|
| Status |
Public on Jan 01, 2011 |
| Title |
Peripheral blood leukocyte_Normal wieght mother_Sample 5 |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral blood leukocytes, Normal-weight mother in third trimester of pregnancy
|
| Organism |
Homo sapiens |
| Characteristics |
life stage: mother
tissue: peripheral blood
cell type: leukocytes
rna integrity score: 7.9
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from leukocyte samples using Ambion's Leukolocks kit. Quality control was performed using Agilent's Bioanalyzer.
|
| Label |
Cy3
|
| Label protocol |
cDNA and cRNA labeling and amplification were all performed using a single kit : Ambion's Illumina TotalPrep RNA Aamplification kit.
|
| |
|
| Hybridization protocol |
Illumina's BeadArray protocol
|
| Scan protocol |
Illumina's BeadArray protocol and BeadStudio (Gene expression Module)
|
| Description |
column in raw data file: NMM_5
pair with NMC_7
|
| Data processing |
Raw data was log2 transformed and median-centered using JMP Genomics 4.0 (SAS Institute, Cary NC).
|
| |
|
| Submission date |
Apr 14, 2010 |
| Last update date |
Jan 01, 2011 |
| Contact name |
Jinhee Kim |
| E-mail(s) |
jinhee.kim@northwestern.edu
|
| Organization name |
Northwestern University
|
| Department |
Infectious Disease
|
| Lab |
Martinelli
|
| Street address |
676 North St. Clair Street Arkes Suite 2330
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platform ID |
GPL6947 |
| Series (2) |
| GSE21342 |
Maternal influences on the transmission of leukocyte gene expression profiles in population samples (mother and child) |
| GSE21345 |
Maternal influences on the transmission of leukocyte gene expression profiles in population samples |
|
