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Status |
Public on Sep 25, 2010 |
Title |
liver; Galactosamine; 25 mg/kg; 24 hours; 212519562_91705 |
Sample type |
RNA |
|
|
Source name |
Liver
|
Organism |
Rattus norvegicus |
Characteristics |
tissue: left lateral liver lobe strain: F344/N gender: male age: 10-12 weeks compound: Galactosamine hybdate: 7/27/2005 route: I.P. vehicle: PBS control: N dose: 25 mg/kg time of exposure: 24 hours necrosis score: 0 necrosis class: 0 necrosis level (linkon): 0 necrosis level (ekl): 0 institution: NIEHS/Cogenics
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Treatment protocol |
Rats were exposed for 6, 24 or 48 hours to a low, medium or high dose of one of eight hepatotoxicants. All animals were fasted for 12-18 hours prior to exposure.
|
Growth protocol |
F344/N male rats grow to 10-12 weeks old before exposed to one of eight hepatotoxicants
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from the flash frozen portion of the left lateral liver lobe using RNeasy Midi columns (Qiagen, Valencia, CA, USA) according to the manufacturer's protocol.
|
Label |
Biotin
|
Label protocol |
1 μg of total RNA from the left liver lobe of each individual rat was labeled using the GeneChip One-Cycle Target Labeling kit (Affymetrix, Inc., Santa Clara, CA, USA) following the manufacturer’s protocol.
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|
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Hybridization protocol |
The biotin-labeled cRNA was fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix, Inc.) for 17 hours, prior to washing and scanning.
|
Scan protocol |
Data were extracted from scanned images using the Affymetrix Scanner and GeneChip Operating Software (Affymetrix, Inc.).
|
Description |
Liver toxicants vs. non-toxicants based on overall necrosis score MAQC_Distribution_Status: MAQC_T -- Training
|
Data processing |
Affymetrix MAS5
|
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|
Submission date |
Sep 24, 2010 |
Last update date |
Sep 24, 2010 |
Contact name |
Leming Shi |
E-mail(s) |
lemingshi@fudan.edu.cn
|
Phone |
+86-18616827008
|
Organization name |
Fudan University
|
Department |
School of Life Sciences
|
Lab |
Center for Pharmacogenomics
|
Street address |
2005 Songhu Road
|
City |
Shanghai |
ZIP/Postal code |
200438 |
Country |
China |
|
|
Platform ID |
GPL1355 |
Series (2) |
GSE16716 |
MicroArray Quality Control Phase II (MAQC-II) Project |
GSE24363 |
MAQC-II Project: NIEHS data set |
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