|
| Status |
Public on Jun 29, 2016 |
| Title |
585A1-bFGF_20 |
| Sample type |
RNA |
| |
|
| Source name |
iPSCs, 20 ng/ml bFGF, 7 days
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: 585A1
cell type: induced pluripotent stem cells
treatment: 20 ng/ml basic FGF (bFGF) for 7 days
|
| Growth protocol |
The human induced pluripotent stem cells were cultured on SNL-feeder cells with Primate ESC medium (ReproCELL) supplemented with basic FGF and Activin A at 37 ºC in a humidified incubator with 5% CO2. In Samples 1-4, the cells were cultured with different concentrations of basic FGF (0, 4, 20, and 100 ng/ml) and without Activin A. In Samples 5-8, the cells were cultured with 4 ng/ml basic FGF and with different concentrations of Activin A (0, 4, 20, and 100 ng/ml).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the miRNeasy Purification kit (Qiagen) following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies).
|
| Label |
Cy3
|
| Label protocol |
Manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Manufacturer's protocol.
|
| Scan protocol |
Slides were scanned on the G2565CA Microarray Scanner System (Agilent).
|
| Description |
Sample 3
Human iPSC line (585A1) cultured with 20 ng/ml basic FGF (bFGF) for 7 days.
|
| Data processing |
GeneSpring GX12.1. Signals were normalized to the 75th percentile.
|
| |
|
| Submission date |
May 15, 2016 |
| Last update date |
Jun 29, 2016 |
| Contact name |
Yoshinori Yoshida |
| E-mail(s) |
yoshinor@cira.kyoto-u.ac.jp
|
| Organization name |
Kyoto University
|
| Street address |
53, Kawahara-cho, Shogoin, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
6068507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL14550 |
| Series (2) |
| GSE60924 |
Reprogramming-associated aberrant DNA methylation determines hematopoietic differentiation capacity of human induced pluripotent stem cells |
| GSE81453 |
Reprogramming-associated aberrant DNA methylation determines hematopoietic differentiation capacity of human induced pluripotent stem cells [iPSCs_expression_with_different_culture_conditions] |
|
