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AP2B1 adaptor related protein complex 2 subunit beta 1 [ Homo sapiens (human) ]

Gene ID: 163, updated on 5-Jul-2020

Summary

Official Symbol
AP2B1provided by HGNC
Official Full Name
adaptor related protein complex 2 subunit beta 1provided by HGNC
Primary source
HGNC:HGNC:563
See related
Ensembl:ENSG00000006125 MIM:601025
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
ADTB2; AP105B; CLAPB1; AP2-BETA
Summary
The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Expression
Ubiquitous expression in brain (RPKM 64.4), testis (RPKM 50.1) and 25 other tissues See more
Orthologs

Genomic context

See AP2B1 in Genome Data Viewer
Location:
17q12
Exon count:
26
Annotation release Status Assembly Chr Location
109.20200522 current GRCh38.p13 (GCF_000001405.39) 17 NC_000017.11 (35587265..35726413)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 17 NC_000017.10 (33913918..34053436)

Chromosome 17 - NC_000017.11Genomic Context describing neighboring genes Neighboring gene small nucleolar RNA host gene 30 Neighboring gene small nucleolar RNA, C/D box 7 Neighboring gene peroxisomal biogenesis factor 12 Neighboring gene transmembrane protein 160 pseudogene Neighboring gene ribosomal protein L17 pseudogene 42 Neighboring gene RAS like family 10 member B Neighboring gene growth arrest specific 2 like 2

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Envelope transmembrane glycoprotein gp41 env HIV-1 Env gp41 requires the conserved membrane-proximal AP2B1 binding site in the gp41 cytoplasmic tail to evade antibody-dependent cell-mediated cytotoxicity (ADCC) via membrane-proximal endocytosis PubMed
env Two independent motifs, a membrane proximal conserved GYxxO motif (residues 711-715) and a C-terminal dileucine motif (residues 855-856), of the HIV-1 gp41 protein mediate endocytosis through interaction with the clathrin adaptor AP-2 PubMed
env The cytoplasmic domain (residues 707-856) of HIV-1 gp41 interacts with whole clathrin-associated AP-1 and AP-2 adaptor complexes PubMed
Nef nef Clathrin and the clathrin-associated, plasma membrane-localized AP2 complexes are required for the HIV-1 Nef-mediated downregulation of CD4 PubMed
nef A dileucine-motif-containing segment of Nef binds directly to the beta subunit of the clathrin adaptor complexes AP-2, which are responsible for recruiting sorted proteins into clathrin coated pits PubMed
nef HIV-1 Nef co-localizes with the beta subunit of the adaptor protein complexes (AP-2) in human T cells; Nef residues 174-179 are required for the co-localization of Nef with the beta subunit PubMed
nef In cells expressing an HIV-1 Nef-green fluorescent protein (GFP) fusion, CD28 co-localizes with the AP-2 clathrin adaptor and Nef-GFP PubMed
Pr55(Gag) gag HIV-1 Gag binds to AP-2, and this binding is dependent on tyrosine residue 132 and valine residue 135 at the matrix-capsid junction in the Gag polyprotein PubMed
Tat tat Both HIV-1 Tat 47-59 and FITC-labeled Tat 47-59 peptides upregulate gene expression of adaptor-related protein complex 2, beta 1 subunit (AP2B1) in U-937 macrophages PubMed
tat HIV-1 Tat enters T cells by using clathrin/AP2-mediated endocytosis followed by low-pH-induced and Hsp90-assisted endosomal translocation, which leads to cell responses that are induced from the cytosol PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • DKFZp781K0743

Gene Ontology Provided by GOA

Function Evidence Code Pubs
contributes_to clathrin adaptor activity TAS
Traceable Author Statement
more info
PubMed 
clathrin binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
clathrin binding IDA
Inferred from Direct Assay
more info
PubMed 
clathrin binding IPI
Inferred from Physical Interaction
more info
PubMed 
clathrin binding TAS
Traceable Author Statement
more info
PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein-containing complex binding IEA
Inferred from Electronic Annotation
more info
 
signal sequence binding TAS
Traceable Author Statement
more info
PubMed 
Process Evidence Code Pubs
Wnt signaling pathway, planar cell polarity pathway TAS
Traceable Author Statement
more info
 
antigen processing and presentation of exogenous peptide antigen via MHC class II TAS
Traceable Author Statement
more info
 
aorta development IEA
Inferred from Electronic Annotation
more info
 
clathrin coat assembly IEA
Inferred from Electronic Annotation
more info
 
clathrin-dependent endocytosis IDA
Inferred from Direct Assay
more info
PubMed 
clathrin-dependent endocytosis TAS
Traceable Author Statement
more info
PubMed 
coronary vasculature development IEA
Inferred from Electronic Annotation
more info
 
ephrin receptor signaling pathway TAS
Traceable Author Statement
more info
 
intracellular protein transport IEA
Inferred from Electronic Annotation
more info
 
low-density lipoprotein particle clearance TAS
Traceable Author Statement
more info
 
low-density lipoprotein particle receptor catabolic process TAS
Traceable Author Statement
more info
 
membrane organization TAS
Traceable Author Statement
more info
 
negative regulation of neuron death IEA
Inferred from Electronic Annotation
more info
 
positive regulation of endocytosis IEA
Inferred from Electronic Annotation
more info
 
positive regulation of protein localization to membrane IEA
Inferred from Electronic Annotation
more info
 
postsynaptic neurotransmitter receptor internalization IEA
Inferred from Electronic Annotation
more info
 
regulation of defense response to virus by virus TAS
Traceable Author Statement
more info
 
ventricular septum development IEA
Inferred from Electronic Annotation
more info
 
vesicle-mediated transport IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
Component Evidence Code Pubs
AP-2 adaptor complex IDA
Inferred from Direct Assay
more info
PubMed 
AP-2 adaptor complex IMP
Inferred from Mutant Phenotype
more info
PubMed 
AP-2 adaptor complex TAS
Traceable Author Statement
more info
PubMed 
clathrin adaptor complex IDA
Inferred from Direct Assay
more info
PubMed 
clathrin-coated endocytic vesicle NAS
Non-traceable Author Statement
more info
PubMed 
clathrin-coated endocytic vesicle membrane TAS
Traceable Author Statement
more info
 
cytosol TAS
Traceable Author Statement
more info
 
endocytic vesicle membrane TAS
Traceable Author Statement
more info
 
endolysosome membrane TAS
Traceable Author Statement
more info
 
glutamatergic synapse IEA
Inferred from Electronic Annotation
more info
 
membrane HDA PubMed 
plasma membrane TAS
Traceable Author Statement
more info
 
postsynapse IEA
Inferred from Electronic Annotation
more info
 

General protein information

Preferred Names
AP-2 complex subunit beta
Names
adapter-related protein complex 2 beta subunit
adapter-related protein complex 2 subunit beta
adaptin, beta 2 (beta)
adaptor protein complex AP-2 subunit beta
adaptor related protein complex 2 beta 1 subunit
adaptor-related protein complex 2 subunit beta
beta-2-adaptin
beta-adaptin
clathrin assembly protein complex 2 beta large chain
clathrin-associated/assembly/adaptor protein, large, beta 1
plasma membrane adaptor HA2/AP2 adaptin beta subunit
testicular tissue protein Li 22

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001030006.2NP_001025177.1  AP-2 complex subunit beta isoform a

    See identical proteins and their annotated locations for NP_001025177.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).
    Source sequence(s)
    AI691147, BC006201, BC012150, BQ421703, BX643620, M34175
    Consensus CDS
    CCDS32621.1
    UniProtKB/Swiss-Prot
    P63010
    UniProtKB/TrEMBL
    A0A140VJE8, Q96EL6
    Related
    ENSP00000483185.1, ENST00000610402.5
    Conserved Domains (4) summary
    smart00809
    Location:731831
    Alpha_adaptinC2; Adaptin C-terminal domain
    smart01020
    Location:840950
    B2-adapt-app_C; Beta2-adaptin appendage, C-terminal sub-domain
    sd00044
    Location:5175
    HEAT; HEAT repeat [structural motif]
    pfam01602
    Location:11534
    Adaptin_N; Adaptin N terminal region
  2. NM_001282.3NP_001273.1  AP-2 complex subunit beta isoform b

    See identical proteins and their annotated locations for NP_001273.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) lacks an in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.
    Source sequence(s)
    AI691147, BQ421703, BX643620, M34175
    Consensus CDS
    CCDS32622.1
    UniProtKB/Swiss-Prot
    P63010
    Related
    ENSP00000482315.1, ENST00000621914.4
    Conserved Domains (4) summary
    smart00809
    Location:717817
    Alpha_adaptinC2; Adaptin C-terminal domain
    smart01020
    Location:826936
    B2-adapt-app_C; Beta2-adaptin appendage, C-terminal sub-domain
    sd00044
    Location:5175
    HEAT; HEAT repeat [structural motif]
    pfam01602
    Location:11534
    Adaptin_N; Adaptin N terminal region

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000017.11 Reference GRCh38.p13 Primary Assembly

    Range
    35587265..35726413
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011524452.1XP_011522754.1  AP-2 complex subunit beta isoform X5

    See identical proteins and their annotated locations for XP_011522754.1

    Conserved Domains (5) summary
    smart00809
    Location:674768
    Alpha_adaptinC2; Adaptin C-terminal domain
    smart01020
    Location:815925
    B2-adapt-app_C; Beta2-adaptin appendage, C-terminal sub-domain
    pfam01602
    Location:1477
    Adaptin_N; Adaptin N terminal region
    pfam12717
    Location:42211
    Cnd1; non-SMC mitotic condensation complex subunit 1
    sd00044
    Location:3057
    HEAT; HEAT repeat [structural motif]
  2. XM_017024285.1XP_016879774.1  AP-2 complex subunit beta isoform X6

  3. XM_011524448.2XP_011522750.1  AP-2 complex subunit beta isoform X1

    See identical proteins and their annotated locations for XP_011522750.1

    Conserved Domains (4) summary
    smart00809
    Location:731825
    Alpha_adaptinC2; Adaptin C-terminal domain
    smart01020
    Location:872982
    B2-adapt-app_C; Beta2-adaptin appendage, C-terminal sub-domain
    sd00044
    Location:5175
    HEAT; HEAT repeat [structural motif]
    pfam01602
    Location:11534
    Adaptin_N; Adaptin N terminal region
  4. XM_011524451.2XP_011522753.1  AP-2 complex subunit beta isoform X2

    Conserved Domains (4) summary
    smart00809
    Location:717811
    Alpha_adaptinC2; Adaptin C-terminal domain
    smart01020
    Location:858968
    B2-adapt-app_C; Beta2-adaptin appendage, C-terminal sub-domain
    sd00044
    Location:5175
    HEAT; HEAT repeat [structural motif]
    pfam01602
    Location:11534
    Adaptin_N; Adaptin N terminal region
  5. XM_005257941.3XP_005257998.1  AP-2 complex subunit beta isoform X8

    Related
    ENSP00000478471.1, ENST00000616784.4
    Conserved Domains (3) summary
    pfam01602
    Location:11534
    Adaptin_N; Adaptin N terminal region
    pfam12717
    Location:99268
    Cnd1; non-SMC mitotic condensation complex subunit 1
    sd00044
    Location:5175
    HEAT; HEAT repeat [structural motif]
  6. XM_011524453.1XP_011522755.1  AP-2 complex subunit beta isoform X5

    See identical proteins and their annotated locations for XP_011522755.1

    Conserved Domains (5) summary
    smart00809
    Location:674768
    Alpha_adaptinC2; Adaptin C-terminal domain
    smart01020
    Location:815925
    B2-adapt-app_C; Beta2-adaptin appendage, C-terminal sub-domain
    pfam01602
    Location:1477
    Adaptin_N; Adaptin N terminal region
    pfam12717
    Location:42211
    Cnd1; non-SMC mitotic condensation complex subunit 1
    sd00044
    Location:3057
    HEAT; HEAT repeat [structural motif]
  7. XM_011524455.2XP_011522757.1  AP-2 complex subunit beta isoform X7

    See identical proteins and their annotated locations for XP_011522757.1

    UniProtKB/Swiss-Prot
    P63010
    Conserved Domains (4) summary
    smart00809
    Location:660760
    Alpha_adaptinC2; Adaptin C-terminal domain
    smart01020
    Location:769879
    B2-adapt-app_C; Beta2-adaptin appendage, C-terminal sub-domain
    sd00044
    Location:3057
    HEAT; HEAT repeat [structural motif]
    pfam01602
    Location:1477
    Adaptin_N; Adaptin N terminal region
  8. XM_011524449.3XP_011522751.1  AP-2 complex subunit beta isoform X1

    See identical proteins and their annotated locations for XP_011522751.1

    Conserved Domains (4) summary
    smart00809
    Location:731825
    Alpha_adaptinC2; Adaptin C-terminal domain
    smart01020
    Location:872982
    B2-adapt-app_C; Beta2-adaptin appendage, C-terminal sub-domain
    sd00044
    Location:5175
    HEAT; HEAT repeat [structural motif]
    pfam01602
    Location:11534
    Adaptin_N; Adaptin N terminal region
  9. XM_005257937.4XP_005257994.1  AP-2 complex subunit beta isoform X3

    See identical proteins and their annotated locations for XP_005257994.1

    UniProtKB/Swiss-Prot
    P63010
    UniProtKB/TrEMBL
    A0A140VJE8
    Related
    ENSP00000484708.1, ENST00000616681.4
    Conserved Domains (4) summary
    smart00809
    Location:731831
    Alpha_adaptinC2; Adaptin C-terminal domain
    smart01020
    Location:840950
    B2-adapt-app_C; Beta2-adaptin appendage, C-terminal sub-domain
    sd00044
    Location:5175
    HEAT; HEAT repeat [structural motif]
    pfam01602
    Location:11534
    Adaptin_N; Adaptin N terminal region
  10. XM_017024284.2XP_016879773.1  AP-2 complex subunit beta isoform X4

    UniProtKB/Swiss-Prot
    P63010
    Conserved Domains (4) summary
    smart00809
    Location:717817
    Alpha_adaptinC2; Adaptin C-terminal domain
    smart01020
    Location:826936
    B2-adapt-app_C; Beta2-adaptin appendage, C-terminal sub-domain
    sd00044
    Location:5175
    HEAT; HEAT repeat [structural motif]
    pfam01602
    Location:11534
    Adaptin_N; Adaptin N terminal region
  11. XM_017024287.2XP_016879776.1  AP-2 complex subunit beta isoform X8

    Related
    ENSP00000479691.1, ENST00000614600.4
    Conserved Domains (3) summary
    pfam01602
    Location:11534
    Adaptin_N; Adaptin N terminal region
    pfam12717
    Location:99268
    Cnd1; non-SMC mitotic condensation complex subunit 1
    sd00044
    Location:5175
    HEAT; HEAT repeat [structural motif]
  12. XM_017024286.1XP_016879775.1  AP-2 complex subunit beta isoform X7

    UniProtKB/Swiss-Prot
    P63010
    Conserved Domains (4) summary
    smart00809
    Location:660760
    Alpha_adaptinC2; Adaptin C-terminal domain
    smart01020
    Location:769879
    B2-adapt-app_C; Beta2-adaptin appendage, C-terminal sub-domain
    sd00044
    Location:3057
    HEAT; HEAT repeat [structural motif]
    pfam01602
    Location:1477
    Adaptin_N; Adaptin N terminal region
  13. XM_011524450.2XP_011522752.1  AP-2 complex subunit beta isoform X1

    See identical proteins and their annotated locations for XP_011522752.1

    Conserved Domains (4) summary
    smart00809
    Location:731825
    Alpha_adaptinC2; Adaptin C-terminal domain
    smart01020
    Location:872982
    B2-adapt-app_C; Beta2-adaptin appendage, C-terminal sub-domain
    sd00044
    Location:5175
    HEAT; HEAT repeat [structural motif]
    pfam01602
    Location:11534
    Adaptin_N; Adaptin N terminal region
  14. XM_005257938.3XP_005257995.1  AP-2 complex subunit beta isoform X3

    See identical proteins and their annotated locations for XP_005257995.1

    UniProtKB/Swiss-Prot
    P63010
    UniProtKB/TrEMBL
    A0A140VJE8
    Related
    ENSP00000482835.1, ENST00000618940.4
    Conserved Domains (4) summary
    smart00809
    Location:731831
    Alpha_adaptinC2; Adaptin C-terminal domain
    smart01020
    Location:840950
    B2-adapt-app_C; Beta2-adaptin appendage, C-terminal sub-domain
    sd00044
    Location:5175
    HEAT; HEAT repeat [structural motif]
    pfam01602
    Location:11534
    Adaptin_N; Adaptin N terminal region
  15. XM_011524454.1XP_011522756.1  AP-2 complex subunit beta isoform X5

    See identical proteins and their annotated locations for XP_011522756.1

    Conserved Domains (5) summary
    smart00809
    Location:674768
    Alpha_adaptinC2; Adaptin C-terminal domain
    smart01020
    Location:815925
    B2-adapt-app_C; Beta2-adaptin appendage, C-terminal sub-domain
    pfam01602
    Location:1477
    Adaptin_N; Adaptin N terminal region
    pfam12717
    Location:42211
    Cnd1; non-SMC mitotic condensation complex subunit 1
    sd00044
    Location:3057
    HEAT; HEAT repeat [structural motif]
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