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POU6F1 POU class 6 homeobox 1 [ Homo sapiens (human) ]

Gene ID: 5463, updated on 3-Mar-2026
Official Symbol
POU6F1provided by HGNC
Official Full Name
POU class 6 homeobox 1provided by HGNC
Primary source
HGNC:HGNC:9224
See related
Ensembl:ENSG00000184271 MIM:618043; AllianceGenome:HGNC:9224
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
BRN5; MPOU; TCFB1
Summary
Enables DNA-binding transcription repressor activity, RNA polymerase II-specific and sequence-specific double-stranded DNA binding activity. Involved in negative regulation of transcription by RNA polymerase II. Located in actin cytoskeleton and nuclear body. [provided by Alliance of Genome Resources, Jul 2025]
Expression
Ubiquitous expression in brain (RPKM 4.7), ovary (RPKM 3.6) and 23 other tissues See more
Orthologs
all
Try the new Gene page
Try the new Transcripts and proteins table
See POU6F1 in Genome Data Viewer
Location:
12q13.13
Exon count:
14
Annotation release Status Assembly Chr Location
RS_2025_08 current GRCh38.p14 (GCF_000001405.40) 12 NC_000012.12 (51186936..51218062, complement)
RS_2025_08 current T2T-CHM13v2.0 (GCF_009914755.1) 12 NC_060936.1 (51149818..51180972, complement)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 12 NC_000012.11 (51580719..51611846, complement)

Chromosome 12 - NC_000012.12Genomic Context describing neighboring genes Neighboring gene transcription factor CP2 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 6370 Neighboring gene RNA, U6 small nuclear 199, pseudogene Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr12:51566351-51567098 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 6371 Neighboring gene uncharacterized LOC124902933 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 4459 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 6372 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr12:51593597-51594096 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 4460 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 4461 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 4462 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 6373 Neighboring gene ReSE screen-validated silencer GRCh37_chr12:51614127-51614306 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr12:51631660-51632297 Neighboring gene Sharpr-MPRA regulatory region 9853 Neighboring gene H3K27ac hESC enhancer GRCh37_chr12:51633573-51634209 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr12:51641233-51641734 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr12:51641735-51642234 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr12:51642235-51642736 Neighboring gene DAZ associated protein 2 Neighboring gene small cell adhesion glycoprotein Neighboring gene H3K27ac hESC enhancer GRCh37_chr12:51663873-51664373 Neighboring gene bridging integrator 2 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 6375 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 6376 Neighboring gene Sharpr-MPRA regulatory region 8177 Neighboring gene uncharacterized LOC105369767 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 6377 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 4466

Go to nucleotide: Graphics FASTA GenBank

  • Project title: Tissue-specific circular RNA induction during human fetal development
  • Description: 35 human fetal samples from 6 tissues (3 - 7 replicates per tissue) collected between 10 and 20 weeks gestational time were sequenced using Illumina TruSeq Stranded Total RNA
  • BioProject: PRJNA270632
  • Publication: PMID 26076956
  • Analysis date: Mon Apr 2 22:54:59 2018

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. POU6F1 promotes ferroptosis by increasing lncRNA-CASC2 transcription to regulate SOCS2/SLC7A11 signaling in gastric cancer.
    Title: POU6F1 promotes ferroptosis by increasing lncRNA-CASC2 transcription to regulate SOCS2/SLC7A11 signaling in gastric cancer.
  2. results signify mPOU as a negative regulator of skeletal muscle differentiation and strengthen the importance of AurkA in skeletal myogenesis
    Title: Aurora kinase A-mediated phosphorylation of mPOU at a specific site drives skeletal muscle differentiation.
  3. POU6F1 siRNA dose-dependently suppressed the proliferation of clear cell adenocarcinoma cell lines.
    Title: Transcription factor POU6F1 is important for proliferation of clear cell adenocarcinoma of the ovary and is a potential new molecular target.
  4. is the transcription factor that might be involved in cell proliferation of clear cell adenocarcinoma of the ovary.
    Title: POU6F1 is the transcription factor that might be involved in cell proliferation of clear cell adenocarcinoma of the ovary.
  5. The crystal structure of human Brn-5 transcription factor in complex with corticotropin-releasing hormone gene promoter reveals an unexpected recognition mode of the protein to its cognate DNA
    Title: Structure of human Brn-5 transcription factor in complex with CRH gene promoter.
  6. Crystallization and preliminary X-ray analysis
    Title: Crystallization and preliminary X-ray analysis of human Brn-5 transcription factor in complex with DNA.
Submit: New GeneRIF Correction

Review eQTL and phenotype association data in this region using PheGenI

See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

Replication interactions

Interaction Pubs
Knockdown of POU class 6 homeobox 1 (POU6F1) by siRNA inhibits HIV-1 replication in HeLa P4/R5 cells PubMed

Go to the HIV-1, Human Interaction Database

Products Interactant Other Gene Complex Source Pubs Description

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA-binding transcription factor activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA-binding transcription factor activity TAS
Traceable Author Statement
more info
 PubMed 
enables DNA-binding transcription factor activity, RNA polymerase II-specific IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA-binding transcription factor activity, RNA polymerase II-specific ISA
Inferred from Sequence Alignment
more info
  
enables DNA-binding transcription repressor activity, RNA polymerase II-specific IDA
Inferred from Direct Assay
more info
 PubMed 
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables sequence-specific DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables sequence-specific double-stranded DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in brain development TAS
Traceable Author Statement
more info
 PubMed 
involved_in central nervous system development IEA
Inferred from Electronic Annotation
more info
  
involved_in heart development TAS
Traceable Author Statement
more info
 PubMed 
involved_in muscle organ development TAS
Traceable Author Statement
more info
 PubMed 
involved_in negative regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of transcription by RNA polymerase II IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
part_of RNA polymerase II transcription regulator complex IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in chromatin ISA
Inferred from Sequence Alignment
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
POU domain, class 6, transcription factor 1
Names
brain-5
brain-specific homeobox/POU domain protein 5
mPOU homeobox protein

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001330422.2 → NP_001317351.1  POU domain, class 6, transcription factor 1 isoform 2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) represents the longest transcript and encodes the longer isoform (2).
    Source sequence(s)
    AC139768
    Consensus CDS
    CCDS81691.1
    UniProtKB/Swiss-Prot
    A0A1C7CYV8, Q14863, Q15944, Q6DK47, Q7Z7P6
    UniProtKB/TrEMBL
    A0A1C7CYY7
    Related
    ENSP00000330190.11, ENST00000333640.12

  2. NM_001351088.2 → NP_001338017.1  POU domain, class 6, transcription factor 1 isoform 3

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4), as well as variant 12, encodes isoform 3.
    Source sequence(s)
    AC139768

  3. NM_001368008.1 → NP_001354937.1  POU domain, class 6, transcription factor 1 isoform 3

    Status: VALIDATED

    Description
    Transcript Variant: This variant (12), as well as variant 4, encodes isoform 3.
    Source sequence(s)
    AC139768

RNA

  1. NR_026893.3 RNA Sequence

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) uses an alternate splice site in an internal exon, compared to variant 3. This variant is represented as non-coding because the use of the 5'-most expected translational start codon renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
    Source sequence(s)
    AC139768
    Related
    ENST00000636728.1

  2. NR_147046.2 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC139768

  3. NR_147047.2 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC139768

  4. NR_147048.2 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC139768

  5. NR_147049.2 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC139768

  6. NR_147050.2 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC139768

  7. NR_147051.2 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC139768

  8. NR_147052.2 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC139768

  9. NR_160429.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC139768

  10. NR_160430.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC139768

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2025_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000012.12 Reference GRCh38.p14 Primary Assembly

    Range
    51186936..51218062 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_047429037.1 → XP_047284993.1  POU domain, class 6, transcription factor 1 isoform X3

    UniProtKB/TrEMBL
    A0A1C7CYY7

  2. XM_047429034.1 → XP_047284990.1  POU domain, class 6, transcription factor 1 isoform X1

    UniProtKB/Swiss-Prot
    A0A1C7CYV8, Q14863, Q15944, Q6DK47, Q7Z7P6
    UniProtKB/TrEMBL
    A0A1C7CYY7

  3. XM_047429040.1 → XP_047284996.1  POU domain, class 6, transcription factor 1 isoform X6

  4. XM_047429041.1 → XP_047284997.1  POU domain, class 6, transcription factor 1 isoform X7

  5. XM_047429039.1 → XP_047284995.1  POU domain, class 6, transcription factor 1 isoform X5

  6. XM_047429038.1 → XP_047284994.1  POU domain, class 6, transcription factor 1 isoform X4

    UniProtKB/TrEMBL
    A0A1C7CYY7

  7. XM_047429036.1 → XP_047284992.1  POU domain, class 6, transcription factor 1 isoform X2

    UniProtKB/TrEMBL
    A0A1C7CYY7

  8. XM_047429033.1 → XP_047284989.1  POU domain, class 6, transcription factor 1 isoform X1

    UniProtKB/Swiss-Prot
    A0A1C7CYV8, Q14863, Q15944, Q6DK47, Q7Z7P6
    UniProtKB/TrEMBL
    A0A1C7CYY7

  9. XM_017019514.1 → XP_016875003.1  POU domain, class 6, transcription factor 1 isoform X1

    UniProtKB/Swiss-Prot
    A0A1C7CYV8, Q14863, Q15944, Q6DK47, Q7Z7P6
    UniProtKB/TrEMBL
    A0A1C7CYY7

  10. XM_047429035.1 → XP_047284991.1  POU domain, class 6, transcription factor 1 isoform X1

    UniProtKB/Swiss-Prot
    A0A1C7CYV8, Q14863, Q15944, Q6DK47, Q7Z7P6
    UniProtKB/TrEMBL
    A0A1C7CYY7

  11. XM_047429043.1 → XP_047284999.1  POU domain, class 6, transcription factor 1 isoform X9

    UniProtKB/TrEMBL
    B3KT91

  12. XM_047429042.1 → XP_047284998.1  POU domain, class 6, transcription factor 1 isoform X8

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060936.1 Alternate T2T-CHM13v2.0

    Range
    51149818..51180972 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054372356.1 → XP_054228331.1  POU domain, class 6, transcription factor 1 isoform X3

    UniProtKB/TrEMBL
    A0A1C7CYY7

  2. XM_054372353.1 → XP_054228328.1  POU domain, class 6, transcription factor 1 isoform X1

    UniProtKB/TrEMBL
    A0A1C7CYY7

  3. XM_054372360.1 → XP_054228335.1  POU domain, class 6, transcription factor 1 isoform X7

  4. XM_054372359.1 → XP_054228334.1  POU domain, class 6, transcription factor 1 isoform X6

  5. XM_054372358.1 → XP_054228333.1  POU domain, class 6, transcription factor 1 isoform X5

  6. XM_054372355.1 → XP_054228330.1  POU domain, class 6, transcription factor 1 isoform X2

    UniProtKB/TrEMBL
    A0A1C7CYY7

  7. XM_054372357.1 → XP_054228332.1  POU domain, class 6, transcription factor 1 isoform X4

    UniProtKB/TrEMBL
    A0A1C7CYY7

  8. XM_054372352.1 → XP_054228327.1  POU domain, class 6, transcription factor 1 isoform X1

    UniProtKB/TrEMBL
    A0A1C7CYY7

  9. XM_054372351.1 → XP_054228326.1  POU domain, class 6, transcription factor 1 isoform X1

    UniProtKB/TrEMBL
    A0A1C7CYY7

  10. XM_054372354.1 → XP_054228329.1  POU domain, class 6, transcription factor 1 isoform X1

    UniProtKB/TrEMBL
    A0A1C7CYY7

  11. XM_054372350.1 → XP_054228325.1  POU domain, class 6, transcription factor 1 isoform X1

    UniProtKB/TrEMBL
    A0A1C7CYY7

  12. XM_054372362.1 → XP_054228337.1  POU domain, class 6, transcription factor 1 isoform X9

    UniProtKB/TrEMBL
    B3KT91

  13. XM_054372361.1 → XP_054228336.1  POU domain, class 6, transcription factor 1 isoform X8

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NM_002702.3: Suppressed sequence

    Description
    NM_002702.3: This RefSeq was removed because currently there is insufficient support for the transcript and the protein.
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q14863.2 GenPept UniProtKB/Swiss-Prot:Q14863

Gene LinkOut

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