|
| Status |
Public on Aug 19, 2015 |
| Title |
Patient 11 - Non lesional skin-1 |
| Sample type |
RNA |
| |
|
| Source name |
Human skin
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: face biopsy
cell type: normal
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy (Qiagen) RNA extraction was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 300 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 µg of cRNA were hybridized for 16 hr at 45°C on U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using Affymetrix Gene Chip Scanner 3000.
|
| Data processing |
The data were analyzed with GEA model: The Global Error Assessment (GEA) model for the selection of differentially expressed genes in microarray data. Mansourian R1, Mutch DM, Antille N, Aubert J, Fogel P, Le Goff JM, Moulin J, Petrov A, Rytz A, Voegel JJ, Roberts MA.(2004) Bioinformatics Oxford Journals
|
| |
|
| Submission date |
Aug 18, 2015 |
| Last update date |
Aug 19, 2015 |
| Contact name |
CORINNE MENIGOT |
| E-mail(s) |
corinne.menigot@galderma.com
|
| Organization name |
GALDERMA R & D
|
| Street address |
2400 route des colles
|
| City |
Sophia Antipolis |
| ZIP/Postal code |
06902 |
| Country |
France |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE72140 |
Transcriptional profiling shows altered expression of wnt pathway- and lipid metabolism-related genes as well as melanogenesis-related genes in melasma. |
|
