U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

PRKCI protein kinase C iota [ Homo sapiens (human) ]

Gene ID: 5584, updated on 7-Aug-2022

Summary

Official Symbol
PRKCIprovided by HGNC
Official Full Name
protein kinase C iotaprovided by HGNC
Primary source
HGNC:HGNC:9404
See related
Ensembl:ENSG00000163558 MIM:600539; AllianceGenome:HGNC:9404
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
PKCI; DXS1179E; nPKC-iota
Summary
This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq, Jul 2008]
Expression
Ubiquitous expression in stomach (RPKM 19.1), thyroid (RPKM 17.9) and 24 other tissues See more
Orthologs
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

See PRKCI in Genome Data Viewer
Location:
3q26.2
Exon count:
19
Annotation release Status Assembly Chr Location
110 current GRCh38.p14 (GCF_000001405.40) 3 NC_000003.12 (170222424..170305977)
110 current T2T-CHM13v2.0 (GCF_009914755.1) 3 NC_060927.1 (173007078..173090623)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 3 NC_000003.11 (169940212..170023765)

Chromosome 3 - NC_000003.12Genomic Context describing neighboring genes Neighboring gene polyhomeotic homolog 3 Neighboring gene RNA, U6 small nuclear 315, pseudogene Neighboring gene RNA, U7 small nuclear 32 pseudogene Neighboring gene Sharpr-MPRA regulatory region 5121 Neighboring gene SKI like proto-oncogene

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env HIV-1 gp120 upregulates the expression of protein kinase C, iota (PRKCI) in human B cells PubMed
env HIV-1 gp120 activates forward trafficking and surface clustering of NMDA receptors in membrane micro domains by a PKA-dependent phosphorylation of the NR1 C-terminal Ser897, followed by a PKC-dependent phosphorylation of Ser896 PubMed
env Pre-treatment of endothelial cells with fibroblast growth factor 2 (FGF2) protects cells from HIV-1 gp120 angiotoxicity; this protection is regulated by crosstalk among the ERK, PI3K-AKT and PKC signaling pathways PubMed
env Down modulation of the interaction between HIV-1 gp120 and CD4 by TPA is blocked by protein kinase C (PKC) inhibitors, suggesting PKC may play an important role in HIV-1 infection PubMed
env IL-16 induces rapid translocation of PKC from the cytosol to the membrane in CD4+ cells; PKC inhibitors completely block IL-16-induced lymphocyte migration as well as the motile response induced by HIV-1 gp120 and anti-CD4 antibody binding to CD4 PubMed
env c-FLIPL inhibits Bax activation via modulating PKC expression at the transcriptional level involving AP-2 during gp120 treatment PubMed
env A specific interaction between CD4 and HIV-1 gp120 is required for phosphorylation of CD4, which could involve protein kinase C PubMed
env HIV-1 gp120 increases the levels of calcium-dependent and -independent PKC isozymes; the most striking change is observed in PKC-zeta isozyme levels PubMed
env Induction of apoptosis in cell cultures through binding of HIV-1 gp120 or gp160 to CXCR4 involves protein kinase C, basic fibroblast growth factor, caspase-3, and the pro-apoptotic molecule Bax PubMed
Envelope surface glycoprotein gp160, precursor env HIV-1 gp160-induced AP-1 complex formation is dependent upon protein tyrosine phosphorylation and is abolished by inhibitors of protein kinase C, but it is unaffected by calcium channel blockers or cyclosporine A PubMed
Envelope transmembrane glycoprotein gp41 env A synthetic peptide corresponding to cytoplasmic domain residues 828-848 of HIV-1 gp41 inhibits pKC-catalysed phosphorylation of a protein substrate PubMed
env A synthetic peptide containing residues 581-597 from HIV-1 gp41 inhibits protein kinase C (pkC)-mediated phosphorylation of the CD3 gamma-chain in intact cells and directly inhibits partially purified pkC PubMed
Pr55(Gag) gag The phosphorylation of HIV-1 Gag by PRKCI regulates HIV-1 Vpr incorporation into virions and HIV-1 replication in macrophages PubMed
gag Immunoprecipation analysis demonstrates that PRKCI binds HIV-1 Gag and phosphorylates Gag at position serine 487 in the p6 domain of Gag PubMed
gag The amplified luminescent proximity homogeneous assay (AlphaScreen) identifies the interaction of HIV-1 Gag with PRKCI PubMed
Tat tat Phospholipase C/protein kinase C signaling pathway-dependent phosphorylation of p44/42 and JNK MAP kinases participates partially in IL-1beta induction by TAT PubMed
tat Protein kinase C is required for HIV-1 Tat-mediated transactivation of the viral LTR promoter, indicating protein kinase C regulates the process of HIV-1 transactivation and may play a role in the transition of HIV from latency to productive growth PubMed
tat Protein kinase C phosphorylates HIV-1 Tat on serine residue 46 PubMed
tat HIV-1 Tat activates protein kinase C, resulting in the induction of TNF-alpha, IL-6 and IL-10 expression and the secretion of MCP-1 PubMed
Vpr vpr The phosphorylation of HIV-1 Gag at Ser487 by PRKCI regulates HIV-1 Vpr incorporation into virions and HIV-1 replication in macrophages PubMed
matrix gag Protein kinase C (PKC) phosphorylates HIV-1 Matrix on serine residue 111 resulting in a shift in localization of Matrix from the cytosol to the cellular membrane, suggesting a myristoyl-protein switch regulated by PKC phosphorylation PubMed
retropepsin gag-pol Phosphorylation of human recombinant vimentin by PKC inhibits the proteolytic processing of the vimentin head domain by HIV-1 protease PubMed
reverse transcriptase gag-pol HIV-1 RT heterodimer expressed in bacteria can be phosphorylated in vitro by several purified mammalian protein kinases including auto-activated protein kinase (PK), CKII, cytosolic protamine kinase (CPK), myelin basic protein kinase 1 (MBPK1), and PRKC PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • MGC26534

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables ATP binding TAS
Traceable Author Statement
more info
PubMed 
enables calcium-dependent protein kinase C activity IEA
Inferred from Electronic Annotation
more info
 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
 
enables phospholipid binding IDA
Inferred from Direct Assay
more info
PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables protein kinase C activity ISS
Inferred from Sequence or Structural Similarity
more info
 
enables protein kinase C activity TAS
Traceable Author Statement
more info
 
enables protein kinase activity IDA
Inferred from Direct Assay
more info
PubMed 
enables protein serine kinase activity IEA
Inferred from Electronic Annotation
more info
 
enables protein serine/threonine kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
enables protein serine/threonine kinase activity IDA
Inferred from Direct Assay
more info
PubMed 
Process Evidence Code Pubs
involved_in Golgi vesicle budding IEA
Inferred from Electronic Annotation
more info
 
involved_in actin filament organization IEA
Inferred from Electronic Annotation
more info
 
involved_in cell migration IEA
Inferred from Electronic Annotation
more info
 
involved_in cell-cell junction organization IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in cellular response to insulin stimulus ISS
Inferred from Sequence or Structural Similarity
more info
 
involved_in cytoskeleton organization NAS
Non-traceable Author Statement
more info
PubMed 
involved_in establishment of apical/basal cell polarity IEA
Inferred from Electronic Annotation
more info
 
involved_in establishment or maintenance of epithelial cell apical/basal polarity IDA
Inferred from Direct Assay
more info
PubMed 
involved_in establishment or maintenance of epithelial cell apical/basal polarity TAS
Traceable Author Statement
more info
PubMed 
involved_in eye photoreceptor cell development IEA
Inferred from Electronic Annotation
more info
 
involved_in intracellular signal transduction IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
involved_in membrane organization NAS
Non-traceable Author Statement
more info
PubMed 
involved_in negative regulation of apoptotic process TAS
Traceable Author Statement
more info
 
involved_in negative regulation of glial cell apoptotic process IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in negative regulation of neuron apoptotic process IDA
Inferred from Direct Assay
more info
PubMed 
involved_in peptidyl-serine phosphorylation IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
involved_in peptidyl-serine phosphorylation IDA
Inferred from Direct Assay
more info
PubMed 
involved_in positive regulation of NF-kappaB transcription factor activity IDA
Inferred from Direct Assay
more info
PubMed 
involved_in positive regulation of Notch signaling pathway TAS
Traceable Author Statement
more info
 
involved_in positive regulation of endothelial cell apoptotic process IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in positive regulation of glial cell proliferation IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in positive regulation of glucose import ISS
Inferred from Sequence or Structural Similarity
more info
 
involved_in positive regulation of neuron projection development IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in positive regulation of protein localization to plasma membrane ISS
Inferred from Sequence or Structural Similarity
more info
 
involved_in protein phosphorylation IDA
Inferred from Direct Assay
more info
PubMed 
involved_in protein targeting to membrane NAS
Non-traceable Author Statement
more info
PubMed 
involved_in regulation of postsynaptic membrane neurotransmitter receptor levels IEA
Inferred from Electronic Annotation
more info
 
involved_in response to interleukin-1 IEA
Inferred from Electronic Annotation
more info
 
involved_in secretion NAS
Non-traceable Author Statement
more info
PubMed 
involved_in vesicle-mediated transport TAS
Traceable Author Statement
more info
PubMed 
Component Evidence Code Pubs
located_in Golgi membrane IEA
Inferred from Electronic Annotation
more info
 
part_of PAR polarity complex IPI
Inferred from Physical Interaction
more info
PubMed 
located_in Schaffer collateral - CA1 synapse IEA
Inferred from Electronic Annotation
more info
 
located_in Schmidt-Lanterman incisure IEA
Inferred from Electronic Annotation
more info
 
located_in apical plasma membrane IEA
Inferred from Electronic Annotation
more info
 
located_in bicellular tight junction IEA
Inferred from Electronic Annotation
more info
 
located_in cell leading edge IEA
Inferred from Electronic Annotation
more info
 
located_in cytosol IDA
Inferred from Direct Assay
more info
PubMed 
located_in cytosol TAS
Traceable Author Statement
more info
 
located_in endosome IEA
Inferred from Electronic Annotation
more info
 
located_in extracellular exosome HDA PubMed 
located_in glutamatergic synapse IEA
Inferred from Electronic Annotation
more info
 
located_in intercellular bridge IDA
Inferred from Direct Assay
more info
 
located_in microtubule cytoskeleton IDA
Inferred from Direct Assay
more info
 
located_in nucleoplasm TAS
Traceable Author Statement
more info
 
located_in nucleus IDA
Inferred from Direct Assay
more info
PubMed 
located_in plasma membrane TAS
Traceable Author Statement
more info
 
located_in tight junction IC
Inferred by Curator
more info
PubMed 

General protein information

Preferred Names
protein kinase C iota type
Names
PRKC-lambda/iota
aPKC-lambda/iota
atypical protein kinase C-lambda/iota
NP_002731.4
XP_047304530.1
XP_047304531.1

NCBI Reference Sequences (RefSeq)

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_002740.6NP_002731.4  protein kinase C iota type

    See identical proteins and their annotated locations for NP_002731.4

    Status: REVIEWED

    Source sequence(s)
    AC023891, AC073288, BC022016, CN412350, L33881
    Consensus CDS
    CCDS3212.2
    UniProtKB/Swiss-Prot
    P41743, Q8WW06
    Related
    ENSP00000295797.4, ENST00000295797.5
    Conserved Domains (3) summary
    cd06404
    Location:26108
    PB1_aPKC; PB1 domain is an essential modular domain of the atypical protein kinase C (aPKC) which in complex with Par6 and Par3 proteins is crucial for establishment of apical-basal polarity of animal cells. PB1 domain is a modular domain mediating specific ...
    cd05618
    Location:233596
    STKc_aPKC_iota; Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C iota
    pfam00130
    Location:141191
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 110 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000003.12 Reference GRCh38.p14 Primary Assembly

    Range
    170222424..170305977
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_047448574.1XP_047304530.1  protein kinase C iota type isoform X1

  2. XM_047448575.1XP_047304531.1  protein kinase C iota type isoform X2

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060927.1 Alternate T2T-CHM13v2.0

    Range
    173007078..173090623
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)