|
| Status |
Public on Feb 03, 2012 |
| Title |
male breast cancer 22 |
| Sample type |
RNA |
| |
|
| Source name |
male breast cancer
|
| Organism |
Homo sapiens |
| Characteristics |
age: 72
size [mm]: 24
nottingham histological grade (nhg): 2
er: positive
pr: positive
year of diagnosis: 1996
hybridization batch: 2
extraction date: 20081021
pathology department: 9
gender: male
tissue: breast cancer tumor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 22
|
| Data processing |
The data were normalised using quantile normalisation with BASE2 Combat adjusted one the hybridization batches
|
| |
|
| Submission date |
Aug 08, 2011 |
| Last update date |
Feb 03, 2012 |
| Contact name |
Ida Johansson |
| E-mail(s) |
ida.johansson@med.lu.se
|
| Organization name |
Lund University
|
| Department |
Oncology
|
| Street address |
Department of Oncology Clinical Sciences Lund University BMC C13
|
| City |
Lund |
| ZIP/Postal code |
SE-22184 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE31259 |
Gene Expression Profiling of Primary Male Breast Cancers Reveals Two Unique Subgroups and Identifies N-acetyltransferase-1 (NAT1) as a Novel Prognostic Biomarker |
|
