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Plat plasminogen activator, tissue [ Mus musculus (house mouse) ]

Gene ID: 18791, updated on 22-Apr-2024

Summary

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Official Symbol
Platprovided by MGI
Official Full Name
plasminogen activator, tissueprovided by MGI
Primary source
MGI:MGI:97610
See related
Ensembl:ENSMUSG00000031538 AllianceGenome:MGI:97610
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
tPA; D8Ertd2e
Summary
This gene encodes a key enzyme of the fibrinolytic pathway. The encoded protein undergoes proteolytic processing by plasmin to generate a heterodimeric serine protease that cleaves the proenzyme plasminogen to produce plasmin, a protease that is required to break down fibrin clots. Additionally, the encoded protein is involved in other biological processes such as synaptic plasticity, cell migration and tissue remodeling. Mice lacking the encoded protein display a reduction in long-term potentiation in hippocampus and conversely, transgenic mice overexpressing the encoded protein have increased and prolonged long-term potentiation. [provided by RefSeq, Jul 2015]
Expression
Broad expression in ovary adult (RPKM 44.8), subcutaneous fat pad adult (RPKM 41.2) and 20 other tissues See more
Orthologs
human all
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Genomic context

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Location:
8 A2; 8 11.42 cM
Exon count:
15
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 8 NC_000074.7 (23247655..23272864)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 8 NC_000074.6 (22757722..22782848)

Chromosome 8 - NC_000074.7Genomic Context describing neighboring genes Neighboring gene RIKEN cDNA A930013F10 gene Neighboring gene STARR-positive B cell enhancer ABC_E6626 Neighboring gene polymerase (DNA directed), beta Neighboring gene inhibitor of kappaB kinase beta Neighboring gene predicted gene 15346 Neighboring gene STARR-seq mESC enhancer starr_20910 Neighboring gene RIKEN cDNA 1700041G16 gene Neighboring gene STARR-seq mESC enhancer starr_20911 Neighboring gene STARR-positive B cell enhancer ABC_E188 Neighboring gene STARR-positive B cell enhancer ABC_E8844 Neighboring gene STARR-positive B cell enhancer ABC_E2859 Neighboring gene STARR-positive B cell enhancer ABC_E6627 Neighboring gene STARR-positive B cell enhancer ABC_E2259 Neighboring gene adaptor-related protein complex 3, mu 2 subunit Neighboring gene STARR-seq mESC enhancer starr_20912 Neighboring gene predicted gene, 46036 Neighboring gene predicted gene, 24735

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Role of tPA in Corticosterone-Induced Apoptosis of Mouse Mural Granulosa and Oviductal Epithelial Cells. Hua Q, et al. Cells, 2023 Jan 31. PMID 36766799, Free PMC Article
  2. Blood tissue Plasminogen Activator (tPA) of liver origin contributes to neurovascular coupling involving brain endothelial N-Methyl-D-Aspartate (NMDA) receptors. Furon J, et al. Fluids Barriers CNS, 2023 Feb 3. PMID 36737775, Free PMC Article
  3. Thrombolytic tPA-induced hemorrhagic transformation of ischemic stroke is mediated by PKCβ phosphorylation of occludin. Goncalves A, et al. Blood, 2022 Jul 28. PMID 35576527, Free PMC Article
  4. Protease-independent action of tissue plasminogen activator in brain plasticity and neurological recovery after ischemic stroke. Pu H, et al. Proc Natl Acad Sci U S A, 2019 Apr 30. PMID 30996120, Free PMC Article
  5. Investigations on the Role of the Fibrinolytic Pathway on Outflow Facility Regulation. Hu Y, et al. Invest Ophthalmol Vis Sci, 2019 Apr 1. PMID 30995314, Free PMC Article

See all (341) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The Janus face of endogenous neuronal tPA: promoting self-protection and worsening the death of neighboring neurons.
    Title: The Janus face of endogenous neuronal tPA: promoting self-protection and worsening the death of neighboring neurons.
  2. Mechanical induction of osteoanabolic Wnt1 promotes osteoblast differentiation via Plat.
    Title: Mechanical induction of osteoanabolic Wnt1 promotes osteoblast differentiation via Plat.
  3. Role of tPA in Corticosterone-Induced Apoptosis of Mouse Mural Granulosa and Oviductal Epithelial Cells.
    Title: Role of tPA in Corticosterone-Induced Apoptosis of Mouse Mural Granulosa and Oviductal Epithelial Cells.
  4. Blood tissue Plasminogen Activator (tPA) of liver origin contributes to neurovascular coupling involving brain endothelial N-Methyl-D-Aspartate (NMDA) receptors.
    Title: Blood tissue Plasminogen Activator (tPA) of liver origin contributes to neurovascular coupling involving brain endothelial N-Methyl-D-Aspartate (NMDA) receptors.
  5. tPA Deficiency Underlies Neurovascular Coupling Dysfunction by Amyloid-beta.
    Title: tPA Deficiency Underlies Neurovascular Coupling Dysfunction by Amyloid-β.
  6. Interacting hepatic PAI-1/tPA gene regulatory pathways influence impaired fibrinolysis severity in obesity.
    Title: Interacting hepatic PAI-1/tPA gene regulatory pathways influence impaired fibrinolysis severity in obesity.
  7. This effect requires endothelial NMDAR and is t-PA-dependent. These neurodevelopmental data reinforce the debate regarding safety of molecules with NMDA-antagonist properties administered to preterm and term neonates.
    Title: Glutamate controls vessel-associated migration of GABA interneurons from the pial migratory route via NMDA receptors and endothelial protease activation.
  8. Sata suggest that tissue-type plasminogen activator (tPA) regulates nocturnal wheel-running activity, and that tPA differentially affects suprachiasmatic nucleus-driven nocturnal activity rhythms and activity driven by fasting or temporal food restriction.
    Title: Regulation of Locomotor activity in fed, fasted, and food-restricted mice lacking tissue-type plasminogen activator.
  9. Our findings suggest that isoflurane pretreatment could attenuate tPA-exaggerated brain ischemic injury, by reducing tPA-induced LRP/NF-kappaB/Cox-2 in endothelial cells, endothelial MMP-2 and MMP-9 activation, and subsequent pro-apoptotic molecule in neurons after oxygen/glucose deprivation and reperfusion.
    Title: Isoflurane preconditioning inhibits the effects of tissue-type plasminogen activator on brain endothelial cell in an in vitro model of ischemic stroke.
  10. Tissue plasminogen activator was found to be restricted to subsets of excitatory pyramidal glutamatergic neurons.
    Title: Tissue Plasminogen Activator Expression Is Restricted to Subsets of Excitatory Pyramidal Glutamatergic Neurons.
Submit: New GeneRIF Correction See all GeneRIFs (124)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • MGC18508

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables peptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables phosphoprotein binding ISO
Inferred from Sequence Orthology
more info
  
enables serine-type endopeptidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables serine-type endopeptidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables serine-type endopeptidase activity ISO
Inferred from Sequence Orthology
more info
  
enables serine-type peptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables signaling receptor binding ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in negative regulation of fibrinolysis NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in negative regulation of plasminogen activation NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in negative regulation of proteolysis ISO
Inferred from Sequence Orthology
more info
  
involved_in plasminogen activation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in plasminogen activation ISO
Inferred from Sequence Orthology
more info
  
involved_in platelet-derived growth factor receptor signaling pathway IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within platelet-derived growth factor receptor signaling pathway IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within platelet-derived growth factor receptor signaling pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in prevention of polyspermy ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within proteolysis IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within response to hypoxia IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in smooth muscle cell migration IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within smooth muscle cell migration IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in synaptic transmission, glutamatergic ISO
Inferred from Sequence Orthology
more info
  
involved_in trans-synaptic signaling by BDNF, modulating synaptic transmission IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in trans-synaptic signaling by BDNF, modulating synaptic transmission IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in Schaffer collateral - CA1 synapse IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in Schaffer collateral - CA1 synapse IMP
Inferred from Mutant Phenotype
more info
 PubMed 
located_in apical part of cell IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cell surface ISO
Inferred from Sequence Orthology
more info
  
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
  
located_in extracellular region IEA
Inferred from Electronic Annotation
more info
  
is_active_in extracellular space IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in extracellular space IDA
Inferred from Direct Assay
more info
 PubMed 
located_in extracellular space ISO
Inferred from Sequence Orthology
more info
  
is_active_in glutamatergic synapse IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in glutamatergic synapse IMP
Inferred from Mutant Phenotype
more info
 PubMed 
is_active_in glutamatergic synapse ISO
Inferred from Sequence Orthology
more info
  
is_active_in neuronal dense core vesicle ISO
Inferred from Sequence Orthology
more info
  
is_active_in perisynaptic space ISO
Inferred from Sequence Orthology
more info
  
is_active_in postsynapse ISO
Inferred from Sequence Orthology
more info
  
located_in secretory granule IDA
Inferred from Direct Assay
more info
 PubMed 
part_of serine protease inhibitor complex ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
tissue-type plasminogen activator
Names
t-plasminogen activator
NP_032898.2
XP_036009719.1
XP_036009720.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_008872.3 → NP_032898.2  tissue-type plasminogen activator preproprotein

    See identical proteins and their annotated locations for NP_032898.2

    Status: REVIEWED

    Source sequence(s)
    AC121835, AK135965, CJ146601, DV644940
    Consensus CDS
    CCDS22183.1
    UniProtKB/Swiss-Prot
    P11214, Q6P7U0, Q91VP2
    Related
    ENSMUSP00000033941.6, ENSMUST00000033941.7
    Conserved Domains (5) summary
    smart00058
    Location:38 → 80
    FN1; Fibronectin type 1 domain
    cd00108
    Location:210 → 295
    KR; Kringle domain; Kringle domains are believed to play a role in binding mediators, such as peptides, other proteins, membranes, or phospholipids. They are autonomous structural domains, found in a varying number of copies, in blood clotting and ...
    cd00190
    Location:311 → 556
    Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...
    pfam00008
    Location:83 → 115
    EGF; EGF-like domain
    pfam00051
    Location:124 → 205
    Kringle; Kringle domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000074.7 Reference GRCm39 C57BL/6J

    Range
    23247655..23272864
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036153827.1 → XP_036009720.1  tissue-type plasminogen activator isoform X2

    Conserved Domains (3) summary
    smart00058
    Location:38 → 80
    FN1; Fibronectin type 1 domain
    cd00108
    Location:84 → 169
    KR; Kringle domain; Kringle domains are believed to play a role in binding mediators, such as peptides, other proteins, membranes, or phospholipids. They are autonomous structural domains, found in a varying number of copies, in blood clotting and ...
    cd00190
    Location:185 → 430
    Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...

  2. XM_036153826.1 → XP_036009719.1  tissue-type plasminogen activator isoform X1

    UniProtKB/Swiss-Prot
    P11214, Q6P7U0, Q91VP2
    Conserved Domains (5) summary
    smart00058
    Location:38 → 80
    FN1; Fibronectin type 1 domain
    cd00108
    Location:210 → 295
    KR; Kringle domain; Kringle domains are believed to play a role in binding mediators, such as peptides, other proteins, membranes, or phospholipids. They are autonomous structural domains, found in a varying number of copies, in blood clotting and ...
    cd00190
    Location:311 → 556
    Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...
    pfam00008
    Location:83 → 115
    EGF; EGF-like domain
    pfam00051
    Location:124 → 205
    Kringle; Kringle domain
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P11214.3 GenPept UniProtKB/Swiss-Prot:P11214

Gene LinkOut

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