NEIL3 nei like DNA glycosylase 3 [Homo sapiens (human)] - Gene

Summary

Official Symbol: NEIL3provided by HGNC
Official Full Name: nei like DNA glycosylase 3provided by HGNC
Primary source: HGNC:HGNC:24573
See related: Ensembl:ENSG00000109674 MIM:608934; AllianceGenome:HGNC:24573
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: FGP2; FPG2; NEI3; ZGRF3; hFPG2; hNEI3
Summary: NEIL3 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]).[supplied by OMIM, Mar 2008]
Expression: Biased expression in bone marrow (RPKM 2.5), lymph node (RPKM 1.9) and 13 other tissues See more
Orthologs: all
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Genomic context

Location:: 4q34.3

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2025_08	current	GRCh38.p14 (GCF_000001405.40)	4	NC_000004.12 (177309874..177371268)
RS_2025_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	4	NC_060928.1 (180648665..180701747)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	4	NC_000004.11 (178231028..178284090)

Chromosome 4 - NC_000004.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Tissue-specific circular RNA induction during human fetal development
Description: 35 human fetal samples from 6 tissues (3 - 7 replicates per tissue) collected between 10 and 20 weeks gestational time were sequenced using Illumina TruSeq Stranded Total RNA
BioProject: PRJNA270632
Publication: PMID 26076956
Analysis date: Mon Apr 2 22:54:59 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

NEIL3 promotes cell proliferation of ccRCC via the cyclin D1-Rb-E2F1 feedback loop regulation.
Title: NEIL3 promotes cell proliferation of ccRCC via the cyclin D1-Rb-E2F1 feedback loop regulation.
[Overexpression of Nei endonuclease VIII-like protein 3 in hepatocellular carcinoma indicates increased levels of immune infiltration and an unfavorable prognosis].
Title: [Overexpression of Nei endonuclease VIII-like protein 3 in hepatocellular carcinoma indicates increased levels of immune infiltration and an unfavorable prognosis].
EWSR1-induced circNEIL3 promotes glioma progression and exosome-mediated macrophage immunosuppressive polarization via stabilizing IGF2BP3.
Title: EWSR1-induced circNEIL3 promotes glioma progression and exosome-mediated macrophage immunosuppressive polarization via stabilizing IGF2BP3.
CircNEIL3 regulatory loop promotes pancreatic ductal adenocarcinoma progression via miRNA sponging and A-to-I RNA-editing.
Title: CircNEIL3 regulatory loop promotes pancreatic ductal adenocarcinoma progression via miRNA sponging and A-to-I RNA-editing.
DNA glycosylase Neil3 regulates vascular smooth muscle cell biology during atherosclerosis development.
Title: DNA glycosylase Neil3 regulates vascular smooth muscle cell biology during atherosclerosis development.
Deficiency of NEIL3 Enhances the Chemotherapy Resistance of Prostate Cancer.
Title: Deficiency of NEIL3 Enhances the Chemotherapy Resistance of Prostate Cancer.
An autoinhibitory role for the GRF zinc finger domain of DNA glycosylase NEIL3.
Title: An autoinhibitory role for the GRF zinc finger domain of DNA glycosylase NEIL3.
TRAIP is important for the recruitment of NEIL3 but not FANCD2, and knockdown of TRAIP promotes FA/BRCA pathway activation. Interestingly, TRAIP is non-epistatic with both NEIL3 and FA pathways in psoralen-ICL repair, suggesting that TRAIP may function upstream of the two pathways.
Title: Cooperation of the NEIL3 and Fanconi anemia/BRCA pathways in interstrand crosslink repair.
Results indicate that whilst specificity for 5-hydroxyuracil and thymine glycol was observed, NEIL3 preferentially excises oxidized bases from single stranded DNA and within open fork structures.
Title: The Biochemical Role of the Human NEIL1 and NEIL3 DNA Glycosylases on Model DNA Replication Forks.
NEIL3 cleaves psoralen-induced DNA-DNA cross-links in three-stranded and four-stranded DNA substrates to generate unhooked DNA fragments containing either an abasic site or a psoralen-thymine monoadduct. Nei and NEIL1 also cleave a psoralen-induced four-stranded DNA substrate to generate 2 unhooked DNA duplexes with a nick; NEIL3 targets both DNA strands in interstrand cross-links without generating single-strand breaks.
Title: The Human DNA glycosylases NEIL1 and NEIL3 Excise Psoralen-Induced DNA-DNA Cross-Links in a Four-Stranded DNA Structure.

Submit: New GeneRIF Correction See all GeneRIFs (30)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Genome-wide association study of electrocardiographic and heart rate variability traits: the Framingham Heart Study. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

Interaction	Pubs
Knockdown of nei endonuclease VIII-like 3 (NEIL3) by siRNA inhibits HIV-1 replication in HeLa P4/R5 cells	PubMed

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D8S2279 (e-PCR)
- UniSTS:473907

D16S325 (e-PCR)
- UniSTS:147089

SHGC-84154 (e-PCR)
- UniSTS:104673

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables DNA N-glycosylase activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA N-glycosylase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA N-glycosylase activity	IEA Inferred from Electronic Annotation more info
enables DNA N-glycosylase activity	ISS Inferred from Sequence or Structural Similarity more info
enables DNA N-glycosylase activity	TAS Traceable Author Statement more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-(apurinic or apyrimidinic site) endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-(apurinic or apyrimidinic site) endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-(apurinic or apyrimidinic site) endonuclease activity	IEA Inferred from Electronic Annotation more info
enables MCM complex binding	ISS Inferred from Sequence or Structural Similarity more info
enables bubble DNA binding	IEA Inferred from Electronic Annotation more info
enables bubble DNA binding	ISS Inferred from Sequence or Structural Similarity more info
enables catalytic activity	IEA Inferred from Electronic Annotation more info
enables class I DNA-(apurinic or apyrimidinic site) endonuclease activity	IEA Inferred from Electronic Annotation more info
enables damaged DNA binding	IEA Inferred from Electronic Annotation more info
enables damaged DNA binding	ISS Inferred from Sequence or Structural Similarity more info
enables double-stranded DNA binding	IEA Inferred from Electronic Annotation more info
enables double-stranded DNA binding	ISS Inferred from Sequence or Structural Similarity more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity, acting on glycosyl bonds	IEA Inferred from Electronic Annotation more info
enables hydrolase activity, hydrolyzing N-glycosyl compounds	IEA Inferred from Electronic Annotation more info
enables lyase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded DNA binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA damage response	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in base-excision repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in base-excision repair	IDA Inferred from Direct Assay more info	PubMed
involved_in base-excision repair	IEA Inferred from Electronic Annotation more info
involved_in base-excision repair	ISS Inferred from Sequence or Structural Similarity more info
involved_in base-excision repair, AP site formation	TAS Traceable Author Statement more info
involved_in depurination	TAS Traceable Author Statement more info
involved_in interstrand cross-link repair	ISS Inferred from Sequence or Structural Similarity more info
involved_in single strand break repair	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	IDA Inferred from Direct Assay more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	TAS Traceable Author Statement more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: endonuclease 8-like 3

Names: DNA glycosylase FPG2; DNA glycosylase hFPG2; DNA glycosylase/AP lyase Neil3; endonuclease VIII-like 3; nei endonuclease VIII-like 3; nei-like protein 3; zinc finger, GRF-type containing 3

NP_060718.3

EC 4.2.99.18

XP_047271850.1

EC 4.2.99.18

XP_054206362.1

EC 4.2.99.18

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_018248.3 → NP_060718.3 endonuclease 8-like 3

Status: VALIDATED

Source sequence(s)

AC027627

Consensus CDS

CCDS3828.1

UniProtKB/Swiss-Prot

Q2PPJ3, Q8NG51, Q8TAT5, Q9NV95

Related

ENSP00000264596.3, ENST00000264596.4

Conserved Domains (5) summary

cd08969
Location:1 → 151

MeNeil3_N; N-terminal domain of metazoan Nei-like glycosylase 3 (NEIL3)

sd00029
Location:321 → 340

zf-RanBP2; RanBP2-type Zn finger [structural motif]

pfam00641
Location:317 → 345

zf-RanBP; Zn-finger in Ran binding protein and others

pfam06839
Location:552 → 596

zf-GRF; GRF zinc finger

cl26942
Location:61 → 281

H2TH; Formamidopyrimidine-DNA glycosylase H2TH domain