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| Status |
Public on Mar 07, 2012 |
| Title |
The condition-dependent transcriptome of Bacillus subtilis 168 |
| Organism |
Bacillus subtilis subsp. subtilis str. 168 |
| Experiment type |
Expression profiling by genome tiling array
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| Summary |
Recent studies revealed the unsuspected complexity of the bacterial transcriptome but its systematic analysis across many diverse conditions remains a challenge. Here we report the condition-dependent transcriptome of the prototype strain B. subtilis 168 across 104 conditions reflecting the bacterium's life-styles. This data set composed of 269 tiling array hybridizations allowed to observe ~85% of the annotated CDSs expressed in the higher 30% in at least one hybridization and thus provide an excellent coverage of the transcriptome of this bacterium. In addition to the Genbank annotation 1583 new segments of the chromosome were identified as transcribed and have transcription levels reported in the data matrix.
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| Overall design |
RNA samples prepared from B. subtilis 168 grown under 104 experimental conditions were reverse transcribed, labeled and hybridized to tiling arrays. Most hybridizations were performed in duplicate or triplicate using RNA isolated from independent cultures. All experiments were performed with strain BSB1 which is a tryptophan-prototrophic (trp+) derivative of the 168 trpC2 strain.
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| Contributor(s) |
Mäder U, Dervyn E, Nicolas P, Botella E, Härtig E, Le Chat L, Matthieu J, Pohl S, Rochat T, Sappa PK, Schaffer M |
| Citation(s) |
22383849 |
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| Submission date |
Feb 10, 2011 |
| Last update date |
Apr 07, 2014 |
| Contact name |
Ulrike Mäder |
| E-mail(s) |
ulrike.maeder@uni-greifswald.de
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| Organization name |
University Medicine Greifswald
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| Department |
Functional Genomics
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| Street address |
F.-L.-Jahn-Str. 15A
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| City |
Greifswald |
| ZIP/Postal code |
D-17489 |
| Country |
Germany |
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| Platforms (1) |
| GPL13149 |
BaSysBio Bacillus subtilis T1 385K array [070910_BaSysBio_expr] |
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| Samples (269)
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| Relations |
| BioProject |
PRJNA137513 |
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