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MARCHF8 membrane associated ring-CH-type finger 8 [ Homo sapiens (human) ]

Gene ID: 220972, updated on 21-Mar-2023

Summary

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Official Symbol
MARCHF8provided by HGNC
Official Full Name
membrane associated ring-CH-type finger 8provided by HGNC
Primary source
HGNC:HGNC:23356
See related
Ensembl:ENSG00000165406 MIM:613335; AllianceGenome:HGNC:23356
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
MIR; CMIR; c-MIR; MARCH8; RNF178; MARCH-VIII
Summary
MARCH8 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH8 induces the internalization of several membrane glycoproteins (Goto et al., 2003 [PubMed 12582153]; Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Apr 2010]
Expression
Ubiquitous expression in testis (RPKM 18.4), bone marrow (RPKM 12.0) and 25 other tissues See more
Orthologs
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Genomic context

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See MARCHF8 in Genome Data Viewer
Location:
10q11.21-q11.22
Exon count:
15
Annotation release Status Assembly Chr Location
RS_2023_03 current GRCh38.p14 (GCF_000001405.40) 10 NC_000010.11 (45454585..45594907, complement)
RS_2023_03 current T2T-CHM13v2.0 (GCF_009914755.1) 10 NC_060934.1 (46335573..46475913, complement)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 10 NC_000010.10 (45950033..46090355, complement)

Chromosome 10 - NC_000010.11Genomic Context describing neighboring genes Neighboring gene olfactory receptor family 13 subfamily A member 1 Neighboring gene arachidonate 5-lipoxygenase Neighboring gene uncharacterized LOC102724323 Neighboring gene uncharacterized LOC105378286 Neighboring gene VISTA enhancer hs2065 Neighboring gene zinc finger AN1-type containing 4 Neighboring gene ArfGAP with GTPase domain, ankyrin repeat and PH domain 10, pseudogene

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. MARCH8 Restricts Influenza A Virus Infectivity but Does Not Downregulate Viral Glycoprotein Expression at the Surface of Infected Cells. Villalón-Letelier F, et al. mBio, 2021 Oct 26. PMID 34517760, Free PMC Article
  2. MARCH8 Inhibits Ebola Virus Glycoprotein, Human Immunodeficiency Virus Type 1 Envelope Glycoprotein, and Avian Influenza Virus H5N1 Hemagglutinin Maturation. Yu C, et al. mBio, 2020 Sep 15. PMID 32934085, Free PMC Article
  3. MARCH8 inhibits viral infection by two different mechanisms. Zhang Y, et al. Elife, 2020 Aug 11. PMID 32778221, Free PMC Article
  4. Sh-MARCH8 Inhibits Tumorigenesis via PI3K Pathway in Gastric Cancer. Yin J, et al. Cell Physiol Biochem, 2018. PMID 30138931
  5. Yersinia pestis acetyltransferase-mediated dual acetylation at the serine and lysine residues enhances the auto-ubiquitination of ubiquitin ligase MARCH8 in human cells. Li C, et al. Cell Cycle, 2017 Apr 3. PMID 28103160, Free PMC Article

See all (52) citations in PubMed

See citations in PubMed for homologs of this gene provided by HomoloGene

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Membrane-associated RING-CH protein (MARCH8) is a novel glycolysis repressor targeted by miR-32 in colorectal cancer.
    Title: Membrane-associated RING-CH protein (MARCH8) is a novel glycolysis repressor targeted by miR-32 in colorectal cancer.
  2. Membrane-Associated RING-CH 8 Functions as a Novel PD-L1 E3 Ligase to Mediate PD-L1 Degradation Induced by EGFR Inhibitors.
    Title: Membrane-Associated RING-CH 8 Functions as a Novel PD-L1 E3 Ligase to Mediate PD-L1 Degradation Induced by EGFR Inhibitors.
  3. MARCH8 Restricts Influenza A Virus Infectivity but Does Not Downregulate Viral Glycoprotein Expression at the Surface of Infected Cells.
    Title: MARCH8 Restricts Influenza A Virus Infectivity but Does Not Downregulate Viral Glycoprotein Expression at the Surface of Infected Cells.
  4. MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.
    Title: MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.
  5. MARCH8 Inhibits Ebola Virus Glycoprotein, Human Immunodeficiency Virus Type 1 Envelope Glycoprotein, and Avian Influenza Virus H5N1 Hemagglutinin Maturation.
    Title: MARCH8 Inhibits Ebola Virus Glycoprotein, Human Immunodeficiency Virus Type 1 Envelope Glycoprotein, and Avian Influenza Virus H5N1 Hemagglutinin Maturation.
  6. MARCH8 inhibits viral infection by two different mechanisms.
    Title: MARCH8 inhibits viral infection by two different mechanisms.
  7. MicroRNA-199a-5p regulates glioma progression via targeting MARCH8.
    Title: MicroRNA-199a-5p regulates glioma progression via targeting MARCH8.
  8. MHC II ubiquitination by E3 ubiquitin-protein ligase MARCH8 (MARCH8) and CD83 antigen (CD83) plays a critical role in T cell thymic selection [Review].
    Title: MARCH ligases in immunity.
  9. MARCH8, a RING-finger E3 ligase, catalyzes K63-linked NS2 polyubiquitination in vitro and in HCV-infected cells. MARCH8 is required for infection with HCV, dengue, and Zika viruses and specifically mediates HCV envelopment.
    Title: MARCH8 Ubiquitinates the Hepatitis C Virus Nonstructural 2 Protein and Mediates Viral Envelopment.
  10. Knockdown of MARCH8 inactivated the PI3K and ss-catenin stat3 signaling pathways by changing protein expression levels or the phosphorylation of related proteins and inhibited migration and invasion of MKN-45 and AGS cells.
    Title: Sh-MARCH8 Inhibits Tumorigenesis via PI3K Pathway in Gastric Cancer.
Submit: New GeneRIF Correction See all GeneRIFs (25)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
A meta-analysis of 87,040 individuals identifies 23 new susceptibility loci for prostate cancer.
EBI GWAS Catalog
An atlas of genetic influences on human blood metabolites.
EBI GWAS Catalog
Discovery and refinement of loci associated with lipid levels.
EBI GWAS Catalog
Genome-wide association study of hematological and biochemical traits in a Japanese population.
EBI GWAS Catalog
Multiple loci influence erythrocyte phenotypes in the CHARGE Consortium.
EBI GWAS Catalog
Seventy-five genetic loci influencing the human red blood cell.
EBI GWAS Catalog

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

HIV-1 interactions

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Replication interactions

Interaction Pubs
HIV-1 replication is inhibited by MARCH8 expression in monocyte derived macrophages as replication is recovered through MARCH8 knockdown by shRNA PubMed
HIV-1 replication is inhibited by MARCH8 expression in 293T or MAGIC5 cells PubMed
Knockdown of membrane-associated ring finger (C3HC4) 8, E3 ubiquitin protein ligase (MARCH8) by siRNA enhances the early stages of HIV-1 replication in HeLa-CD4 cells infected with viral pseudotypes HIV89.6R and HIV8.2N PubMed

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env HIV-1 Env (gp120) binds MARCH8 leading to Env (gp120) removal from the surface of cells PubMed
env HIV-1 Env (gp120) incorporation into virions is inhibited by MARCH8 expression PubMed
env HIV-1 Env (gp120) binds MARCH8 leading to Env (gp120) downregulation from the surface of cells PubMed
Pr55(Gag) gag Expression of MARCH-8 inhibits HLA-DR-mediated enhancement of mature Gag products internalization by downregulating cell surface HLA-DR PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables MHC protein binding IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables ubiquitin protein ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
enables ubiquitin-protein transferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in adaptive immune response IEA
Inferred from Electronic Annotation
more info
  
involved_in antigen processing and presentation of peptide antigen via MHC class II IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
involved_in immune response IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
involved_in protein polyubiquitination IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
involved_in protein polyubiquitination IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
is_active_in early endosome membrane IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
located_in endosome IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in late endosome membrane IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
located_in lysosomal membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in lysosome IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
located_in lysosome IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
E3 ubiquitin-protein ligase MARCHF8
Names
E3 ubiquitin-protein ligase MARCH8
RING finger protein 178
RING-type E3 ubiquitin transferase MARCH8
RING-type E3 ubiquitin transferase MARCHF8
c-mir, cellular modulator of immune recognition
cellular modulator of immune recognition (c-MIR)
membrane associated ring finger 8
membrane-associated RING finger protein 8
membrane-associated RING-CH protein VIII
membrane-associated ring finger (C3HC4) 8, E3 ubiquitin protein ligase
NP_001002266.1
NP_001269795.1
NP_001388574.1
NP_001388575.1
NP_659458.2
XP_011537794.1
XP_011537797.1
XP_047280719.1
XP_047280720.1
XP_047280721.1
XP_047280722.1
XP_047280723.1
XP_047280724.1
XP_047280725.1
XP_054221068.1
XP_054221069.1
XP_054221070.1
XP_054221071.1
XP_054221072.1
XP_054221073.1
XP_054221074.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001002266.3 → NP_001002266.1  E3 ubiquitin-protein ligase MARCHF8 isoform a

    See identical proteins and their annotated locations for NP_001002266.1

    Status: VALIDATED

    Source sequence(s)
    AK023636, AK025472, AL833316, BC025394, BM151615
    Consensus CDS
    CCDS7213.1
    UniProtKB/Swiss-Prot
    Q5T0T0, Q8TC72
    UniProtKB/TrEMBL
    A0A024R7U2
    Related
    ENSP00000379116.2, ENST00000395769.6
    Conserved Domains (1) summary
    smart00744
    Location:79 → 127
    RINGv; The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins

  2. NM_001282866.2 → NP_001269795.1  E3 ubiquitin-protein ligase MARCHF8 isoform b

    See identical proteins and their annotated locations for NP_001269795.1

    Status: VALIDATED

    Source sequence(s)
    AK023636, AK025472, AK307667, AL833316, BM151615
    Consensus CDS
    CCDS60519.1
    UniProtKB/Swiss-Prot
    Q5T0T0
    Related
    ENSP00000411848.2, ENST00000453424.7
    Conserved Domains (1) summary
    smart00744
    Location:361 → 409
    RINGv; The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins

  3. NM_001401645.1 → NP_001388574.1  E3 ubiquitin-protein ligase MARCHF8 isoform b

    Status: VALIDATED

    Source sequence(s)
    AL445201, AL512595, AL731567
    Consensus CDS
    CCDS60519.1

  4. NM_001401646.1 → NP_001388575.1  E3 ubiquitin-protein ligase MARCHF8 isoform b

    Status: VALIDATED

    Source sequence(s)
    AL445201, AL512595, AL731567
    Consensus CDS
    CCDS60519.1

  5. NM_145021.6 → NP_659458.2  E3 ubiquitin-protein ligase MARCHF8 isoform a

    See identical proteins and their annotated locations for NP_659458.2

    Status: VALIDATED

    Source sequence(s)
    AK023636, AK025472, AL445201, AL833316, BC066988, BM151615, HY047787
    Consensus CDS
    CCDS7213.1
    UniProtKB/Swiss-Prot
    Q5T0T0, Q8TC72
    UniProtKB/TrEMBL
    A0A024R7U2
    Related
    ENSP00000317087.3, ENST00000319836.7
    Conserved Domains (1) summary
    smart00744
    Location:79 → 127
    RINGv; The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_03

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000010.11 Reference GRCh38.p14 Primary Assembly

    Range
    45454585..45594907 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_047424768.1 → XP_047280724.1  E3 ubiquitin-protein ligase MARCHF8 isoform X2

    UniProtKB/Swiss-Prot
    Q8TC72
    UniProtKB/TrEMBL
    A0A024R7U2

  2. XM_011539492.4 → XP_011537794.1  E3 ubiquitin-protein ligase MARCHF8 isoform X1

    See identical proteins and their annotated locations for XP_011537794.1

    UniProtKB/Swiss-Prot
    Q5T0T0
    Conserved Domains (1) summary
    smart00744
    Location:361 → 409
    RINGv; The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins

  3. XM_047424765.1 → XP_047280721.1  E3 ubiquitin-protein ligase MARCHF8 isoform X1

  4. XM_047424769.1 → XP_047280725.1  E3 ubiquitin-protein ligase MARCHF8 isoform X2

    UniProtKB/Swiss-Prot
    Q8TC72
    UniProtKB/TrEMBL
    A0A024R7U2

  5. XM_047424764.1 → XP_047280720.1  E3 ubiquitin-protein ligase MARCHF8 isoform X1

  6. XM_047424766.1 → XP_047280722.1  E3 ubiquitin-protein ligase MARCHF8 isoform X1

  7. XM_047424767.1 → XP_047280723.1  E3 ubiquitin-protein ligase MARCHF8 isoform X2

    UniProtKB/Swiss-Prot
    Q8TC72
    UniProtKB/TrEMBL
    A0A024R7U2

  8. XM_047424763.1 → XP_047280719.1  E3 ubiquitin-protein ligase MARCHF8 isoform X1

  9. XM_011539495.2 → XP_011537797.1  E3 ubiquitin-protein ligase MARCHF8 isoform X2

    See identical proteins and their annotated locations for XP_011537797.1

    UniProtKB/Swiss-Prot
    Q5T0T0, Q8TC72
    UniProtKB/TrEMBL
    A0A024R7U2
    Conserved Domains (1) summary
    smart00744
    Location:79 → 127
    RINGv; The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins

RNA

  1. XR_007061947.1 RNA Sequence

Reference GRCh38.p14 ALT_REF_LOCI_1

Genomic

  1. NW_003315935.1 Reference GRCh38.p14 ALT_REF_LOCI_1

    Range
    295416..305254 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060934.1 Alternate T2T-CHM13v2.0

    Range
    46335573..46475913 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054365098.1 → XP_054221073.1  E3 ubiquitin-protein ligase MARCHF8 isoform X2

  2. XM_054365093.1 → XP_054221068.1  E3 ubiquitin-protein ligase MARCHF8 isoform X1

  3. XM_054365095.1 → XP_054221070.1  E3 ubiquitin-protein ligase MARCHF8 isoform X1

  4. XM_054365099.1 → XP_054221074.1  E3 ubiquitin-protein ligase MARCHF8 isoform X2

  5. XM_054365094.1 → XP_054221069.1  E3 ubiquitin-protein ligase MARCHF8 isoform X1

  6. XM_054365096.1 → XP_054221071.1  E3 ubiquitin-protein ligase MARCHF8 isoform X2

  7. XM_054365097.1 → XP_054221072.1  E3 ubiquitin-protein ligase MARCHF8 isoform X2

RNA

  1. XR_008488176.1 RNA Sequence

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NM_001002265.1: Suppressed sequence

    Description
    NM_001002265.1: This RefSeq was permanently suppressed because currently there is insufficient support for the transcript and the protein.
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q5T0T0.1 GenPept UniProtKB/Swiss-Prot:Q5T0T0

Gene LinkOut

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