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Series GSE72140 Query DataSets for GSE72140
Status Public on Aug 19, 2015
Title Transcriptional profiling shows altered expression of wnt pathway- and lipid metabolism-related genes as well as melanogenesis-related genes in melasma.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Melasma is a commonly acquired hyperpigmentary disorder of the face, but its pathogenesis is poorly understood and its treatment remains challenging. We conducted a comparative histological study on lesional and perilesional normal skin to clarify the histological nature of melasma. Significantly, higher amounts of melanin and of melanogenesis-associated proteins were observed in the epidermis of lesional skin, and the mRNA level of tyrosinase-related protein 1 was higher in lesional skin, indicating regulation at the mRNA level. However, melanocyte numbers were comparable between lesional and perilesional skin. A transcriptomic study was undertaken to identify genes involved in the pathology of melasma. A total of 279 genes were found to be differentially expressed in lesional and perilesional skin. As was expected, the mRNA levels of a number of known melanogenesis-associated genes, such as tyrosinase, were found to be elevated in lesional skin. Bioinformatics analysis revealed that the most lipid metabolism-associated genes were downregulated in lesional skin, and this finding was supported by an impaired barrier function in melasma. Interestingly, a subset of Wnt signaling modulators, including Wnt inhibitory factor 1, secreted frizzled-related protein 2, and Wnt5a, were also found to be upregulated in lesional skin. Immunohistochemistry confirmed the higher expression of these factors in melasma lesions.
 
Overall design Total of 48 chips. 12 patients : 2 biospies per patient: 1 lesional and 1 non lesional: 1 technical replicate per chip
 
Contributor(s) Voegel JJ
Citation(s) 21562572
Submission date Aug 18, 2015
Last update date Mar 25, 2019
Contact name CORINNE MENIGOT
E-mail(s) corinne.menigot@galderma.com
Organization name GALDERMA R & D
Street address 2400 route des colles
City Sophia Antipolis
ZIP/Postal code 06902
Country France
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (48)
GSM1855916 Patient 1 - Non lesional skin-1
GSM1855917 Patient 1 - Non lesional skin-2
GSM1855918 Patient 1 - Lesional skin-1
Relations
BioProject PRJNA293168

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE72140_RAW.tar 218.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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