info
 
establishment of protein localization to plasma membrane IMP
Inferred from Mutant Phenotype
more info
PubMed 
negative regulation of dendrite morphogenesis IMP
Inferred from Mutant Phenotype
more info
PubMed 
positive regulation of ubiquitin protein ligase activity TAS
Traceable Author Statement
more info
 
protein N-linked glycosylation IEA
Inferred from Electronic Annotation
more info
 
protein transport IEA
Inferred from Electronic Annotation
more info
 
Component Evidence Code Pubs
Golgi apparatus IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
Golgi apparatus IDA
Inferred from Direct Assay
more info
PubMed 
Golgi membrane TAS
Traceable Author Statement
more info
 
endoplasmic reticulum-Golgi intermediate compartment membrane TAS
Traceable Author Statement
more info
 

General protein information

Preferred Names
Golgi reassembly-stacking protein 1
Names
Golgi peripheral membrane protein p65
Golgi phosphoprotein 5
Golgi reassembly and stacking protein 1
golgi reassembly stacking protein 1, 65kDa
golgi reassembly-stacking protein of 65 kDa

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001278789.2NP_001265718.1  Golgi reassembly-stacking protein 1 isoform 2

    See identical proteins and their annotated locations for NP_001265718.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) lacks two alternate in-frame exons in the 5' coding region, compared to variant 1. It encodes isoform 2, which lacks an internal segment and is shorter than isoform 1.
    Source sequence(s)
    AI824080, AK001574, AK057043, AK124755, AK303847
    Consensus CDS
    CCDS63597.1
    UniProtKB/Swiss-Prot
    Q9BQQ3
    UniProtKB/TrEMBL
    B3KPY8, B4E1H8
    Related
    ENSP00000419123.1, ENST00000479927.5
    Conserved Domains (2) summary
    cl00117
    Location:3110
    PDZ; PDZ domain, also called DHR (Dlg homologous region) or GLGF (after a conserved sequence motif). Many PDZ domains bind C-terminal polypeptides, though binding to internal (non-C-terminal) polypeptides and even to lipids has been demonstrated. ...
    cl27544
    Location:99203
    Herpes_glycop_D; Herpesvirus glycoprotein D/GG/GX domain
  2. NM_001278790.2NP_001265719.1  Golgi reassembly-stacking protein 1 isoform 3

    See identical proteins and their annotated locations for NP_001265719.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) lacks three alternate exons and uses an alternate splice site in the 5' coding region, compared to variant 1. It encodes isoform 3, which lacks two internal segments and is shorter than isoform 1.
    Source sequence(s)
    AI824080, AK057043, BC008928
    Consensus CDS
    CCDS63596.1
    UniProtKB/Swiss-Prot
    Q9BQQ3
    UniProtKB/TrEMBL
    B3KPY8
    Related
    ENSP00000395709.2, ENST00000422110.6
    Conserved Domains (1) summary
    cl00117
    Location:350
    PDZ; PDZ domain, also called DHR (Dlg homologous region) or GLGF (after a conserved sequence motif). Many PDZ domains bind C-terminal polypeptides, though binding to internal (non-C-terminal) polypeptides and even to lipids has been demonstrated. ...
  3. NM_031899.3NP_114105.1  Golgi reassembly-stacking protein 1 isoform 1

    See identical proteins and their annotated locations for NP_114105.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes the longest isoform (1).
    Source sequence(s)
    AI824080, AK001574, AK057043, AK124755
    Consensus CDS
    CCDS2681.1
    UniProtKB/Swiss-Prot
    Q9BQQ3
    UniProtKB/TrEMBL
    A0A024R2U5, B3KPY8
    Related
    ENSP00000313869.3, ENST00000319283.7
    Conserved Domains (3) summary
    pfam04495
    Location:69205
    GRASP55_65; GRASP55/65 PDZ-like domain
    cl00117
    Location:3100
    PDZ; PDZ domain, also called DHR (Dlg homologous region) or GLGF (after a conserved sequence motif). Many PDZ domains bind C-terminal polypeptides, though binding to internal (non-C-terminal) polypeptides and even to lipids has been demonstrated. ...
    cl27544
    Location:194298
    Herpes_glycop_D; Herpesvirus glycoprotein D/GG/GX domain

RNA

  1. NR_103866.2 RNA Sequence

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) uses an alternate splice site in an internal exon, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
    Source sequence(s)
    AI824080, AK124755, BC008928, BM470731, DB031221
    Related
    ENST00000452389.6
  2. NR_103867.2 RNA Sequence

    Status: REVIEWED

    Description
    Transcript Variant: This variant (5) uses an alternate splice site in an internal exon, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
    Source sequence(s)
    AI824080, AK057043, BC064624
    Related
    ENST00000453680.5

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000003.12 Reference GRCh38.p13 Primary Assembly

    Range
    39096599..39108363 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006713301.4XP_006713364.1  Golgi reassembly-stacking protein 1 isoform X1

    Conserved Domains (2) summary
    pfam04495
    Location:74210
    GRASP55_65; GRASP55/65 PDZ-like domain
    cl00117
    Location:26105
    PDZ; PDZ domain, also called DHR (Dlg homologous region) or GLGF (after a conserved sequence motif). Many PDZ domains bind C-terminal polypeptides, though binding to internal (non-C-terminal) polypeptides and even to lipids has been demonstrated. ...
  2. XM_017007051.2XP_016862540.1  Golgi reassembly-stacking protein 1 isoform X5

    Conserved Domains (1) summary
    cl00117
    Location:69145
    PDZ; PDZ domain, also called DHR (Dlg homologous region) or GLGF (after a conserved sequence motif). Many PDZ domains bind C-terminal polypeptides, though binding to internal (non-C-terminal) polypeptides and even to lipids has been demonstrated. ...
  3. XM_017007050.2XP_016862539.1  Golgi reassembly-stacking protein 1 isoform X3

    Conserved Domains (2) summary
    pfam04495
    Location:69175
    GRASP55_65; GRASP55/65 PDZ-like domain
    cl00117
    Location:3100
    PDZ; PDZ domain, also called DHR (Dlg homologous region) or GLGF (after a conserved sequence motif). Many PDZ domains bind C-terminal polypeptides, though binding to internal (non-C-terminal) polypeptides and even to lipids has been demonstrated. ...
  4. XM_017007052.2XP_016862541.1  Golgi reassembly-stacking protein 1 isoform X6

  5. XM_024453714.1XP_024309482.1  Golgi reassembly-stacking protein 1 isoform X7

    Conserved Domains (2) summary
    cl00117
    Location:635
    PDZ; PDZ domain, also called DHR (Dlg homologous region) or GLGF (after a conserved sequence motif). Many PDZ domains bind C-terminal polypeptides, though binding to internal (non-C-terminal) polypeptides and even to lipids has been demonstrated. ...
    cl27544
    Location:36128
    Herpes_glycop_D; Herpesvirus glycoprotein D/GG/GX domain
  6. XM_011534021.3XP_011532323.1  Golgi reassembly-stacking protein 1 isoform X4

    Conserved Domains (1) summary
    cl00117
    Location:74150
    PDZ; PDZ domain, also called DHR (Dlg homologous region) or GLGF (after a conserved sequence motif). Many PDZ domains bind C-terminal polypeptides, though binding to internal (non-C-terminal) polypeptides and even to lipids has been demonstrated. ...
  7. XM_011534020.3XP_011532322.1  Golgi reassembly-stacking protein 1 isoform X2

    Conserved Domains (2) summary
    pfam04495
    Location:74180
    GRASP55_65; GRASP55/65 PDZ-like domain
    cl00117
    Location:26105
    PDZ; PDZ domain, also called DHR (Dlg homologous region) or GLGF (after a conserved sequence motif). Many PDZ domains bind C-terminal polypeptides, though binding to internal (non-C-terminal) polypeptides and even to lipids has been demonstrated. ...

RNA

  1. XR_940493.3 RNA Sequence

  2. XR_002959572.1 RNA Sequence

  3. XR_002959570.1 RNA Sequence

  4. XR_002959573.1 RNA Sequence

  5. XR_002959571.1 RNA Sequence

  6. XR_002959569.1 RNA Sequence

  7. XR_940494.3 RNA Sequence

  8. XR_001740235.2 RNA Sequence

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