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PURA purine rich element binding protein A [ Homo sapiens (human) ]

Gene ID: 5813, updated on 7-Oct-2018

Summary

Official Symbol
PURAprovided by HGNC
Official Full Name
purine rich element binding protein Aprovided by HGNC
Primary source
HGNC:HGNC:9701
See related
Ensembl:ENSG00000185129 MIM:600473; Vega:OTTHUMG00000129242
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
PUR1; MRD31; PURALPHA; PUR-ALPHA
Summary
This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. [provided by RefSeq, Jul 2008]
Orthologs

Genomic context

See PURA in Genome Data Viewer
Location:
5q31.3
Exon count:
1
Annotation release Status Assembly Chr Location
109 current GRCh38.p12 (GCF_000001405.38) 5 NC_000005.10 (140114123..140119416)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 5 NC_000005.9 (139493708..139499001)

Chromosome 5 - NC_000005.10Genomic Context describing neighboring genes Neighboring gene neuregulin 2 Neighboring gene mitosis associated long intergenic non-coding RNA 1 Neighboring gene uncharacterized LOC101929719 Neighboring gene IgA inducing protein

Genomic regions, transcripts, and products

Bibliography

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

Phenotypes

Copy number response

Description
Copy number response
Triplosensitivity

No evidence available (Last evaluated (2017-06-07)

ClinGen Genome Curation Page
Haploinsufficency

Sufficient evidence for dosage pathogenicity (Last evaluated (2017-06-07)

ClinGen Genome Curation PagePubMed

HIV-1 interactions

Replication interactions

Interaction Pubs
Knockdown of purine-rich element binding protein A (PURA) by siRNA inhibits HIV-1 replication in HeLa-derived TZM-bl cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Rev rev The interaction of Puralpha with HIV-1 RRE RNA occurs in the cytoplasm and enhances the association of HIV-1 Rev with the RRE PubMed
rev HIV-1 Rev and PURA colocalize in the perinuclear compartment. The 18-50 amino-acid residues of Rev interact with the region of amino acids 73-123 in PURA PubMed
Tat tat HIV-1 Tat proteins from different clades modulate differently Pur-alpha and SMAD4 to bind the JCV non-coding control region and affect differently on SMAD4 localization to the nucleus PubMed
tat Puralpha interacts with HIV-1 Tat to regulate the effect of Tat transactivating the HIV-1 LTR promoter, as well as the JCV promoter and other cellular promoters PubMed
tat Puralpha self-associates in an RNA dependent manner, an effect that is inhibited by HIV-1 Tat PubMed
tat Puralpha-mediated inhibition of protein translation can be inhibited by HIV-1 Tat PubMed
tat HIV-1 Tat cooperates with Puralpha, cyclin T1, and CDK9 to activate the TNFalpha promoter and upregulate TNFalpha expression PubMed
tat Expression of HIV-1 Tat in human SK-N-MC cells inhibits the NGF-signaling pathway leading to suppression of MAPK and reduction of Puralpha binding to the Egr-1 promoter PubMed
tat Binding of HIV-1 Tat to Puralpha is mediated through amino acids 49-72 of Tat and 100-121 of Puralpha, and is enhanced by RNA PubMed
tat HIV-1 Tat downregulates the expression of p35, a neuron-specific activator of cdk5, and also binds to Puralpha, which associates with cdk5, leading to deregulation of neuronal differentiation and survival PubMed

Go to the HIV-1, Human Interaction Database

Pathways from BioSystems

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
DNA-binding transcription factor activity, RNA polymerase II-specific ISA
Inferred from Sequence Alignment
more info
 
DNA-binding transcription repressor activity, RNA polymerase II-specific IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
RNA binding HDA PubMed 
RNA polymerase II regulatory region sequence-specific DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
SMAD binding IEA
Inferred from Electronic Annotation
more info
 
double-stranded DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
double-stranded telomeric DNA binding IDA
Inferred from Direct Assay
more info
PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
purine-rich negative regulatory element binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
sequence-specific DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
single-stranded DNA binding ISS
Inferred from Sequence or Structural Similarity
more info
 
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding TAS
Traceable Author Statement
more info
PubMed 
transcription factor binding ISS
Inferred from Sequence or Structural Similarity
more info
 
translation repressor activity, mRNA regulatory element binding IEA
Inferred from Electronic Annotation
more info
 
Process Evidence Code Pubs
DNA replication initiation TAS
Traceable Author Statement
more info
PubMed 
DNA unwinding involved in DNA replication IDA
Inferred from Direct Assay
more info
PubMed 
cell differentiation IEA
Inferred from Electronic Annotation
more info
 
dendritic transport of messenger ribonucleoprotein complex IEA
Inferred from Electronic Annotation
more info
 
negative regulation of transcription by RNA polymerase II IEA
Inferred from Electronic Annotation
more info
 
negative regulation of translation IEA
Inferred from Electronic Annotation
more info
 
nervous system development IEA
Inferred from Electronic Annotation
more info
 
positive regulation of cell proliferation IEA
Inferred from Electronic Annotation
more info
 
Component Evidence Code Pubs
DNA replication factor A complex ISS
Inferred from Sequence or Structural Similarity
more info
 
dendrite cytoplasm IEA
Inferred from Electronic Annotation
more info
 
glutamatergic synapse IEA
Inferred from Electronic Annotation
more info
 
neuronal cell body IEA
Inferred from Electronic Annotation
more info
 
nuclear chromosome, telomeric region IC
Inferred by Curator
more info
PubMed 
nucleus IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
nucleus IDA
Inferred from Direct Assay
more info
PubMed 
postsynapse IEA
Inferred from Electronic Annotation
more info
 

General protein information

Preferred Names
transcriptional activator protein Pur-alpha
Names
purine-rich single-stranded DNA-binding protein alpha

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_041813.1 RefSeqGene

    Range
    5001..10294
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_005859.4NP_005850.1  transcriptional activator protein Pur-alpha

    See identical proteins and their annotated locations for NP_005850.1

    Status: REVIEWED

    Source sequence(s)
    AC011379, CK819077, M96684
    Consensus CDS
    CCDS4220.1
    UniProtKB/Swiss-Prot
    Q00577
    Related
    ENSP00000332706.3, OTTHUMP00000159547, ENST00000331327.4, OTTHUMT00000251341
    Conserved Domains (1) summary
    pfam04845
    Location:58278
    PurA; PurA ssDNA and RNA-binding protein

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p12 Primary Assembly

Genomic

  1. NC_000005.10 Reference GRCh38.p12 Primary Assembly

    Range
    140114123..140119416
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
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