SSRP1 structure specific recognition protein 1 [Homo sapiens (human)] - Gene

Summary

Official Symbol: SSRP1provided by HGNC
Official Full Name: structure specific recognition protein 1provided by HGNC
Primary source: HGNC:HGNC:11327
See related: Ensembl:ENSG00000149136 MIM:604328; AllianceGenome:HGNC:11327
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: FACT; T160; FACT80
Summary: The protein encoded by this gene is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63. An alternatively spliced transcript variant of this gene has been described, but its full-length nature is not known. [provided by RefSeq, Jul 2008]
Expression: Ubiquitous expression in lymph node (RPKM 33.5), ovary (RPKM 32.7) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 11q12.1

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	11	NC_000011.10 (57325988..57335892, complement)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	11	NC_060935.1 (57275479..57285383, complement)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	11	NC_000011.9 (57093462..57103366, complement)

Chromosome 11 - NC_000011.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Hypoxia-inducible factor 1 recruits FACT and RNF20/40 to mediate histone ubiquitination and transcriptional activation of target genes.
Title: Hypoxia-inducible factor 1 recruits FACT and RNF20/40 to mediate histone ubiquitination and transcriptional activation of target genes.
Histone chaperone SSRP1 is required for apoptosis inhibition and mitochondrial function in HCC via transcriptional promotion of TRAP1.
Title: Histone chaperone SSRP1 is required for apoptosis inhibition and mitochondrial function in HCC via transcriptional promotion of TRAP1.
Human FACT subunits coordinate to catalyze both disassembly and reassembly of nucleosomes.
Title: Human FACT subunits coordinate to catalyze both disassembly and reassembly of nucleosomes.
Electron microscopy analysis of ATP-independent nucleosome unfolding by FACT.
Title: Electron microscopy analysis of ATP-independent nucleosome unfolding by FACT.
Dual targeting of the epigenome via FACT complex and histone deacetylase is a potent treatment strategy for DIPG.
Title: Dual targeting of the epigenome via FACT complex and histone deacetylase is a potent treatment strategy for DIPG.
Structural insights into multifunctionality of human FACT complex subunit hSSRP1.
Title: Structural insights into multifunctionality of human FACT complex subunit hSSRP1.
Histone chaperone FACT complex inhibitor CBL0137 interferes with DNA damage repair and enhances sensitivity of medulloblastoma to chemotherapy and radiation.
Title: Histone chaperone FACT complex inhibitor CBL0137 interferes with DNA damage repair and enhances sensitivity of medulloblastoma to chemotherapy and radiation.
SSRP1 Is a Prognostic Biomarker Correlated with CD8(+) T Cell Infiltration in Hepatocellular Carcinoma (HCC).
Title: SSRP1 Is a Prognostic Biomarker Correlated with CD8(+) T Cell Infiltration in Hepatocellular Carcinoma (HCC).
CPF impedes cell cycle re-entry of quiescent lung cancer cells through transcriptional suppression of FACT and c-MYC.
Title: CPF impedes cell cycle re-entry of quiescent lung cancer cells through transcriptional suppression of FACT and c-MYC.
The role of FACT in managing chromatin: disruption, assembly, or repair?
Title: The role of FACT in managing chromatin: disruption, assembly, or repair?

Submit: New GeneRIF Correction See all GeneRIFs (55)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Replication interactions

Interaction	Pubs
HIV-1 infection requires SSRP1 as shown through shRNA-mediated knockdown of SSRP1 in human CD4+ T cells	PubMed
shRNA knockdown of SSRP1 increases HIV-1 LTR-driven gene expression in HEK293 cells transduced with VSV-G pseudo-typed HIV-1 NL4-3-Luc construct; i.e. HIV-1 is restricted by SSRP1	PubMed

Protein interactions

Protein	Gene	Interaction	Pubs
Tat	tat	The heterodimeric complex FACT proteins SPT16 and SSRP1 are recognized to interact with HIV-1 in Jurkat cell	PubMed
	tat	HIV-1 Tat activated transcription facilitates removal of histones H2A and H2B at the LTR, and that the FACT complex, at least consisting of SPT16 and SSRP1, may be responsible for their removal	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH47161 (e-PCR)
- UniSTS:9514

GDB:197864 (e-PCR)
- UniSTS:155978

RH17745 (e-PCR)
- UniSTS:64877

SSRP1 (e-PCR)
- UniSTS:14484

SHGC-30227 (e-PCR)
- UniSTS:81589

WI-9146 (e-PCR)
- UniSTS:32975

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables RNA binding	HDA more info	PubMed
enables histone binding	IBA Inferred from Biological aspect of Ancestor more info
enables nucleosome binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in nucleosome assembly	NAS Non-traceable Author Statement more info	PubMed
involved_in nucleosome disassembly	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of chromatin organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of chromatin organization	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of FACT complex	IBA Inferred from Biological aspect of Ancestor more info
part_of FACT complex	IPI Inferred from Physical Interaction more info	PubMed
located_in nucleolus	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: FACT complex subunit SSRP1

Names: FACT 80 kDa subunit; FACTp80; chromatin-specific transcription elongation factor 80 kDa subunit; cisplatin-DNA SSRP; facilitates chromatin remodeling 80 kDa subunit; facilitates chromatin transcription complex 80 kDa subunit; facilitates chromatin transcription complex subunit SSRP1; hSSRP1; high mobility group box; recombination signal sequence recognition protein 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_003146.3 → NP_003137.1 FACT complex subunit SSRP1

See identical proteins and their annotated locations for NP_003137.1

Status: REVIEWED

Source sequence(s)

AP000781

Consensus CDS

CCDS7952.1

UniProtKB/Swiss-Prot

Q08945, Q5BJG8

Related

ENSP00000278412.2, ENST00000278412.7

Conserved Domains (2) summary

COG5165
Location:21 → 480

POB3; Nucleosome-binding factor SPN, POB3 subunit [Transcription / DNA replication, recombination, and repair / Chromatin structure and dynamics]

pfam00505
Location:547 → 614

HMG_box; HMG (high mobility group) box

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000011.10 Reference GRCh38.p14 Primary Assembly

Range

57325988..57335892 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_047427498.1 → XP_047283454.1 FACT complex subunit SSRP1 isoform X2

UniProtKB/Swiss-Prot

Q08945, Q5BJG8
XM_017018180.2 → XP_016873669.2 FACT complex subunit SSRP1 isoform X1
XM_047427499.1 → XP_047283455.1 FACT complex subunit SSRP1 isoform X3

Alternate T2T-CHM13v2.0

Genomic

NC_060935.1 Alternate T2T-CHM13v2.0

Range

57275479..57285383 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_054369757.1 → XP_054225732.1 FACT complex subunit SSRP1 isoform X2

UniProtKB/Swiss-Prot

Q08945, Q5BJG8
XM_054369756.1 → XP_054225731.1 FACT complex subunit SSRP1 isoform X1
XM_054369758.1 → XP_054225733.1 FACT complex subunit SSRP1 isoform X3