harvest date: 01-07-09 tissue: stem genotype: F7033 line, coming from "(LorenaXF252)XF252" genotype phenotype: yellow grain , Flint-Dent type
Treatment protocol
no treatment
Growth protocol
stem - Planting date: 21/04/2009 Pool de 5 plantes/ lignée/repetition conditions météo en Juillet pendant les prélèvements (station météo des Verrines) RG: Rayonnement global horaire (Joules/cm2) 202 T: Température instantanée horaraire (degrés Celsuis)20.27 U: humidité instantanée horaire (poucentage) 53
Extracted molecule
total RNA
Extraction protocol
ge2bl2:100ug.
Label
Biotin
Label protocol
labelling Biotin direct, amplification=yes, cRNA 20 ug.
Hybridization protocol
labelled extract quantity: 10ug
Scan protocol
GCOS, Biotin:pmt voltage 570nm,650V,laser power 1
Description
Maize, Zea mays, is one of the most widely grown crops in the world as it provides cereal grain for human purposes and feedstock for cattle. Maize is increasingly used as a biomass fuel, such as ethanol. Studies have shown that maize stem is made up of different lignified cell types: epidermis, sclerenchyma (a few layers under the epidermis and surrounding vascular bundles in the rind), parenchyma (mostly lignified near the epidermis). One of the aims of this work is to find out if it is the proportion of these different cell types or their number (especially for vascular bundles) which influences the degree of digestibility of maize lines. For that the objective of our work is to develop a genomic strategic approach allowing an improvement of traits involved in cell wall degradability. The work will include the mapping of genes considered as strategic in the cell wall biosynthesis, the search of new genes of interest through bio-analysis, the functional analysis of genes suspected to be of importance in cell wall pathway, the search of candidate underlying QTL, and transcriptomic approaches in order to find genes co-regulation, transcription.
Data processing
The data were normalized with the gcrma algorithm (Irizarry et al., 2003), available in the Bioconductor package (Gentleman and Carey, 2002).
