|
| Status |
Public on May 02, 2012 |
| Title |
30µM_amorfrutin1_1_expB |
| Sample type |
RNA |
| |
|
| Source name |
Amorfrutin1
|
| Organism |
Homo sapiens |
| Characteristics |
agent: Amorfrutin1
gender: female
developmental stage: differentiated
tissue: Subcutaneous adipose tissue
|
| Treatment protocol |
Compounds diluted in DMSO (0.1% final DMSO concentration), 24 hours at 37°C, 5% CO2
|
| Growth protocol |
According Zen-Bio's Human Subcutaneous Adipocyte/Preadipocyte Care Manual
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNeasy Mini Kit (QIAGEN) according to their manual. Genomic DNA was digested on column using the DNase-Set (QIAGEN). Quality control was performed with Agilent Bioanalyser
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina TotalPrep RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
replicate 1, experiment B
|
| Data processing |
The data were normalized using cubic spline normalization implemented in Illumina BeadStudio 3.3.
|
| |
|
| Submission date |
Apr 06, 2011 |
| Last update date |
May 02, 2012 |
| Contact name |
Christopher Weidner |
| E-mail(s) |
weidner@molgen.mpg.de
|
| Organization name |
Max Planck-Institute for Molecular Genetics
|
| Department |
Otto Warburg Laboratory
|
| Lab |
Sauer
|
| Street address |
Ihnestraße 63
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE28384 |
Amorfrutins are selective PPARγ agonists with potent antidiabetic properties |
|
