|
| Status |
Public on Feb 24, 2014 |
| Title |
221/4 |
| Sample type |
RNA |
| |
|
| Source name |
whole-blood leukocytes, etanercept, 4hr
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: whole-blood leukocytes
gender: male
disease status: healthy
treatment: etanercept
time: 4 hours
individual identifier: 221
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated in accordance with Qiagen protocols for the extraction of PAXgene tube-treated whole blood.
|
| Label |
biotin
|
| Label protocol |
Synthesis, amplification and purification of anti-sense RNA was performed using 300 ng of enriched mRNA per sample and the Illumina TotalPrep RNA Amplification Kit (Ambion/Applied Biosystems) following the Illumina Sentrix Array Matrix expression protocol.
|
| |
|
| Hybridization protocol |
A total of 750 ng biotinylated cRNA was hybridized (Ambion).
|
| Scan protocol |
The BeadChips were scanned according to the protocol described in the Illumina Whole Genome Gene Expression for BeadStation Manual v3.2, Revision A using scanning software BeadScan 3.5.31.
|
| Description |
4968529052_L
endotoxin
|
| Data processing |
Variance stabilization normalization was applied. The statistical software R and Bioconductor libraries lumi and vsn were used.
|
| |
|
| Submission date |
Feb 29, 2012 |
| Last update date |
Feb 24, 2014 |
| Contact name |
Brendon Scicluna |
| E-mail(s) |
brendon.scicluna@um.edu.mt
|
| Organization name |
University of Malta
|
| Street address |
Msida campus
|
| City |
Msida |
| ZIP/Postal code |
MSD2020 |
| Country |
Malta |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE36177 |
Genome-wide gene expression analysis of the whole blood leukocyte transcriptional response to endotoxin treatment |
|
