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BMC Physiol. 2018 Jan 25;18(1):2. doi: 10.1186/s12899-018-0036-0.

Regulation of Locomotor activity in fed, fasted, and food-restricted mice lacking tissue-type plasminogen activator.

Author information

1
Department of Biological Sciences, Kent State University, Kent, OH, 44242, USA.
2
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, 77030, USA.
3
Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, TN, 37996, USA.
4
Department of Biological Sciences, Kent State University, Kent, OH, 44242, USA. emintz@kent.edu.

Abstract

BACKGROUND:

Circadian rhythms of physiology and behavior are driven by a circadian clock located in the suprachiasmatic nucleus of the hypothalamus. This clock is synchronized to environmental day/night cycles by photic input, which is dependent on the presence of mature brain-derived neurotrophic factor (BDNF) in the SCN. Mature BDNF is produced by the enzyme plasmin, which is converted from plasminogen by the enzyme tissue-type plasminogen activator (tPA). In this study, we evaluate circadian function in mice lacking functional tPA.

RESULTS:

tPA-/- mice have normal circadian periods, but show decreased nocturnal wheel-running activity. This difference is eliminated or reversed on the second day of a 48-h fast. Similarly, when placed on daily cycles of restricted food availability the genotypic difference in total wheel-running activity disappears, and tPA-/- mice show equivalent amounts of food anticipatory activity to wild type mice.

CONCLUSIONS:

These data suggest that tPA regulates nocturnal wheel-running activity, and that tPA differentially affects SCN-driven nocturnal activity rhythms and activity driven by fasting or temporal food restriction.

KEYWORDS:

Circadian; Food anticipatory activity; Wheel-running

PMID:
29370799
PMCID:
PMC5784530
DOI:
10.1186/s12899-018-0036-0
[Indexed for MEDLINE]
Free PMC Article

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