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DPF2 double PHD fingers 2 [ Homo sapiens (human) ]

Gene ID: 5977, updated on 13-Mar-2020

Summary

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Official Symbol
DPF2provided by HGNC
Official Full Name
double PHD fingers 2provided by HGNC
Primary source
HGNC:HGNC:9964
See related
Ensembl:ENSG00000133884 MIM:601671
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
REQ; CSS7; UBID4; ubi-d4
Summary
The protein encoded by this gene is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It likely serves a regulatory role in rapid hematopoietic cell growth and turnover. This gene is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors. [provided by RefSeq, Jul 2008]
Expression
Ubiquitous expression in testis (RPKM 23.7), ovary (RPKM 23.0) and 25 other tissues See more
Orthologs
mouse all

Genomic context

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See DPF2 in Genome Data Viewer
Location:
11q13.1
Exon count:
12
Annotation release Status Assembly Chr Location
109.20200228 current GRCh38.p13 (GCF_000001405.39) 11 NC_000011.10 (65333840..65354262)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 11 NC_000011.9 (65101225..65120451)

Chromosome 11 - NC_000011.10Genomic Context describing neighboring genes Neighboring gene DNA polymerase alpha 2, accessory subunit Neighboring gene uncharacterized LOC105369344 Neighboring gene CDC42 effector protein 2 Neighboring gene tigger transposable element derived 3 Neighboring gene Sharpr-MPRA regulatory region 983 Neighboring gene solute carrier family 25 member 45

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. Crystal structure of the Cys2His2-type zinc finger domain of human DPF2. Zhang W, et al. Biochem Biophys Res Commun, 2011 Sep 16. PMID 21888896
  2. Double Plant Homeodomain Fingers 2 (DPF2) Promotes the Immune Escape of Influenza Virus by Suppressing Beta Interferon Production. Shin D, et al. J Virol, 2017 Jun 15. PMID 28404846, Free PMC Article
  3. DPF2 regulates OCT4 protein level and nuclear distribution. Liu C, et al. Biochim Biophys Acta, 2015 Dec. PMID 26417682
  4. Simultaneous targeting of Requiem & Alg-2 in Chinese hamster ovary cells for improved recombinant protein production. Lim Y, et al. Mol Biotechnol, 2010 Nov. PMID 20571937
  5. Requiem: a novel zinc finger gene essential for apoptosis in myeloid cells. Gabig TG, et al. J Biol Chem, 1994 Nov 25. PMID 7961935

See all (58) citations in PubMed

See citations in PubMed for homologs of this gene provided by HomoloGene

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. de novo variants in DPF2 cause Coffin-Siris syndrome and propose a dominant-negative mechanism of pathogenicity.
    Title: Mutations in the BAF-Complex Subunit DPF2 Are Associated with Coffin-Siris Syndrome.
  2. Authors show that DPF2 interacts with the acetylated tails of both histones 3 and 4 via bipartite binding pockets on the DPF2 surface. Blocking these interactions through targeted mutagenesis of DPF2 abolishes its recruitment to target chromatin regions as well as its ability to prevent myeloid differentiation in vivo.
    Title: Histone-binding of DPF2 mediates its repressive role in myeloid differentiation.
  3. Upon influenza virus infection, DPF2 dysregulated IFN-beta induction and expression of cytokines/chemokines and antiviral proteins. This study provides evidence that influenza virus utilizes DPF2 to escape host innate immunity.
    Title: Double Plant Homeodomain Fingers 2 (DPF2) Promotes the Immune Escape of Influenza Virus by Suppressing Beta Interferon Production.
  4. The demonstrate that the histone acetylation-binding double PHD finger (DPF) domains of human MOZ (also known as KAT6A) and DPF2 (also known as BAF45d) accommodate a wide range of histone lysine acylation with the strongest preference for propionylation (Kcr).
    Title: Selective recognition of histone crotonylation by double PHD fingers of MOZ and DPF2.
  5. the role of SMARCA4 and the two SWI/SNF subunits SMARCD2/BAF60B and DPF2/BAF45D in leukaemia, was investigated.
    Title: SWI/SNF Subunits SMARCA4, SMARCD2 and DPF2 Collaborate in MLL-Rearranged Leukaemia Maintenance.
  6. DPF2 decreases monomeric and mono-ubiquitinated OCT4, assembles poly-ubiquitin chains on OCT4 mainly through Ub-K48 linkage
    Title: DPF2 regulates OCT4 protein level and nuclear distribution.
  7. crystal structure of the C2H2-type zinc finger domain of human DPF2 with a canonical C2H2 fold, which contains two beta strands and one alpha helix, was reported.
    Title: Crystal structure of the Cys2His2-type zinc finger domain of human DPF2.
  8. found that targeting Requiem and Alg-2 did not result in extended culture viability, but resulted in an increase in maximum viable cell numbers and cumulative IVCD under fed-batch conditions
    Title: Simultaneous targeting of Requiem & Alg-2 in Chinese hamster ovary cells for improved recombinant protein production.
  9. REQ functions as an efficient adaptor protein between the SWI/SNF complex and RelB/p52 and plays important roles in noncanonical NF-kappaB transcriptional activation and its associated oncogenic activity.
    Title: Requiem protein links RelB/p52 and the Brm-type SWI/SNF complex in a noncanonical NF-kappaB pathway.
Submit: New GeneRIF Correction

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Associated conditions

Description Tests
COFFIN-SIRIS SYNDROME 7
MedGen: C4747954 OMIM: 618027 GeneReviews: Not available
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NHGRI GWAS Catalog

Description
Genome-wide association study identifies three novel susceptibility loci for severe Acne vulgaris.
NHGRI GWA Catalog

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • MGC10180

Gene Ontology Provided by GOA

Function Evidence Code Pubs
H3K9me3 modified histone binding IDA
Inferred from Direct Assay
more info
 PubMed 
RNA polymerase II proximal promoter sequence-specific DNA binding HDA PubMed 
histone acetyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
histone binding IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
lysine-acetylated histone binding IDA
Inferred from Direct Assay
more info
 PubMed 
metal ion binding IEA
Inferred from Electronic Annotation
more info
  
protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
transcription corepressor activity TAS
Traceable Author Statement
more info
 PubMed 
Process Evidence Code Pubs
apoptotic process TAS
Traceable Author Statement
more info
 PubMed 
apoptotic signaling pathway TAS
Traceable Author Statement
more info
 PubMed 
histone acetylation IEA
Inferred from Electronic Annotation
more info
  
negative regulation of myeloid progenitor cell differentiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
negative regulation of transcription by RNA polymerase II TAS
Traceable Author Statement
more info
 PubMed 
negative regulation of transcription, DNA-templated IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
nervous system development IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
positive regulation of transcription by RNA polymerase II IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
Component Evidence Code Pubs
centrosome IDA
Inferred from Direct Assay
more info
  
cytosol IDA
Inferred from Direct Assay
more info
  
histone acetyltransferase complex IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
intracellular membrane-bounded organelle IDA
Inferred from Direct Assay
more info
  
nBAF complex IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
nuclear chromatin HDA PubMed 
nuclear chromatin IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
nuclear chromatin IDA
Inferred from Direct Assay
more info
 PubMed 
nucleoplasm IDA
Inferred from Direct Assay
more info
  
nucleus IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
nucleus IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
zinc finger protein ubi-d4
Names
BAF45D
BRG1-associated factor 45D
D4, zinc and double PHD fingers family 2
apoptosis response zinc finger protein
protein requiem
requiem, apoptosis response zinc finger

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001330308.2 → NP_001317237.1  zinc finger protein ubi-d4 isoform 2

    Status: REVIEWED

    Source sequence(s)
    AI381588, AL555427, AP000944, BI753338, BP201169, CB045170
    Consensus CDS
    CCDS81583.1
    UniProtKB/TrEMBL
    J3KMZ8
    Related
    ENSP00000252268.4, ENST00000252268.8
    Conserved Domains (4) summary
    COG5189
    Location:201 → 247
    SFP1; Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division and chromosome partitioning]
    cd15530
    Location:343 → 388
    PHD2_d4; PHD finger 2 found in d4 gene family proteins
    cd15691
    Location:286 → 341
    PHD1_DPF2_like; PHD finger 1 found in D4, zinc and double PHD fingers family 2 (DPF2)
    pfam14051
    Location:13 → 79
    Requiem_N; N-terminal domain of DPF2/REQ

  2. NM_006268.5 → NP_006259.1  zinc finger protein ubi-d4 isoform 1

    See identical proteins and their annotated locations for NP_006259.1

    Status: REVIEWED

    Source sequence(s)
    AK313408, AP000944, BC014889
    Consensus CDS
    CCDS8100.1
    UniProtKB/Swiss-Prot
    Q92785
    UniProtKB/TrEMBL
    A0A024R582
    Related
    ENSP00000436901.1, ENST00000528416.6
    Conserved Domains (4) summary
    COG5189
    Location:201 → 233
    SFP1; Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division and chromosome partitioning]
    cd15530
    Location:329 → 374
    PHD2_d4; PHD finger 2 found in d4 gene family proteins
    cd15691
    Location:272 → 327
    PHD1_DPF2_like; PHD finger 1 found in D4, zinc and double PHD fingers family 2 (DPF2)
    pfam14051
    Location:13 → 79
    Requiem_N; N-terminal domain of DPF2/REQ

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000011.10 Reference GRCh38.p13 Primary Assembly

    Range
    65333840..65354262
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_024448637.1 → XP_024304405.1  zinc finger protein ubi-d4 isoform X1

    Conserved Domains (4) summary
    COG5189
    Location:397 → 417
    SFP1; Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division and chromosome partitioning]
    cd15530
    Location:513 → 558
    PHD2_d4; PHD finger 2 found in d4 gene family proteins
    cd15691
    Location:456 → 511
    PHD1_DPF2_like; PHD finger 1 found in D4, zinc and double PHD fingers family 2 (DPF2)
    pfam14051
    Location:13 → 79
    Requiem_N; N-terminal domain of DPF2/REQ

  2. XM_024448638.1 → XP_024304406.1  zinc finger protein ubi-d4 isoform X2

    Conserved Domains (4) summary
    COG5189
    Location:377 → 397
    SFP1; Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division and chromosome partitioning]
    cd15530
    Location:493 → 538
    PHD2_d4; PHD finger 2 found in d4 gene family proteins
    cd15691
    Location:436 → 491
    PHD1_DPF2_like; PHD finger 1 found in D4, zinc and double PHD fingers family 2 (DPF2)
    pfam14051
    Location:1 → 59
    Requiem_N; N-terminal domain of DPF2/REQ

  3. XM_017018101.2 → XP_016873590.1  zinc finger protein ubi-d4 isoform X3

    Conserved Domains (4) summary
    COG5189
    Location:181 → 227
    SFP1; Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division and chromosome partitioning]
    cd15530
    Location:323 → 368
    PHD2_d4; PHD finger 2 found in d4 gene family proteins
    cd15691
    Location:266 → 321
    PHD1_DPF2_like; PHD finger 1 found in D4, zinc and double PHD fingers family 2 (DPF2)
    pfam14051
    Location:1 → 59
    Requiem_N; N-terminal domain of DPF2/REQ

  4. XM_017018102.1 → XP_016873591.1  zinc finger protein ubi-d4 isoform X4

    Conserved Domains (3) summary
    cd15530
    Location:309 → 354
    PHD2_d4; PHD finger 2 found in d4 gene family proteins
    cd15691
    Location:252 → 307
    PHD1_DPF2_like; PHD finger 1 found in D4, zinc and double PHD fingers family 2 (DPF2)
    pfam14051
    Location:1 → 59
    Requiem_N; N-terminal domain of DPF2/REQ

RNA

  1. XR_950008.3 RNA Sequence

Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q92785.2 GenPept UniProtKB/Swiss-Prot:Q92785

Gene LinkOut

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