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PRSS3 serine protease 3 [ Homo sapiens (human) ]

Gene ID: 5646, updated on 3-Nov-2024

Summary

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Official Symbol
PRSS3provided by HGNC
Official Full Name
serine protease 3provided by HGNC
Primary source
HGNC:HGNC:9486
See related
Ensembl:ENSG00000010438 MIM:613578; AllianceGenome:HGNC:9486
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
T9; MTG; TRY3; TRY4; PRSS4
Summary
This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide linkages involving the carboxyl group of lysine or arginine. This gene is localized to the locus of T cell receptor beta variable orphans on chromosome 9. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2010]
Expression
Restricted expression toward pancreas (RPKM 3091.3) See more
Orthologs
all
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Genomic context

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See PRSS3 in Genome Data Viewer
Location:
9p13.3
Exon count:
8
Annotation release Status Assembly Chr Location
RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 9 NC_000009.12 (33750679..33799231)
RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 9 NC_060933.1 (33769262..33817820)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 9 NC_000009.11 (33750677..33799229)

Chromosome 9 - NC_000009.12Genomic Context describing neighboring genes Neighboring gene T cell receptor beta variable 26/OR9-2 (pseudogene) Neighboring gene uncharacterized LOC124902143 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 28300 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr9:33721507-33722008 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr9:33722009-33722508 Neighboring gene UBE2R2 antisense RNA 1 Neighboring gene MPRA-validated peak7221 silencer Neighboring gene ATAC-STARR-seq lymphoblastoid active region 28301 Neighboring gene T cell receptor beta variable 29/OR9-2 (non-functional) Neighboring gene H3K4me1 hESC enhancer GRCh37_chr9:33796108-33796608 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 28302 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 19835 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 19836 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 19837 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 19838 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 19839 Neighboring gene ubiquitin conjugating enzyme E2 R2 Neighboring gene RNA, U4atac small nuclear 11, pseudogene Neighboring gene ATAC-STARR-seq lymphoblastoid active region 28303 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr9:33927431-33928421 Neighboring gene small nucleolar RNA, C/D box 121B Neighboring gene ubiquitin associated protein 2

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. Nucleotide sequence of the human pancreatic trypsinogen III cDNA. Tani T, et al. Nucleic Acids Res, 1990 Mar 25. PMID 2326201, Free PMC Article
  2. Epigenetic silencing of PRSS3 provides growth and metastasis advantage for human hepatocellular carcinoma. Lin B, et al. J Mol Med (Berl), 2017 Nov. PMID 28844099, Free PMC Article
  3. Small molecule inhibitors of mesotrypsin from a structure-based docking screen. Kayode O, et al. PLoS One, 2017. PMID 28463992, Free PMC Article
  4. PRSS3 is a prognostic marker in invasive ductal carcinoma of the breast. Qian L, et al. Oncotarget, 2017 Mar 28. PMID 28423522, Free PMC Article
  5. Mesotrypsin Has Evolved Four Unique Residues to Cleave Trypsin Inhibitors as Substrates. Alloy AP, et al. J Biol Chem, 2015 Aug 28. PMID 26175157, Free PMC Article

See all (84) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Shear Stress Drives the Cleavage Activation of Protease-Activated Receptor 2 by PRSS3/Mesotrypsin to Promote Invasion and Metastasis of Circulating Lung Cancer Cells.
    Title: Shear Stress Drives the Cleavage Activation of Protease-Activated Receptor 2 by PRSS3/Mesotrypsin to Promote Invasion and Metastasis of Circulating Lung Cancer Cells.
  2. Serine Protease 3 Promotes Progression of Diffuse Large B-Cell Lymphoma and Serves as a Novel Prognostic Predictor.
    Title: Serine Protease 3 Promotes Progression of Diffuse Large B-Cell Lymphoma and Serves as a Novel Prognostic Predictor.
  3. Host neuronal PRSS3 interacts with enterovirus A71 3A protein and its role in viral replication.
    Title: Host neuronal PRSS3 interacts with enterovirus A71 3A protein and its role in viral replication.
  4. In-depth proteomics analysis of sentinel lymph nodes from individuals with endometrial cancer.
    Title: In-depth proteomics analysis of sentinel lymph nodes from individuals with endometrial cancer.
  5. High Expression of PRSS3 Indicates Unfavorable Clinical Outcomes in Colon Adenocarcinoma.
    Title: High Expression of PRSS3 Indicates Unfavorable Clinical Outcomes in Colon Adenocarcinoma.
  6. Defective binding of SPINK1 variants is an uncommon mechanism for impaired trypsin inhibition in chronic pancreatitis.
    Title: Defective binding of SPINK1 variants is an uncommon mechanism for impaired trypsin inhibition in chronic pancreatitis.
  7. Inactivation of mesotrypsin by chymotrypsin C prevents trypsin inhibitor degradation.
    Title: Inactivation of mesotrypsin by chymotrypsin C prevents trypsin inhibitor degradation.
  8. PRSS3/Mesotrypsin and kallikrein-related peptidase 5 are associated with poor prognosis and contribute to tumor cell invasion and growth in lung adenocarcinoma.
    Title: PRSS3/Mesotrypsin and kallikrein-related peptidase 5 are associated with poor prognosis and contribute to tumor cell invasion and growth in lung adenocarcinoma.
  9. PRSS contributes to cetuximab resistance in colorectal cancer.
    Title: PRSS contributes to cetuximab resistance in colorectal cancer.
  10. Circular RNA ADAM9 facilitates the malignant behaviours of pancreatic cancer by sponging miR-217 and upregulating PRSS3 expression
    Title: Circular RNA ADAM9 facilitates the malignant behaviours of pancreatic cancer by sponging miR-217 and upregulating PRSS3 expression.
Submit: New GeneRIF Correction See all GeneRIFs (41)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Collaborative genome-wide association analysis supports a role for ANK3 and CACNA1C in bipolar disorder.
EBI GWAS Catalog

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables calcium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables serine-type endopeptidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables serine-type endopeptidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables serine-type peptidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables serine-type peptidase activity TAS
Traceable Author Statement
more info
  
Process Evidence Code Pubs
involved_in antimicrobial humoral response TAS
Traceable Author Statement
more info
  
involved_in digestion TAS
Traceable Author Statement
more info
 PubMed 
involved_in endothelial cell migration IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in proteolysis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in proteolysis IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in proteolysis NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in zymogen activation IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in extracellular region TAS
Traceable Author Statement
more info
  
is_active_in extracellular space IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in extracellular space IDA
Inferred from Direct Assay
more info
 PubMed 
located_in tertiary granule lumen TAS
Traceable Author Statement
more info
  

General protein information

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Preferred Names
trypsin-3
Names
brain trypsinogen
mesotrypsin
mesotrypsinogen
pancreatic trypsinogen III
protease, serine 3
protease, serine, 4 (trypsin 4, brain)
trypsin III
trypsin IV
trypsinogen 4
trypsinogen 5
trypsinogen IV
NP_001184026.3
NP_001184027.1
NP_002762.3
NP_031369.3
XP_011516267.2
XP_047279558.1
XP_054219277.1
XP_054219278.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_001337.2 

    Range
    144006..192771
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

  2. NG_029635.2 RefSeqGene

    Range
    49884..53554
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001197097.3 → NP_001184026.3  trypsin-3 isoform 3 preproprotein

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) contains an alternate in-frame exon in the 5' coding region and uses a downstream start codon, compared to variant 1. The resulting protein (isoform 3) has a shorter N-terminus, compared to isoform 1.
    Source sequence(s)
    AL139113, AL356489, AL358573
    UniProtKB/TrEMBL
    A0A7P0MNE9
    Related
    ENSP00000340889.5, ENST00000342836.9
    Conserved Domains (1) summary
    cd00190
    Location:38 → 256
    Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...

  2. NM_001197098.1 → NP_001184027.1  trypsin-3 isoform 4 preproprotein

    See identical proteins and their annotated locations for NP_001184027.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) contains a different in-frame first exon and uses an alternate start codon, compared to variant 1. The resulting protein (isoform 4) has a shorter and distinct N-terminus, compared to isoform 1.
    Source sequence(s)
    AB298286
    Consensus CDS
    CCDS56570.1
    UniProtKB/TrEMBL
    Q8N2U3
    Related
    ENSP00000401828.3, ENST00000429677.8
    Conserved Domains (2) summary
    smart00020
    Location:16 → 232
    Tryp_SPc; Trypsin-like serine protease
    cd00190
    Location:17 → 235
    Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...

  3. NM_002771.4 → NP_002762.3  trypsin-3 isoform 2 preproprotein

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region compared to variant 1. The resulting isoform (2) has a distinct N-terminus and is shorter than isoform 1.
    Source sequence(s)
    AL139113, AL358573
    Consensus CDS
    CCDS6545.1
    UniProtKB/TrEMBL
    A1A508
    Related
    ENSP00000368715.3, ENST00000379405.4
    Conserved Domains (1) summary
    cd00190
    Location:24 → 242
    Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...

  4. NM_007343.4 → NP_031369.3  trypsin-3 isoform 1 preproprotein

    Status: REVIEWED

    Source sequence(s)
    AL139113, AL356489, AL358573
    Consensus CDS
    CCDS47958.2
    UniProtKB/TrEMBL
    A0A7P0MP65
    Related
    ENSP00000354280.6, ENST00000361005.10
    Conserved Domains (1) summary
    cd00190
    Location:1 → 134
    Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000009.12 Reference GRCh38.p14 Primary Assembly

    Range
    33750679..33799231
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011517965.2 → XP_011516267.2  trypsin-3 isoform X1

    UniProtKB/TrEMBL
    A0A7P0MP65

  2. XM_047423602.1 → XP_047279558.1  trypsin-3 isoform X2

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060933.1 Alternate T2T-CHM13v2.0

    Range
    33769262..33817820
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054363302.1 → XP_054219277.1  trypsin-3 isoform X2

  2. XM_054363303.1 → XP_054219278.1  trypsin-3 isoform X2

Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P35030.2 GenPept UniProtKB/Swiss-Prot:P35030

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