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    Cyb5r4 cytochrome b5 reductase 4 [ Rattus norvegicus (Norway rat) ]

    Gene ID: 171015, updated on 11-Apr-2024

    Summary

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    Official Symbol
    Cyb5r4provided by RGD
    Official Full Name
    cytochrome b5 reductase 4provided by RGD
    Primary source
    RGD:621834
    See related
    Ensembl:ENSRNOG00000010024 AllianceGenome:RGD:621834
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Rattus norvegicus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
    Also known as
    Ncb5or; b5&b5R; b5+b5R; cb5/cb5R
    Summary
    Enables NADPH-hemoprotein reductase activity; flavin adenine dinucleotide binding activity; and heme binding activity. Involved in NADP metabolic process. Predicted to be located in perinuclear region of cytoplasm. Predicted to be active in endoplasmic reticulum. Human ortholog(s) of this gene implicated in type 2 diabetes mellitus. Orthologous to human CYB5R4 (cytochrome b5 reductase 4). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Biased expression in Kidney (RPKM 180.1), Thymus (RPKM 149.1) and 9 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    8q31
    Exon count:
    16
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCr8 (GCF_036323735.1) 8 NC_086026.1 (96860415..96925789)
    RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 8 NC_051343.1 (87975596..88048356)
    106 previous assembly Rnor_6.0 (GCF_000001895.5) 8 NC_005107.4 (94693273..94757989)

    Chromosome 8 - NC_086026.1Genomic Context describing neighboring genes Neighboring gene synaptosome associated protein 91 Neighboring gene ripply transcriptional repressor 2 Neighboring gene melanocortin 2 receptor accessory protein 2 Neighboring gene centrosomal protein 162

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
    • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
    • BioProject: PRJNA238328
    • Publication: PMID 24510058
    • Analysis date: Mon Jun 6 17:44:12 2016

    Bibliography

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    Related articles in PubMed

    1. Mutagenesis of Glycine 179 modulates both catalytic efficiency and reduced pyridine nucleotide specificity in cytochrome b5 reductase. Roma GW, et al. Biochemistry, 2005 Oct 18. PMID 16216070
    2. The reductase NCB5OR is responsive to the redox status in beta-cells and is not involved in the ER stress response. Larade K, et al. Biochem J, 2007 Jun 15. PMID 17343567, Free PMC Article
    3. Variation in NCB5OR: studies of relationships to type 2 diabetes, maturity-onset diabetes of the young, and gestational diabetes mellitus. Andersen G, et al. Diabetes, 2004 Nov. PMID 15504981, Free PMC Article
    4. Absence of a reductase, NCB5OR, causes insulin-deficient diabetes. Xie J, et al. Proc Natl Acad Sci U S A, 2004 Jul 20. PMID 15247412, Free PMC Article
    5. Identification and characterization of a novel splice variant of mouse and rat cytochrome b5/cytochrome b5 reductase. Curry BJ, et al. Genomics, 2004 Mar. PMID 14962668

    See all (10) citations in PubMed

    GeneRIFs: Gene References Into Functions

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by RGD

    Function Evidence Code Pubs
    enables NAD(P)H oxidase H2O2-forming activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    NOT enables NAD(P)H oxidase H2O2-forming activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables NAD(P)H oxidase H2O2-forming activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables NADPH-hemoprotein reductase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables cytochrome-b5 reductase activity, acting on NAD(P)H IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables cytochrome-b5 reductase activity, acting on NAD(P)H IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cytochrome-b5 reductase activity, acting on NAD(P)H ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables flavin adenine dinucleotide binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables heme binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables heme binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables heme binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in NADP metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in cell development IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within cell development ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in glucose homeostasis IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within glucose homeostasis ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in insulin secretion IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within insulin secretion ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in reactive oxygen species metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in response to antibiotic IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within response to antibiotic ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in superoxide metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in superoxide metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in superoxide metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in endoplasmic reticulum IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in endoplasmic reticulum IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in endoplasmic reticulum ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in perinuclear region of cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in perinuclear region of cytoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    cytochrome b5 reductase 4
    Names
    N-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containing protein
    NADPH cytochrome B5 oxidoreductase
    flavohemoprotein b5+b5R
    flavohemoprotein b5/b5R
    NP_596918.3
    XP_006243534.1
    XP_017450917.1
    XP_038936668.1
    XP_063120880.1
    XP_063120881.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_133427.3 → NP_596918.3  cytochrome b5 reductase 4

      See identical proteins and their annotated locations for NP_596918.3

      Status: VALIDATED

      Source sequence(s)
      BC080240, JAXUCZ010000008
      UniProtKB/Swiss-Prot
      Q68EJ0, Q6VXY2, Q6VXY3, Q9EPZ4
      UniProtKB/TrEMBL
      A0A0G2JSK2, A6I1W7
      Related
      ENSRNOP00000013909.4, ENSRNOT00000013908.6
      Conserved Domains (3) summary
      cd06183
      Location:277 → 518
      cyt_b5_reduct_like; Cytochrome b5 reductase catalyzes the reduction of 2 molecules of cytochrome b5 using NADH as an electron donor. Like ferredoxin reductases, these proteins have an N-terminal FAD binding subdomain and a C-terminal NADH binding subdomain, separated by a ...
      cd06490
      Location:169 → 255
      p23_NCB5OR; p23_like domain found in NAD(P)H cytochrome b5 (NCB5) oxidoreductase (OR) and similar proteins. NCB5OR is widely expressed in human organs and tissues and is localized in the ER (endoplasmic reticulum). It appears to play a critical role in maintaining ...
      pfam00173
      Location:56 → 128
      Cyt-b5; Cytochrome b5-like Heme/Steroid binding domain

    RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCr8

    Genomic

    1. NC_086026.1 Reference GRCr8

      Range
      96860415..96925789
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_039080740.2 → XP_038936668.1  cytochrome b5 reductase 4 isoform X2

      UniProtKB/TrEMBL
      A6I1W6
      Conserved Domains (3) summary
      cd06183
      Location:243 → 484
      cyt_b5_reduct_like; Cytochrome b5 reductase catalyzes the reduction of 2 molecules of cytochrome b5 using NADH as an electron donor. Like ferredoxin reductases, these proteins have an N-terminal FAD binding subdomain and a C-terminal NADH binding subdomain, separated by a ...
      cd06490
      Location:135 → 221
      p23_NCB5OR; p23_like domain found in NAD(P)H cytochrome b5 (NCB5) oxidoreductase (OR) and similar proteins. NCB5OR is widely expressed in human organs and tissues and is localized in the ER (endoplasmic reticulum). It appears to play a critical role in maintaining ...
      pfam00173
      Location:24 → 96
      Cyt-b5; Cytochrome b5-like Heme/Steroid binding domain

    2. XM_063264810.1 → XP_063120880.1  cytochrome b5 reductase 4 isoform X4

    3. XM_017595428.3 → XP_017450917.1  cytochrome b5 reductase 4 isoform X3

      UniProtKB/Swiss-Prot
      Q68EJ0, Q6VXY2, Q6VXY3, Q9EPZ4
      UniProtKB/TrEMBL
      A0A8I6GKQ4
      Related
      ENSRNOP00000093571.1

    4. XM_006243472.5 → XP_006243534.1  cytochrome b5 reductase 4 isoform X1

      UniProtKB/Swiss-Prot
      Q68EJ0, Q6VXY2, Q6VXY3, Q9EPZ4
      UniProtKB/TrEMBL
      A0A8I6A4B1
      Related
      ENSRNOP00000085891.1
      Conserved Domains (3) summary
      cd06183
      Location:267 → 508
      cyt_b5_reduct_like; Cytochrome b5 reductase catalyzes the reduction of 2 molecules of cytochrome b5 using NADH as an electron donor. Like ferredoxin reductases, these proteins have an N-terminal FAD binding subdomain and a C-terminal NADH binding subdomain, separated by a ...
      cd06490
      Location:159 → 245
      p23_NCB5OR; p23_like domain found in NAD(P)H cytochrome b5 (NCB5) oxidoreductase (OR) and similar proteins. NCB5OR is widely expressed in human organs and tissues and is localized in the ER (endoplasmic reticulum). It appears to play a critical role in maintaining ...
      pfam00173
      Location:56 → 128
      Cyt-b5; Cytochrome b5-like Heme/Steroid binding domain

    5. XM_063264811.1 → XP_063120881.1  cytochrome b5 reductase 4 isoform X5

    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q68EJ0.2 GenPept UniProtKB/Swiss-Prot:Q68EJ0

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