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    EIF5B eukaryotic translation initiation factor 5B [ Homo sapiens (human) ]

    Gene ID: 9669, updated on 7-Apr-2024

    Summary

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    Official Symbol
    EIF5Bprovided by HGNC
    Official Full Name
    eukaryotic translation initiation factor 5Bprovided by HGNC
    Primary source
    HGNC:HGNC:30793
    See related
    Ensembl:ENSG00000158417 MIM:606086; AllianceGenome:HGNC:30793
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    IF2
    Summary
    Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq, Jul 2008]
    Expression
    Ubiquitous expression in heart (RPKM 26.2), testis (RPKM 23.3) and 25 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    Location:
    2q11.2
    Exon count:
    24
    Annotation release Status Assembly Chr Location
    RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 2 NC_000002.12 (99337389..99401326)
    RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 2 NC_060926.1 (99796021..99859976)
    105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 2 NC_000002.11 (99953852..100017789)

    Chromosome 2 - NC_000002.12Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC107985923 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16281 Neighboring gene H3K27ac hESC enhancer GRCh37_chr2:99952564-99953104 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16284 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16285 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16286 Neighboring gene H3K27ac hESC enhancer GRCh37_chr2:99953733-99954234 Neighboring gene H3K27ac hESC enhancer GRCh37_chr2:99954235-99954734 Neighboring gene thioredoxin domain containing 9 Neighboring gene lysozyme g1 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr2:100019841-100021040 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 11808 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 11809 Neighboring gene OCT4-NANOG hESC enhancer GRCh37_chr2:100056357-100057026 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:100092059-100092722 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16288 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 11810 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 11811 Neighboring gene H3K27ac hESC enhancer GRCh37_chr2:100106417-100107302 Neighboring gene REV1 DNA directed polymerase Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16289 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr2:100146131-100146758 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr2:100170377-100170925 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr2:100170926-100171473 Neighboring gene NANOG hESC enhancer GRCh37_chr2:100171857-100172363 Neighboring gene NANOG hESC enhancer GRCh37_chr2:100188674-100189175 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr2:100218069-100218631 Neighboring gene ALF transcription elongation factor 3 Neighboring gene uncharacterized LOC124906051 Neighboring gene uncharacterized LOC105373504

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. Dynamic interaction network involving the conserved intrinsically disordered regions in human eIF5. Paul EE, et al. Biophys Chem, 2022 Feb. PMID 34923394, Free PMC Article
    2. eIF5B drives integrated stress response-dependent translation of PD-L1 in lung cancer. Suresh S, et al. Nat Cancer, 2020 May. PMID 32984844, Free PMC Article
    3. Eukaryotic initiation factor 5B (eIF5B) regulates temozolomide-mediated apoptosis in brain tumour stem cells (BTSCs). Ross JA, et al. Biochem Cell Biol, 2020 Dec. PMID 31671279
    4. Eukaryotic initiation factor 5B (eIF5B) provides a critical cell survival switch to glioblastoma cells via regulation of apoptosis. Ross JA, et al. Cell Death Dis, 2019 Jan 22. PMID 30670698, Free PMC Article
    5. Oxygen-Sensitive Remodeling of Central Carbon Metabolism by Archaic eIF5B. Ho JJD, et al. Cell Rep, 2018 Jan 2. PMID 29298419, Free PMC Article

    See all (147) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. eIF5B and eIF1A reorient initiator tRNA to allow ribosomal subunit joining.
      Title: eIF5B and eIF1A reorient initiator tRNA to allow ribosomal subunit joining.
    2. eIF5B drives integrated stress response-dependent translation of PD-L1 in lung cancer.
      Title: eIF5B drives integrated stress response-dependent translation of PD-L1 in lung cancer.
    3. Dynamic interaction network involving the conserved intrinsically disordered regions in human eIF5.
      Title: Dynamic interaction network involving the conserved intrinsically disordered regions in human eIF5.
    4. Depletion of eukaryotic initiation factor 5B (eIF5B) reprograms the cellular transcriptome and leads to activation of endoplasmic reticulum (ER) stress and c-Jun N-terminal kinase (JNK).
      Title: Depletion of eukaryotic initiation factor 5B (eIF5B) reprograms the cellular transcriptome and leads to activation of endoplasmic reticulum (ER) stress and c-Jun N-terminal kinase (JNK).
    5. eIF5B regulates the expression of PD-L1 in prostate cancer cells by interacting with Wig1.
      Title: eIF5B regulates the expression of PD-L1 in prostate cancer cells by interacting with Wig1.
    6. Eukaryotic initiation factor 5B (eIF5B) regulates temozolomide-mediated apoptosis in brain tumour stem cells (BTSCs).
      Title: Eukaryotic initiation factor 5B (eIF5B) regulates temozolomide-mediated apoptosis in brain tumour stem cells (BTSCs).
    7. Data suggest that eIF5B represents a regulatory node, allowing cancer cells to evade apoptosis by promoting the translation of pro-survival proteins from IRES-containing mRNAs.
      Title: Eukaryotic initiation factor 5B (eIF5B) provides a critical cell survival switch to glioblastoma cells via regulation of apoptosis.
    8. results indicate that in humans, eIF5B displacing eIF2 from Met-tRNAi upon subunit joining may be coupled to eIF1A displacing eIF5 from eIF5B, allowing the eIF5:eIF2-GDP complex to leave the ribosome.
      Title: Human eIF5 and eIF1A Compete for Binding to eIF5B.
    9. aerobic eukarya retained eIF5B/IF2 to remodel anaerobic pathways during episodes of oxygen deficiency.
      Title: Oxygen-Sensitive Remodeling of Central Carbon Metabolism by Archaic eIF5B.
    10. eIF5B promoted hepatocellular carcinoma cell proliferation and migration in vitro and in vivo partly through increasing ASAP1 expression.
      Title: eIF5B increases ASAP1 expression to promote HCC proliferation and invasion.
    Submit: New GeneRIF Correction See all GeneRIFs (18)

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    EBI GWAS Catalog

    Description
    Large-scale genome-wide association analysis of bipolar disorder identifies a new susceptibility locus near ODZ4.
    EBI GWAS Catalog

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    Genotypes

    HIV-1 interactions

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    Protein interactions

    Protein Gene Interaction Pubs
    Pr55(Gag) gag HIV-1 Gag interacts with EIF5B as demonstrated by proximity dependent biotinylation proteomics PubMed
    matrix gag HIV-1 Matrix inhibits hIF2 mediated translation in a reticulocyte lysate assay PubMed
    gag HIV-1 Matrix, as well as the full length HIV-1 Gag protein bind to human translation initiation factor two (hIF2) and these proteins can be immunoprecipitated as a protein complex PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Clone Names

    • FLJ10524, KIAA0741, DKFZp434I036

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables GTP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables GTPase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables GTPase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables RNA binding HDA PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables tRNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables translation initiation factor activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables translation initiation factor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables translation initiation factor activity NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in regulation of translational initiation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of translational initiation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in ribosome assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in translational initiation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol TAS
    Traceable Author Statement
    more info
    		  
    NOT located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in synapse IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    eukaryotic translation initiation factor 5B
    Names
    eIF-5B
    translation initiation factor IF-2
    translation initiation factor IF2
    NP_056988.3

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_015904.4 → NP_056988.3  eukaryotic translation initiation factor 5B

      See identical proteins and their annotated locations for NP_056988.3

      Status: REVIEWED

      Source sequence(s)
      AB018284, AC018690, BC032639, BX485839
      Consensus CDS
      CCDS42721.1
      UniProtKB/Swiss-Prot
      O60841, O95805, Q53RV7, Q53SI8, Q9UF81, Q9UMN7
      UniProtKB/TrEMBL
      Q8N5A0
      Related
      ENSP00000289371.5, ENST00000289371.11
      Conserved Domains (6) summary
      cd01887
      Location:632 → 845
      IF2_eIF5B; Initiation Factor 2 (IF2)/ eukaryotic Initiation Factor 5B (eIF5B) family
      PRK04004
      Location:627 → 1202
      PRK04004; translation initiation factor IF-2; Validated
      pfam08070
      Location:110 → 171
      DTHCT; DTHCT (NUC029) region
      pfam11987
      Location:966 → 1067
      IF-2; Translation-initiation factor 2
      cd03703
      Location:855 → 965
      aeIF5B_II; Domain II of archaeal and eukaryotic Initiation Factor 5
      cd16266
      Location:1092 → 1186
      IF2_aeIF5B_IV; Domain IV of prokaryotic Initiation Factor 2 and archaeal and eukaryotic Initiation Factor 5

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000002.12 Reference GRCh38.p14 Primary Assembly

      Range
      99337389..99401326
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060926.1 Alternate T2T-CHM13v2.0

      Range
      99796021..99859976
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    O60841.4 GenPept UniProtKB/Swiss-Prot:O60841

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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