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DNAJA1 DnaJ heat shock protein family (Hsp40) member A1 [ Homo sapiens (human) ]

Gene ID: 3301, updated on 3-Mar-2019

Summary

Official Symbol
DNAJA1provided by HGNC
Official Full Name
DnaJ heat shock protein family (Hsp40) member A1provided by HGNC
Primary source
HGNC:HGNC:5229
See related
Ensembl:ENSG00000086061 MIM:602837
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
DJ-2; DjA1; HDJ2; HSDJ; HSJ2; HSJ-2; HSPF4; NEDD7; hDJ-2
Summary
This gene encodes a member of the DnaJ family of proteins, which act as heat shock protein 70 cochaperones. Heat shock proteins facilitate protein folding, trafficking, prevention of aggregation, and proteolytic degradation. Members of this family are characterized by a highly conserved N-terminal J domain, a glycine/phenylalanine-rich region, four CxxCxGxG zinc finger repeats, and a C-terminal substrate-binding domain. The J domain mediates the interaction with heat shock protein 70 to recruit substrates and regulate ATP hydrolysis activity. In humans, this gene has been implicated in positive regulation of virus replication through co-option by the influenza A virus. Several pseudogenes of this gene are found on other chromosomes. [provided by RefSeq, Sep 2015]
Expression
Ubiquitous expression in testis (RPKM 94.7), brain (RPKM 86.5) and 25 other tissues See more
Orthologs

Genomic context

See DNAJA1 in Genome Data Viewer
Location:
9p21.1
Exon count:
9
Annotation release Status Assembly Chr Location
109 current GRCh38.p12 (GCF_000001405.38) 9 NC_000009.12 (33025203..33039907)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 9 NC_000009.11 (33025209..33039905)

Chromosome 9 - NC_000009.12Genomic Context describing neighboring genes Neighboring gene aprataxin Neighboring gene transcription elongation factor A protein 1 pseudogene Neighboring gene L antigen family member 3 pseudogene 1 Neighboring gene SMU1 DNA replication regulator and spliceosomal factor Neighboring gene beta-1,4-galactosyltransferase 1 Neighboring gene B4GALT1 antisense RNA 1

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

HIV-1 interactions

Replication interactions

Interaction Pubs
Knockdown of DnaJ (Hsp40) homolog, subfamily A, member 1 (DNAJA1) by siRNA inhibits HIV-1 replication in CD4+/CCR5+/CXCR4+ TZM-bl HeLa cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Rev rev The expression of HSP40A1 (DNAJA1) inhibits HIV-1 Rev expression at the posttranscriptional levels, leading to the reduction of HIV-1 RNA accumulation PubMed
Vif vif HIV-1 Vif interacts with DNAJA1; interaction predicted to be relevant in protein folding and/or macromolecular structure assembly PubMed
capsid gag HSP40A1 (DNAJA1) inhibits HIV-1 production as measured by the level of viral p24 capsid in the culture supernatant. The HSP40A1 (1-83) mutant lacking most of the C-terminal portion is more potent in inhibiting HIV-1 production than its wild-type PubMed

Go to the HIV-1, Human Interaction Database

Pathways from BioSystems

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
ATP binding IEA
Inferred from Electronic Annotation
more info
 
ATPase activator activity IDA
Inferred from Direct Assay
more info
PubMed 
C3HC4-type RING finger domain binding IPI
Inferred from Physical Interaction
more info
PubMed 
G protein-coupled receptor binding IPI
Inferred from Physical Interaction
more info
PubMed 
Hsp70 protein binding IDA
Inferred from Direct Assay
more info
PubMed 
Tat protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
chaperone binding IDA
Inferred from Direct Assay
more info
PubMed 
chaperone binding IPI
Inferred from Physical Interaction
more info
PubMed 
low-density lipoprotein particle receptor binding IDA
Inferred from Direct Assay
more info
PubMed 
metal ion binding IEA
Inferred from Electronic Annotation
more info
 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
ubiquitin protein ligase binding IPI
Inferred from Physical Interaction
more info
PubMed 
unfolded protein binding IEA
Inferred from Electronic Annotation
more info
 
Process Evidence Code Pubs
DNA damage response, detection of DNA damage IEA
Inferred from Electronic Annotation
more info
 
androgen receptor signaling pathway IEA
Inferred from Electronic Annotation
more info
 
flagellated sperm motility IEA
Inferred from Electronic Annotation
more info
 
negative regulation of JUN kinase activity IMP
Inferred from Mutant Phenotype
more info
PubMed 
negative regulation of apoptotic process IMP
Inferred from Mutant Phenotype
more info
PubMed 
negative regulation of establishment of protein localization to mitochondrion IDA
Inferred from Direct Assay
more info
PubMed 
negative regulation of nitrosative stress-induced intrinsic apoptotic signaling pathway IDA
Inferred from Direct Assay
more info
PubMed 
negative regulation of protein ubiquitination IDA
Inferred from Direct Assay
more info
PubMed 
positive regulation of ATPase activity IEA
Inferred from Electronic Annotation
more info
 
positive regulation of apoptotic process IMP
Inferred from Mutant Phenotype
more info
PubMed 
protein folding IEA
Inferred from Electronic Annotation
more info
 
protein localization to mitochondrion IMP
Inferred from Mutant Phenotype
more info
PubMed 
regulation of protein transport IDA
Inferred from Direct Assay
more info
PubMed 
response to heat IEA
Inferred from Electronic Annotation
more info
 
response to unfolded protein TAS
Traceable Author Statement
more info
PubMed 
spermatogenesis IEA
Inferred from Electronic Annotation
more info
 
toxin transport IEA
Inferred from Electronic Annotation
more info
 
Component Evidence Code Pubs
cytoplasmic side of endoplasmic reticulum membrane TAS
Traceable Author Statement
more info
PubMed 
cytosol IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
cytosol IDA
Inferred from Direct Assay
more info
PubMed 
cytosol TAS
Traceable Author Statement
more info
 
extracellular exosome HDA PubMed 
membrane HDA PubMed 
membrane IDA
Inferred from Direct Assay
more info
PubMed 
microtubule cytoskeleton IDA
Inferred from Direct Assay
more info
 
mitochondrion IEA
Inferred from Electronic Annotation
more info
 
nucleus IEA
Inferred from Electronic Annotation
more info
 
perinuclear region of cytoplasm IEA
Inferred from Electronic Annotation
more info
 
colocalizes_with ubiquitin ligase complex IDA
Inferred from Direct Assay
more info
PubMed 

General protein information

Preferred Names
dnaJ homolog subfamily A member 1
Names
DnaJ (Hsp40) homolog, subfamily A, member 1
dnaJ protein homolog 2
heat shock 40 kDa protein 4
heat shock protein J2
heat shock protein, DNAJ-like 2
human DnaJ protein 2
neural precursor cell expressed, developmentally down-regulated 7

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001314039.1NP_001300968.1  dnaJ homolog subfamily A member 1 isoform 2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region, which results in use of a downstream start codon compared to variant 1. It encodes isoform 2, which has a shorter N-terminus than isoform 1.
    Source sequence(s)
    AK095541, AK298725, BM469197, BU508753, DA003255, DB575768
    UniProtKB/TrEMBL
    B7Z5C0
    Conserved Domains (1) summary
    cl28246
    Location:2237
    DnaJ; DnaJ-class molecular chaperone with C-terminal Zn finger domain [Posttranslational modification, protein turnover, chaperones]
  2. NM_001539.4NP_001530.1  dnaJ homolog subfamily A member 1 isoform 1

    See identical proteins and their annotated locations for NP_001530.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
    Source sequence(s)
    AK095541, BC008182, BI462555, BU508753, DB575768
    Consensus CDS
    CCDS6533.1
    UniProtKB/Swiss-Prot
    P31689
    Related
    ENSP00000369127.3, ENST00000330899.4
    Conserved Domains (1) summary
    cl28246
    Location:2394
    DnaJ; DnaJ-class molecular chaperone with C-terminal Zn finger domain [Posttranslational modification, protein turnover, chaperones]

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p12 Primary Assembly

Genomic

  1. NC_000009.12 Reference GRCh38.p12 Primary Assembly

    Range
    33025203..33039907
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
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