|
| Status |
Public on Nov 20, 2012 |
| Title |
FL2 CNTRL siRNA untreated repeat 1 |
| Sample type |
RNA |
| |
|
| Source name |
FL2 CNTRL siRNA untreated repeat 1
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: normal diploid human fetal lung fibroblast
transfection: FL2 CNTRL siRNA
|
| Treatment protocol |
Exponentially growing cells treated with 200ng/mL doxorubicin or sham for 26 hours.
|
| Growth protocol |
Cells were propogated according to standard cell culture conditions for indicated cell lines.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated by Trizol extraction and Qiagen RNAeasy spin column. DNA contamination eliminated by DNAse treatment. RNA quality was evaluated by Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
biological replicate 1
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Nov 19, 2012 |
| Last update date |
Sep 13, 2016 |
| Contact name |
Adam Schmitt |
| E-mail(s) |
amschmit@stanford.edu
|
| Phone |
650-736-0305
|
| Fax |
650-723-8762
|
| Organization name |
Stanford University
|
| Department |
Department of Dermatology
|
| Lab |
Howard Y. Chang
|
| Street address |
269 Campus Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5168 |
| Country |
USA |
| |
|
| Platform ID |
GPL6947 |
| Series (2) |
| GSE42368 |
p53-dependent regulation of gene expression following DNA damage |
| GSE76420 |
LncRNA DINO guides DNA damage signaling |
|
