Format

Send to:

Choose Destination

ACLY ATP citrate lyase [ Homo sapiens (human) ]

Gene ID: 47, updated on 9-Dec-2018

Summary

Official Symbol
ACLYprovided by HGNC
Official Full Name
ATP citrate lyaseprovided by HGNC
Primary source
HGNC:HGNC:115
See related
Ensembl:ENSG00000131473 MIM:108728
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
ACL; ATPCL; CLATP
Summary
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014]
Expression
Ubiquitous expression in prostate (RPKM 37.3), kidney (RPKM 29.2) and 25 other tissues See more
Orthologs

Genomic context

See ACLY in Genome Data Viewer
Location:
17q21.2
Exon count:
30
Annotation release Status Assembly Chr Location
109 current GRCh38.p12 (GCF_000001405.38) 17 NC_000017.11 (41866916..41930542, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 17 NC_000017.10 (40023169..40086795, complement)

Chromosome 17 - NC_000017.11Genomic Context describing neighboring genes Neighboring gene 5'-nucleotidase, cytosolic IIIB Neighboring gene kelch like family member 10 Neighboring gene kelch like family member 11 Neighboring gene tetratricopeptide repeat domain 25 Neighboring gene 2',3'-cyclic nucleotide 3' phosphodiesterase Neighboring gene DnaJ heat shock protein family (Hsp40) member C7

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Jun 15 11:32:44 2016

Bibliography

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env Tandem affinity purification and mass spectrometry analysis identify ATP citrate lyase (ACLY), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Gag-Pol gag-pol Tandem affinity purification and mass spectrometry analysis identify ATP citrate lyase (ACLY), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Nef nef Tandem affinity purification and mass spectrometry analysis identify ATP citrate lyase (ACLY), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Pr55(Gag) gag Tandem affinity purification and mass spectrometry analysis identify ATP citrate lyase (ACLY), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed

Go to the HIV-1, Human Interaction Database

Pathways from BioSystems

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • FLJ25350, FLJ44061

Gene Ontology Provided by GOA

Function Evidence Code Pubs
ATP binding TAS
Traceable Author Statement
more info
PubMed 
ATP citrate synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
ATP citrate synthase activity IDA
Inferred from Direct Assay
more info
PubMed 
ATP citrate synthase activity TAS
Traceable Author Statement
more info
PubMed 
cofactor binding IEA
Inferred from Electronic Annotation
more info
 
metal ion binding IEA
Inferred from Electronic Annotation
more info
 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
Process Evidence Code Pubs
acetyl-CoA biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
acetyl-CoA biosynthetic process IDA
Inferred from Direct Assay
more info
PubMed 
cholesterol biosynthetic process TAS
Traceable Author Statement
more info
PubMed 
citrate metabolic process IDA
Inferred from Direct Assay
more info
PubMed 
coenzyme A metabolic process TAS
Traceable Author Statement
more info
PubMed 
fatty acid biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
fatty-acyl-CoA biosynthetic process TAS
Traceable Author Statement
more info
 
lipid biosynthetic process IDA
Inferred from Direct Assay
more info
PubMed 
neutrophil degranulation TAS
Traceable Author Statement
more info
 
oxaloacetate metabolic process IDA
Inferred from Direct Assay
more info
PubMed 
positive regulation of cellular metabolic process TAS
Traceable Author Statement
more info
 
Component Evidence Code Pubs
azurophil granule lumen TAS
Traceable Author Statement
more info
 
citrate lyase complex IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
cytosol IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
cytosol IDA
Inferred from Direct Assay
more info
 
cytosol TAS
Traceable Author Statement
more info
 
extracellular exosome HDA PubMed 
extracellular region TAS
Traceable Author Statement
more info
 
ficolin-1-rich granule lumen TAS
Traceable Author Statement
more info
 
membrane HDA PubMed 
nucleoplasm IDA
Inferred from Direct Assay
more info
 
plasma membrane IDA
Inferred from Direct Assay
more info
 

General protein information

Preferred Names
ATP-citrate synthase
Names
ATP-citrate (pro-S-)-lyase
citrate cleavage enzyme
NP_001087.2
NP_001290203.1
NP_001290204.1
NP_942127.1
XP_005257452.1
XP_016880177.1

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_047031.1 RefSeqGene

    Range
    16521..68626
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001096.3NP_001087.2  ATP-citrate synthase isoform 1

    See identical proteins and their annotated locations for NP_001087.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) has an alternate splice site, which results in translation initiation at a downstream AUG start codon, compared to variant 3. The resulting isoform (1) is shorter at the N-terminus, compared to isoform 3.
    Source sequence(s)
    AC125257, BC006195, BG037168
    Consensus CDS
    CCDS11412.1
    UniProtKB/Swiss-Prot
    P53396
    UniProtKB/TrEMBL
    A0A024R1T9
    Related
    ENSP00000253792.2, ENST00000352035.6
    Conserved Domains (2) summary
    cl25712
    Location:1419
    SucC; Succinyl-CoA synthetase, beta subunit [Energy production and conversion]
    cl26126
    Location:4911094
    Ligase_CoA; CoA-ligase
  2. NM_001303274.1NP_001290203.1  ATP-citrate synthase isoform 3

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) encodes the longest isoform (3).
    Source sequence(s)
    AB210035, AK304802, DB072009
    UniProtKB/Swiss-Prot
    P53396
    UniProtKB/TrEMBL
    Q4LE36
    Conserved Domains (7) summary
    cd06100
    Location:9101147
    CCL_ACL-C; Citryl-CoA lyase (CCL), the C-terminal portion of the single-subunit type ATP-citrate lyase (ACL) and the C-terminal portion of the large subunit of the two-subunit type ACL. CCL cleaves citryl-CoA (CiCoA) to acetyl-CoA (AcCoA) and oxaloacetate (OAA). ...
    PLN02235
    Location:55473
    PLN02235; ATP citrate (pro-S)-lyase
    PLN02522
    Location:5451148
    PLN02522; ATP citrate (pro-S)-lyase
    smart00881
    Location:546655
    CoA_binding; CoA binding domain
    pfam16114
    Location:301475
    Citrate_bind; ATP citrate lyase citrate-binding
    cl17255
    Location:60261
    ATP-grasp_4; ATP-grasp domain
    cl22543
    Location:714839
    Ligase_CoA; CoA-ligase
  3. NM_001303275.1NP_001290204.1  ATP-citrate synthase isoform 4

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) lacks an in-frame coding exon, compared to variant 3. The resulting isoform (4) lacks an internal segment, compared to isoform 3.
    Source sequence(s)
    AB210035, AK295675, BC006195
    UniProtKB/Swiss-Prot
    P53396
    UniProtKB/TrEMBL
    Q4LE36
    Conserved Domains (7) summary
    cd06100
    Location:9001137
    CCL_ACL-C; Citryl-CoA lyase (CCL), the C-terminal portion of the single-subunit type ATP-citrate lyase (ACL) and the C-terminal portion of the large subunit of the two-subunit type ACL. CCL cleaves citryl-CoA (CiCoA) to acetyl-CoA (AcCoA) and oxaloacetate (OAA). ...
    PLN02235
    Location:55473
    PLN02235; ATP citrate (pro-S)-lyase
    PLN02522
    Location:5351138
    PLN02522; ATP citrate (pro-S)-lyase
    smart00881
    Location:536645
    CoA_binding; CoA binding domain
    pfam16114
    Location:301475
    Citrate_bind; ATP citrate lyase citrate-binding
    cl17255
    Location:60261
    ATP-grasp_4; ATP-grasp domain
    cl22543
    Location:704829
    Ligase_CoA; CoA-ligase
  4. NM_198830.1NP_942127.1  ATP-citrate synthase isoform 2

    See identical proteins and their annotated locations for NP_942127.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) has an alternate splice site, which results in translation initiation at a downstream AUG start codon, and lacks an in-frame coding exon, compared to variant 3. The resulting isoform (2) is shorter at the N-terminus and lacks an internal segment, compared to isoform 3.
    Source sequence(s)
    BC006195, BG037168
    Consensus CDS
    CCDS11413.1
    UniProtKB/Swiss-Prot
    P53396
    UniProtKB/TrEMBL
    A0A024R1Y2
    Related
    ENSP00000345398.1, ENST00000353196.5
    Conserved Domains (7) summary
    cd06100
    Location:8461083
    CCL_ACL-C; Citryl-CoA lyase (CCL), the C-terminal portion of the single-subunit type ATP-citrate lyase (ACL) and the C-terminal portion of the large subunit of the two-subunit type ACL. CCL cleaves citryl-CoA (CiCoA) to acetyl-CoA (AcCoA) and oxaloacetate (OAA). ...
    PLN02235
    Location:1419
    PLN02235; ATP citrate (pro-S)-lyase
    PLN02522
    Location:4811084
    PLN02522; ATP citrate (pro-S)-lyase
    smart00881
    Location:482591
    CoA_binding; CoA binding domain
    pfam16114
    Location:247421
    Citrate_bind; ATP citrate lyase citrate-binding
    cl17255
    Location:6207
    ATP-grasp_4; ATP-grasp domain
    cl22543
    Location:650775
    Ligase_CoA; CoA-ligase

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p12 Primary Assembly

Genomic

  1. NC_000017.11 Reference GRCh38.p12 Primary Assembly

    Range
    41866916..41930542 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017024688.1XP_016880177.1  ATP-citrate synthase isoform X2

    UniProtKB/TrEMBL
    A0A024R1Y2
    Conserved Domains (7) summary
    cd06100
    Location:8461083
    CCL_ACL-C; Citryl-CoA lyase (CCL), the C-terminal portion of the single-subunit type ATP-citrate lyase (ACL) and the C-terminal portion of the large subunit of the two-subunit type ACL. CCL cleaves citryl-CoA (CiCoA) to acetyl-CoA (AcCoA) and oxaloacetate (OAA). ...
    PLN02235
    Location:1419
    PLN02235; ATP citrate (pro-S)-lyase
    PLN02522
    Location:4811084
    PLN02522; ATP citrate (pro-S)-lyase
    smart00881
    Location:482591
    CoA_binding; CoA binding domain
    pfam16114
    Location:247421
    Citrate_bind; ATP citrate lyase citrate-binding
    cl17255
    Location:6207
    ATP-grasp_4; ATP-grasp domain
    cl22543
    Location:650775
    Ligase_CoA; CoA-ligase
  2. XM_005257395.1XP_005257452.1  ATP-citrate synthase isoform X1

    See identical proteins and their annotated locations for XP_005257452.1

    UniProtKB/Swiss-Prot
    P53396
    UniProtKB/TrEMBL
    A0A024R1T9
    Conserved Domains (2) summary
    cl25712
    Location:1419
    SucC; Succinyl-CoA synthetase, beta subunit [Energy production and conversion]
    cl26126
    Location:4911094
    Ligase_CoA; CoA-ligase
Support Center