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HSPA8 heat shock protein family A (Hsp70) member 8 [ Homo sapiens (human) ]

Gene ID: 3312, updated on 12-Jul-2020

Summary

Official Symbol
HSPA8provided by HGNC
Official Full Name
heat shock protein family A (Hsp70) member 8provided by HGNC
Primary source
HGNC:HGNC:5241
See related
Ensembl:ENSG00000109971 MIM:600816
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; LAP-1; NIP71; HEL-33; HSPA10; HEL-S-72p
Summary
This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Expression
Ubiquitous expression in brain (RPKM 443.0), kidney (RPKM 352.5) and 25 other tissues See more
Orthologs

Genomic context

See HSPA8 in Genome Data Viewer
Location:
11q24.1
Exon count:
10
Annotation release Status Assembly Chr Location
109.20200522 current GRCh38.p13 (GCF_000001405.39) 11 NC_000011.10 (123057489..123062366, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 11 NC_000011.9 (122928200..122933074, complement)

Chromosome 11 - NC_000011.10Genomic Context describing neighboring genes Neighboring gene ribosomal protein S26 pseudogene 43 Neighboring gene ribosomal protein L31 pseudogene 47 Neighboring gene uncharacterized LOC101929289 Neighboring gene small nucleolar RNA, C/D box 14E Neighboring gene small nucleolar RNA, C/D box 14D Neighboring gene small nucleolar RNA, C/D box 14C Neighboring gene Sharpr-MPRA regulatory region 9139 Neighboring gene CXADR like membrane protein

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env Tandem affinity purification and mass spectrometry analysis identify heat shock 70kDa protein 8 (HSPA8, HSC70), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
env Over expression of hsp70 with a herpes viral amplicon vector protects cultured hippocampal rat neurons from gp120 neurotoxicity PubMed
env The exposure of permissive CD4+ cells to HIV-1 gp120 increases the synthesis and nuclear translocation of 70kDa heat shock protein PubMed
Gag-Pol gag-pol Tandem affinity purification and mass spectrometry analysis identify heat shock 70kDa protein 8 (HSPA8, HSC70), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Nef nef Tandem affinity purification and mass spectrometry analysis identify heat shock 70kDa protein 8 (HSPA8, HSC70), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
nef Heat shock proteins Hsp40 and Hsp70 interact with HIV-1 Nef and form a complex in cells PubMed
nef HIV-1 Nef increases the production of exosomes and co-localizes with exosomal proteins CD63, AIP/Alix, AChE, Hsc70, LAMP2, and annexin A2 in HeLa cells PubMed
Pr55(Gag) gag Tandem affinity purification and mass spectrometry analysis identify heat shock 70kDa protein 8 (HSPA8, HSC70), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
gag Hsc70 is specifically incorporated into HIV-1 virions, similar to Hsp70 which is incorporated into virions through an interaction with HIV-1 Gag PubMed
Tat tat Heat shock 70kDa protein 8 (HSPA8) is identified to interact with HIV-1 Tat mutant Nullbasic in HeLa cells by LC MS/MS PubMed
tat Hsp70 and Hsp90 and Cdc37 regulate the stabilization and folding of CDK9 as well as the assembly of an active CDK9/cyclin T1 complex responsible for P-TEFb-mediated HIV-1 Tat transactivation PubMed
Vif vif HIV-1 Vif interacts with HSPA8; predicted interaction to be relevant to gene regulation PubMed
Vpr vpr HIV-1 Vpr is required for the inhibitory effect of Hsp70 on viral gene expression and replication PubMed
vpr HIV-1 Vpr competes with Hsp70 for binding to karyopherin alpha PubMed
matrix gag Hsp70 facilitates nuclear import of HIV-1 preintegration complexes by stimulating the binding of HIV-1 Matrix to karyopherin alpha PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • MGC29929, MGC131511

Gene Ontology Provided by GOA

Function Evidence Code Pubs
ATP binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
ATP binding IDA
Inferred from Direct Assay
more info
PubMed 
ATPase activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
ATPase activity IDA
Inferred from Direct Assay
more info
PubMed 
ATPase activity NAS
Non-traceable Author Statement
more info
PubMed 
ATPase activity TAS
Traceable Author Statement
more info
 
C3HC4-type RING finger domain binding IPI
Inferred from Physical Interaction
more info
PubMed 
G protein-coupled receptor binding IPI
Inferred from Physical Interaction
more info
PubMed 
MHC class II protein complex binding HDA PubMed 
RNA binding HDA PubMed 
cadherin binding HDA PubMed 
chaperone binding IPI
Inferred from Physical Interaction
more info
PubMed 
clathrin-uncoating ATPase activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
enzyme binding IPI
Inferred from Physical Interaction
more info
PubMed 
heat shock protein binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
heat shock protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
misfolded protein binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
phosphatidylserine binding IEA
Inferred from Electronic Annotation
more info
 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein binding involved in protein folding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
protein binding, bridging IPI
Inferred from Physical Interaction
more info
PubMed 
ubiquitin protein ligase binding IPI
Inferred from Physical Interaction
more info
PubMed 
unfolded protein binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
unfolded protein binding IDA
Inferred from Direct Assay
more info
PubMed 
Process Evidence Code Pubs
ATP metabolic process IDA
Inferred from Direct Assay
more info
PubMed 
axo-dendritic transport IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
cellular response to starvation TAS
Traceable Author Statement
more info
PubMed 
cellular response to unfolded protein IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
chaperone cofactor-dependent protein refolding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
chaperone-mediated autophagy ISS
Inferred from Sequence or Structural Similarity
more info
 
chaperone-mediated autophagy TAS
Traceable Author Statement
more info
PubMed 
chaperone-mediated autophagy translocation complex disassembly IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
chaperone-mediated autophagy translocation complex disassembly ISS
Inferred from Sequence or Structural Similarity
more info
 
chaperone-mediated protein transport involved in chaperone-mediated autophagy IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
chaperone-mediated protein transport involved in chaperone-mediated autophagy NAS
Non-traceable Author Statement
more info
PubMed 
clathrin coat disassembly IEA
Inferred from Electronic Annotation
more info
 
cytokine-mediated signaling pathway TAS
Traceable Author Statement
more info
 
late endosomal microautophagy IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
mRNA splicing, via spliceosome TAS
Traceable Author Statement
more info
 
membrane organization TAS
Traceable Author Statement
more info
 
negative regulation of supramolecular fiber organization IDA
Inferred from Direct Assay
more info
PubMed 
negative regulation of transcription, DNA-templated IDA
Inferred from Direct Assay
more info
PubMed 
neurotransmitter secretion TAS
Traceable Author Statement
more info
 
neutrophil degranulation TAS
Traceable Author Statement
more info
 
positive regulation by host of viral genome replication IEA
Inferred from Electronic Annotation
more info
 
positive regulation of mRNA splicing, via spliceosome IEA
Inferred from Electronic Annotation
more info
 
protein folding NAS
Non-traceable Author Statement
more info
PubMed 
protein refolding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
protein refolding IDA
Inferred from Direct Assay
more info
PubMed 
protein targeting to lysosome involved in chaperone-mediated autophagy IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
protein targeting to lysosome involved in chaperone-mediated autophagy IMP
Inferred from Mutant Phenotype
more info
PubMed 
protein targeting to lysosome involved in chaperone-mediated autophagy TAS
Traceable Author Statement
more info
PubMed 
regulation of cell cycle IEA
Inferred from Electronic Annotation
more info
 
regulation of cellular response to heat TAS
Traceable Author Statement
more info
 
regulation of mRNA stability TAS
Traceable Author Statement
more info
 
regulation of postsynapse organization IEA
Inferred from Electronic Annotation
more info
 
regulation of protein complex assembly TAS
Traceable Author Statement
more info
PubMed 
regulation of protein complex stability ISS
Inferred from Sequence or Structural Similarity
more info
 
regulation of protein import TAS
Traceable Author Statement
more info
PubMed 
regulation of protein stability IMP
Inferred from Mutant Phenotype
more info
PubMed 
response to unfolded protein IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
response to unfolded protein NAS
Non-traceable Author Statement
more info
PubMed 
slow axonal transport IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
vesicle-mediated transport IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
viral process IEA
Inferred from Electronic Annotation
more info
 
Component Evidence Code Pubs
Prp19 complex IDA
Inferred from Direct Assay
more info
PubMed 
autophagosome IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
axon IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
blood microparticle HDA PubMed 
chaperone complex IPI
Inferred from Physical Interaction
more info
PubMed 
clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane TAS
Traceable Author Statement
more info
 
cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
cytosol IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
cytosol IDA
Inferred from Direct Assay
more info
PubMed 
cytosol TAS
Traceable Author Statement
more info
 
dendrite IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
extracellular exosome HDA PubMed 
extracellular exosome IDA
Inferred from Direct Assay
more info
PubMed 
extracellular region TAS
Traceable Author Statement
more info
 
extracellular space HDA PubMed 
ficolin-1-rich granule lumen TAS
Traceable Author Statement
more info
 
focal adhesion HDA PubMed 
glutamatergic synapse IEA
Inferred from Electronic Annotation
more info
 
glycinergic synapse IEA
Inferred from Electronic Annotation
more info
 
late endosome IEA
Inferred from Electronic Annotation
more info
 
lumenal side of lysosomal membrane TAS
Traceable Author Statement
more info
PubMed 
lysosomal lumen TAS
Traceable Author Statement
more info
PubMed 
lysosomal membrane ISS
Inferred from Sequence or Structural Similarity
more info
 
lysosome IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
melanosome IEA
Inferred from Electronic Annotation
more info
 
membrane HDA PubMed 
nucleolus IEA
Inferred from Electronic Annotation
more info
 
nucleoplasm TAS
Traceable Author Statement
more info
 
nucleus IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
nucleus IDA
Inferred from Direct Assay
more info
PubMed 
perinuclear region of cytoplasm IEA
Inferred from Electronic Annotation
more info
 
photoreceptor ribbon synapse IEA
Inferred from Electronic Annotation
more info
 
plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
plasma membrane TAS
Traceable Author Statement
more info
 
postsynaptic cytosol IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
postsynaptic specialization membrane IEA
Inferred from Electronic Annotation
more info
 
presynaptic cytosol IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
ribonucleoprotein complex IDA
Inferred from Direct Assay
more info
PubMed 
secretory granule lumen TAS
Traceable Author Statement
more info
 
spliceosomal complex IEA
Inferred from Electronic Annotation
more info
 
terminal bouton IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 

General protein information

Preferred Names
heat shock cognate 71 kDa protein
Names
LPS-associated protein 1
N-myristoyltransferase inhibitor protein 71
constitutive heat shock protein 70
epididymis luminal protein 33
epididymis secretory sperm binding protein Li 72p
heat shock 70kDa protein 8
heat shock 70kd protein 10
heat shock cognate protein 54
lipopolysaccharide-associated protein 1

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_029473.1 RefSeqGene

    Range
    5001..9648
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_006597.6NP_006588.1  heat shock cognate 71 kDa protein isoform 1

    See identical proteins and their annotated locations for NP_006588.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1).
    Source sequence(s)
    AK222628, AP000926, BC019816, DC395565
    Consensus CDS
    CCDS8440.1
    UniProtKB/Swiss-Prot
    P11142
    UniProtKB/TrEMBL
    Q53HF2, V9HW22
    Related
    ENSP00000432083.1, ENST00000534624.6
    Conserved Domains (1) summary
    PTZ00009
    Location:1613
    PTZ00009; heat shock 70 kDa protein; Provisional
  2. NM_153201.4NP_694881.1  heat shock cognate 71 kDa protein isoform 2

    See identical proteins and their annotated locations for NP_694881.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) uses alternate splice sites, and is missing an exon in the 3' coding region compared to variant 1. However, it maintains the reading frame, and encodes a shorter isoform (2) missing an internal protein segment compared to isoform 1.
    Source sequence(s)
    AB034951, AK222628, AP000926, DC395565
    Consensus CDS
    CCDS44754.1
    UniProtKB/Swiss-Prot
    P11142
    UniProtKB/TrEMBL
    Q53HF2
    Related
    ENSP00000404372.2, ENST00000453788.6
    Conserved Domains (1) summary
    cl26953
    Location:1462
    HSP70; Hsp70 protein

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000011.10 Reference GRCh38.p13 Primary Assembly

    Range
    123057489..123062366 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011542798.1XP_011541100.1  heat shock cognate 71 kDa protein isoform X1

    See identical proteins and their annotated locations for XP_011541100.1

    UniProtKB/Swiss-Prot
    P11142
    UniProtKB/TrEMBL
    V9HW22
    Related
    ENSP00000227378.3, ENST00000227378.7
    Conserved Domains (1) summary
    PTZ00009
    Location:1613
    PTZ00009; heat shock 70 kDa protein; Provisional
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