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Series GSE39879 Query DataSets for GSE39879
Status Public on Feb 05, 2013
Title FoxA1 specifies unique androgen and glucocorticoid receptor binding events in prostate cancer cells (HTS)
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We report the androgen receptor recruitment to the chromatin of androgen responsive prostate cancer cell lines, LNCaP-1F5 and VCaP in response to physiological androgen 5a-dihydrotestosterone (DHT) using ChIP-sequencing. We compare the AR recruitment by DHT to that by partial agonist/antagonist cyproterone acetate (CPA), mifepristone (RU486) and bicalutamide (Bica) in LNCaP-1F5 cells. We also report the role of glucocorticoid receptor recruitment in presence of dexamethasone (Dex) in androgen responsive prostate cancer cells. The AR and GR cistrome analysis is subsequently compared with gene expression data and RNA Pol II analysis. The ChIP-seq has been performed using AR, GR, RNA Pol II antibodies.
Overall design Examination of AR and GR binding sites in LNCaP-1F5 and VCaP cells in presence of DHT and Dex respectively. Further analysis of AR binding sites in LNCaP-1F5 cells treated with partial agonist/antagonists, CPA, RU486 and Bica. Additionally RNA Pol II mapping is performed in cells treated with DHT and Dex.
Contributor(s) Biswajyoti S, Marko L, Paivi P, Kristian O, Ievgenii S, Sampsa H, Olli JA
Citation(s) 23269278
Submission date Aug 03, 2012
Last update date May 15, 2019
Contact name Olli A. Jänne
Phone +358919125040
Organization name University of Helsinki
Department Inst. of Biomedicine/Physiology
Lab Androgen Receptor Laboratory
Street address Haartmaninkatu-8
City Helsinki
ZIP/Postal code FI-00014
Country Finland
Platforms (2)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
Samples (22)
GSM759657 AR_ChIP-seq_rep1
GSM759658 AR_ChIP-seq_rep2
GSM759671 rIgG ChIP-seq
This SubSeries is part of SuperSeries:
GSE39880 FoxA1 specifies unique androgen and glucocorticoid receptor binding events in prostate cancer cells
BioProject PRJNA171947
SRA SRP014685

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE39879_RAW.tar 2.0 Gb (http)(custom) TAR (of BED, SAM, TXT, WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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