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Series GSE27219 Query DataSets for GSE27219
Status Public on Mar 07, 2012
Title The condition-dependent transcriptome of Bacillus subtilis 168
Organism Bacillus subtilis subsp. subtilis str. 168
Experiment type Expression profiling by genome tiling array
Summary Recent studies revealed the unsuspected complexity of the bacterial transcriptome but its systematic analysis across many diverse conditions remains a challenge. Here we report the condition-dependent transcriptome of the prototype strain B. subtilis 168 across 104 conditions reflecting the bacterium's life-styles. This data set composed of 269 tiling array hybridizations allowed to observe ~85% of the annotated CDSs expressed in the higher 30% in at least one hybridization and thus provide an excellent coverage of the transcriptome of this bacterium. In addition to the Genbank annotation 1583 new segments of the chromosome were identified as transcribed and have transcription levels reported in the data matrix.
Overall design RNA samples prepared from B. subtilis 168 grown under 104 experimental conditions were reverse transcribed, labeled and hybridized to tiling arrays. Most hybridizations were performed in duplicate or triplicate using RNA isolated from independent cultures. All experiments were performed with strain BSB1 which is a tryptophan-prototrophic (trp+) derivative of the 168 trpC2 strain.
Contributor(s) Mäder U, Dervyn E, Nicolas P, Botella E, Härtig E, Le Chat L, Matthieu J, Pohl S, Rochat T, Sappa PK, Schaffer M
Citation(s) 22383849
Submission date Feb 10, 2011
Last update date Apr 07, 2014
Contact name Ulrike Mäder
Organization name University Medicine Greifswald
Department Functional Genomics
Street address F.-L.-Jahn-Str. 15A
City Greifswald
ZIP/Postal code D-17489
Country Germany
Platforms (1)
GPL13149 BaSysBio Bacillus subtilis T1 385K array [070910_BaSysBio_expr]
Samples (269)
GSM672549 LBexp_1
GSM672550 LBexp_2
GSM672551 LBexp_3
BioProject PRJNA137513

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE27219_RAW.tar 2.1 Gb (http)(custom) TAR (of PAIR)
Processed data included within Sample table

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